Use of serratia marcesens bacterial vaccine for preparing drug for treating lung fibrosis

Abstract

The present invention discloses an application of serratia marcescens vaccine in preparation of medicine for curing pulmonary fibrosis. The animal tests show that said serratia marcescens vaccine has obvious action for curing pulmonary fibrosis. As compared with hormone said serratia marcescens has incomparable safety.

Description

technical field

[0001] The invention relates to the use of the Serratia marcescens bacterin, in particular to the use of the Serratia marcescens bacterin in the pharmaceutical field. Background technique

[0002] The bacteriological characteristics of the Serratia marcescens bacterin and relevant information have been described in detail in the document disclosed in the CN02111996.1 patent.

[0003] The bacterin is Gram-negative bacilli with flagella all over the body, motility, no encapsulation and spores. The bacterium grows well on ordinary medium at 37°C under general aerobic conditions. On ordinary agar plates, the colonies are smooth and moist. Raised, with neat edges and round shape, biochemical reaction: it can ferment glucose, sucrose, mannitol, amygdalin, produce acid but not gas. Non-fermentable rhamnose, arabinose, and closebiose. Can produce extracellular deoxyribonuclease. Species number: CGMCCNo.0744, classification name: Serratia marcescens.

[0004] CN02...

Examples

Embodiment 1

[0019] Cultivation and collection of Serratia marcescens vaccine:

[0020] The working seeds for production are grown in solid state. After inoculating the working seeds for production in the nutrient agar medium, inoculate for 2 consecutive subcultures, and finally inoculate the slant of the nutrient agar medium in the Koch flask, and culture aerobically at 37°C for 19 hours, no more than 4 generations. During the cultivation, there is no special requirement on the cultivation conditions, whichever is suitable for the growth of the Serratia marcescens vaccine, the pH range is 2-9, and the temperature is 4-40°C. The pH value of this experiment is 6, and the temperature is 20°C. Microscopically inspect each bottle before collection, and discard those contaminated with bacteria. The collected bacterial lawn was placed in sterilized saline for injection, mixed thoroughly, and filtered with four layers of silk cloth. Measured according to the Chinese bacterial turbidity standar...

Embodiment 2

[0023] Get 1000ml of pyrogen-free deionized pure distilled water to dissolve 640mg of Serratia marcescens bacterin, mix evenly, sterilize and configure into 0.64mg / ml injection, bottle to make finished product.

Embodiment 3

[0025] The specific experiments and results are described as follows:

[0026] 1 Materials and methods

[0027] 1.1 Reagents and instruments Bleomycin (Pingyangmycin hydrochloride produced by Tianjin Hebei Pharmaceutical Factory, hereinafter referred to as BLM) was dissolved in normal saline to prepare 5 mg / ml.

[0028] 1.2 Animal grouping and treatment methods 80 healthy SD rats (provided by the Department of Experimental Zoology, Second Military Medical University), male or female, weighing (220±28) g. Among them, 48 rats were randomly divided into groups after intratracheal injection of BLMA55mg / kg at one time. ① Model group (24 rats): 0.3ml of normal saline was instilled into the nasal cavity of each rat from the next day after model preparation; ② Serratia marcescens vaccine group (24 rats): From the next day after model preparation, 0.3ml (0.01mg / kg) of Serratia marcescens vaccine was instilled into the nasal cavity of each rat every day for 28 days; ③Normal control gro...

technical field

[0001] The invention relates to the use of the Serratia marcescens bacterin, in particular to the use of the Serratia marcescens bacterin in the pharmaceutical field. Background technique

[0002] The bacteriological characteristics of the Serratia marcescens bacterin and relevant information have been described in detail in the document disclosed in the CN02111996.1 patent.

[0003] The bacterin is Gram-negative bacilli with flagella all over the body, motility, no encapsulation and spores. The bacterium grows well on ordinary medium at 37°C under general aerobic conditions. On ordinary agar plates, the colonies are smooth and moist. Raised, with neat edges and round shape, biochemical reaction: it can ferment glucose, sucrose, mannitol, amygdalin, produce acid but not gas. Non-fermentable rhamnose, arabinose, and closebiose. Can produce extracellular deoxyribonuclease. Species number: CGMCCNo.0744, classification name: Serratia marcescens.

[0004] CN02...

Examples

Embodiment 1

[0019] Cultivation and collection of Serratia marcescens vaccine:

[0020] The working seeds for production are grown in solid state. After inoculating the working seeds for production in the nutrient agar medium, inoculate for 2 consecutive subcultures, and finally inoculate the slant of the nutrient agar medium in the Koch flask, and culture aerobically at 37°C for 19 hours, no more than 4 generations. During the cultivation, there is no special requirement on the cultivation conditions, whichever is suitable for the growth of the Serratia marcescens vaccine, the pH range is 2-9, and the temperature is 4-40°C. The pH value of this experiment is 6, and the temperature is 20°C. Microscopically inspect each bottle before collection, and discard those contaminated with bacteria. The collected bacterial lawn was placed in sterilized saline for injection, mixed thoroughly, and filtered with four layers of silk cloth. Measured according to the Chinese bacterial turbidity standar...

Embodiment 2

[0023] Get 1000ml of pyrogen-free deionized pure distilled water to dissolve 640mg of Serratia marcescens bacterin, mix evenly, sterilize and configure into 0.64mg / ml injection, bottle to make finished product.

Embodiment 3

[0025] The specific experiments and results are described as follows:

[0026] 1 Materials and methods

[0027] 1.1 Reagents and instruments Bleomycin (Pingyangmycin hydrochloride produced by Tianjin Hebei Pharmaceutical Factory, hereinafter referred to as BLM) was dissolved in normal saline to prepare 5 mg / ml.

[0028] 1.2 Animal grouping and treatment methods 80 healthy SD rats (provided by the Department of Experimental Zoology, Second Military Medical University), male or female, weighing (220±28) g. Among them, 48 rats were randomly divided into groups after intratracheal injection of BLMA55mg / kg at one time. ① Model group (24 rats): 0.3ml of normal saline was instilled into the nasal cavity of each rat from the next day after model preparation; ② Serratia marcescens vaccine group (24 rats): From the next day after model preparation, 0.3ml (0.01mg / kg) of Serratia marcescens vaccine was instilled into the nasal cavity of each rat every day for 28 days; ③Normal control gro...

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