Continuous free flow electrophoresis device composed of a plurality of specific sub-chambers separated by films

A specific and electrophoretic technology, applied in the field of continuous free-flow electrophoresis equipment, which can solve the problems of imperfection, inability to precisely control liquid flow, and unstable effect.

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A specific and electrophoretic technology, applied in the field of continuous free-flow electrophoresis equipment, which can solve the problems of imperfection, inability to precisely control liquid flow, and unstable effect.

CN101011643AInactive Publication Date: 2007-08-08FUJIAN MEDICAL UNIV

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[0019] The electrophoresis device of the present invention includes some independent components containing corresponding electrophoresis chambers, and the components belonging to each electrophoresis chamber can be combined and used in different ways according to the needs of separating different proteins.

[0020] The present invention may include two-stage electrophoresis equipment, the first stage is for isoelectric point electrophoresis, and the second stage is for synthesis of isoelectric point electrophoresis and electroultrafiltration, and the two-stage electrophoresis can be used sequentially or separately.

[0021] The negative and positive electrode chambers of two-stage electrophoresis and the membranes between other chambers are all dialysis membranes with a molecular weight cut-off of 500-1000. The pore radius of this type of membrane is less than 1nm, which not only has a large liquid flow resistance, but also can produce an electroosmotic diffusion layer. Almost ...

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Abstract

The invention discloses continuous free-flow electrophoretic equipment separated by film, which comprises the following parts: main electrophoretic separating chamber, positive and negative electrode chambers and complex protein chamber, wherein the complex protein chamber interconnects inlet and outlet pipes of closed buffer circulating pipe to control transmembrane flow; the buffer updating mechanism contains double-dynamic pump, liquid-container and fresh buffer container with one end extending into inlet and outlet pipes to supplement and drain liquid; the other end of inlet and outlet pipes interconnect liquid feed valve of liquid-supplementing chamber of double-dynamic pump and draining valve of liquid draining chamber; the fresh buffer container and liquid-container interconnect liquid feed valve and liquid exhaust chamber of double-dynamic pump liquid-supplementing chamber; the device can separate and purify protein according to isoelectric point and molecular weight.

Description

Technical field: [0001] The invention relates to a continuous free-flow electrophoresis device for separating protein bioactive substances. Background technique: [0002] Continuous free-flow electrophoresis is a preparative-scale electrophoresis method that can continuously inject samples and continuously collect products. Currently, continuous free-flow electrophoresis for separating proteins mainly includes free-flow zone electrophoresis and free-flow isoelectric focusing. [0003] In free-flow zone electrophoresis, the protein-containing liquid is continuously added to one end of the separation chamber from the direction perpendicular to the electric field, and the proteins with different mobility in the sample obtain different migration speeds parallel to the direction of the electric field and The same moving speed perpendicular to the direction of the electric field is obtained under the continuous input of the liquid flow, so the proteins with different mobility will...

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Application Information

Patent Timeline

08 Aug 2007

Publication

CN101011643A

IPC: B01D57/02; G01N27/447; C07K1/26

Inventors: 陈炜; 倪秀雄