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Application of honokiol in preparing medicine for treating acute leukemia

A technology of honokiol and leukemia, which is applied in the field of natural compounds, can solve the problems of being unable to inhibit, and achieve the effect of low toxicity

Inactive Publication Date: 2009-07-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Another example is Mitotic catastrophe, this death is caused by the failure of mitosis, accompanied by the permeabilization of mitochondrial membrane and the activation of caspases, but it is different from apoptosis, because neither the inhibition of caspases nor the overexpression of Bcl-2 can Inhibit the progress of this mode of death

Method used

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  • Application of honokiol in preparing medicine for treating acute leukemia
  • Application of honokiol in preparing medicine for treating acute leukemia
  • Application of honokiol in preparing medicine for treating acute leukemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Honokiol can kill leukemia cells, and this effect can be inhibited by CsA

[0022] Experimental materials: primary AML and ALL (acute lymphoid leukemia) mononuclear cells are derived from the bone marrow blood of acute phase AML and ALL patients, and the proportion of immature cells is greater than 85% by Giemsa staining of marrow slices. Honokiol was purchased from China National Institute of Pharmaceuticals and Biological Products. Propidium iodide (PI), total caspase inhibitor (z-VAD-fmk), cyclosporin A (CsA), and Ficoll lymphocyte separation medium were purchased from Sigma.

[0023] experimental method:

[0024] 1. (1) Use Ficoll separation medium to separate mononuclear cells from the patient's bone marrow blood, and check the viability with trypan blue. (2) Recovery of mononuclear cells with 1640 medium containing 10% calf serum at 37°C for one hour. (3) 1×10 6 / ml cell plate, all the cells were divided into two groups, one group was pre-added 5μM ...

Embodiment 2

[0030] Example 2: Toxic effect of honokiol on peripheral blood mononuclear cells of healthy people

[0031] Experimental Materials:

[0032] The mononuclear cells of healthy people were all derived from the peripheral blood of healthy volunteers, and the proportion of immature cells was greater than 85% by Giemsa staining of marrow slices. Honokiol was purchased from China National Institute of Pharmaceuticals and Biological Products. Propidium iodide (PI), cyclosporine A (CsA), and Ficoll lymphocyte separation medium were purchased from Sigma.

[0033] experimental method:

[0034] (1) Separate mononuclear cells from the patient's bone marrow blood with Ficoll separation medium, and check the viability with trypan blue. (2) Recovery of mononuclear cells with 1640 medium containing 10% calf serum at 37°C for one hour. (3) 1×10 6 / ml cell plate, treated mononuclear cells with 10, 20, 30, 40, 50, 60, 80 μg / ml honokiol for 48 hours. (4) After the treatment, the cells were col...

Embodiment 3

[0037] Example 3: Honokiol kills acute leukemia cells through non-classic apoptosis

[0038] Experimental Materials:

[0039] The mononuclear cells of healthy people were all derived from the peripheral blood of healthy volunteers, and the proportion of immature cells was greater than 85% by Giemsa staining of marrow slices. Honokiol was purchased from China National Institute of Pharmaceuticals and Biological Products. Propidium iodide (PI), total caspase inhibitor (z-VAD-FMK), cyclosporin A (CsA), and Ficoll lymphocyte separation medium were purchased from Sigma.

[0040] experimental method:

[0041] (1) Separate mononuclear cells from the patient's bone marrow blood with Ficoll separation medium, and check the viability with trypan blue. (2) Recovery of mononuclear cells with 1640 medium containing 10% calf serum at 37°C for one hour. (3) 1×10 6 / ml cell seed plate, treated mononuclear cells with 30, 40, 50 μg / ml honokiol for 24 hours, wherein the VP-16 100 μg / ml grou...

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PUM

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Abstract

The invention provides the use of honokiol in the preparation of medicines for treating leukemia. The molecular weight of the honokiol is 266.33, its chemical name is 3,5'-diallyl-4,2'-dihydroxybiphenyl, and its molecular formula is C18H18O2. The honokiol medicine prepared by the present invention includes pharmaceutical excipients or carriers allowed by the preparation, and is made into a dosage form of enteral or parenteral combination medicine according to known methods in the preparation field, and the preparation forms mainly include liquid preparations, granules, tablets formulations, sustained-release preparations, granules, gelatin pills, capsules, drop pills or injections. The invention can effectively treat this type of leukemia through the non-classical apoptosis pathway. Honokiol has low toxicity, but currently used clinical antineoplastic drugs are highly toxic to the human body. Therefore, honokiol has the application prospect of preparing clinical anti-leukemia drugs.

Description

technical field [0001] The invention belongs to the use of natural compounds, and relates to the new use of honokiol extracted from plants, especially the use in the preparation of medicines for treating acute leukemia. Background technique [0002] Honokiol is a natural compound extracted from the plant Magnolia officinalis. Its chemical structure is as follows: [0003] [0004] Honokiol has been proved to be absorbed by the gastrointestinal tract during in vivo experiments such as its antithrombotic and anti-anxiety, and has characteristics such as long retention time in the body and no obvious side effects (Teng CM, Chen CC, Ko FN, et al. al. Thromb Res. 1988; 50: 757-765, Watanabe K, Watanabe H, Goto Y, et al. Planta Med. 1983; 49: 103-108) [0005] In June 1946, the first foreign case of leukemia treated with chemotherapy drugs achieved remission, opening up a new era of leukemia treatment. After the 1970s, strategies such as combined chemotherapy, maintenance, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/05A61K45/06A61K9/08A61K9/10A61K9/14A61K9/16A61K9/20A61P35/02
Inventor 胡汛李凌
Owner ZHEJIANG UNIV
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