The invention discloses a preparation method of a natural chicken essence seasoning. The method comprises the following steps: preparing raw materials: firstly, selecting a raw material chicken, slaughtering the raw material chicken, then performing soaking in hot water for a period of time, then performing cleaning and feather picking, cutting the raw material chicken into blocks after cleaning is completed to obtain raw material chicken blocks to be treated, putting the raw material chicken blocks to be treated into a high-pressure cooking pot, and carrying out sealed cooking. Through the preparation of the chicken essence seasoning, leftovers in broiler chicken processing can be fully utilized, economic benefits of enterprises are improved, an advanced process can be applied to the actual production of broiler chicken processing, a development direction is provided for the further development of meat deep processing, and problems that in the prior art, the main raw material for producing the chicken essence seasoning is chicken, and leftovers such as chicken bones and meat in broiler chicken processing are discarded, so that the fragrance, taste and nutritional ingredients of natural chicken are lost, and therefore the production cost of enterprises is increased are solved.