Analysis method for detecting activity of nitrous acid reductase in soil
An analysis method, the technology of nitrous acid, is applied in the direction of material analysis by observing the influence on chemical indicators, biochemical equipment and methods, and analysis by making materials undergo chemical reactions, etc., which can solve the cumbersome operation steps and difficulty in adapting to the soil Qualitative comparison and quantitative research of nitrite reductase activity, uncertainty of anaerobic culture degree, etc., to achieve the effect of simplified operation steps, low cost and easy operation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0033] 7. Preparation of standard curve: draw 1ml of the above-mentioned standard stock solution, and dilute to 100ml, which is 2.5mgNO 2 - -N / L solution. Then draw 0, 1, 2, 3, 4, and 5ml of the solution into a 50ml volumetric flask, add a small amount of distilled water and 4ml of developer, and distill to volume. The amount of nitrite nitrogen contained in this standard series is 0, 0.05, 0.1, 0.15, 0.2, 0.25mgNO 2 - -N / L. Colorimetric determination of a range of known concentrations of NaNO at 520nm 2 The absorbance value A1 of the solution, expressed as NaNO 2 The concentration of the solution and the measured absorbance value A' prepare a standard curve to obtain the slope b;
[0034] Steps:
[0035] 1. Weigh 3 parts of 1g soil air-dried sample passed through a 1mm sieve into 3 test tubes, add 20mg CaCO respectively 3 (For calcareous soils, CaCO 3 No need to add, because the CaCO in the soil itself 3The amount can meet the optimum pH of microorganisms), after ca...
Embodiment 1
[0043] Select four soil samples that have been treated differently, of which 1 is the control, and the amount of a certain compound added to samples 2, 3, and 4 is sequentially increased. At a temperature of 25°C, the soil moisture content remains at 20% (calculated on an oven-dried basis) After culturing for 1 week under certain conditions, the effect of the added amount of the compound on the activity of soil nitrite reductase was detected. The specific analysis steps are as follows:
[0044] 1. Weigh 3 parts of 1g soil air-dried sample passed through a 1mm sieve into 3 test tubes, add 20mg CaCO respectively 3 , After mixing carefully, add 2ml of 0.25% sodium nitrite solution to two of the test tubes, and add the same amount of distilled water to the other test tube as a sample control. Then 2ml of 0.5% glucose solution was added to the 3 test tubes as a hydrogen donor, and then 2ml of distilled water was added, plugged with an airtight rubber stopper, shaken well, and incu...
Embodiment 2
[0053] The determination of nitrite reductase activity was carried out on four differently treated soil samples, and the specific analysis steps were as follows: the substrate NaNO 2 The concentration of glucose is 0.20%, add 2ml; The concentration of glucose is 1%, add 1ml, then add 5ml distilled water, add color developer 2 when developing color, all the other operation steps are the same as embodiment 1. The test results are as follows:
[0054]
[0055] The data in the table also showed the stability of the analysis results, and the differences among the treatments reached extremely significant level.
PUM
| Property | Measurement | Unit |
|---|---|---|
| diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More