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Shiraia strain for perylene producing quinone compound

A compound, the technology of bamboo yellow fungus, which is applied in the field of bioengineering, can solve the problems of instability, cannot be applied to industrialized production, and low yield of rhodopsin, and achieves the effect of improving the ability of perylene quinone compounds

Inactive Publication Date: 2009-08-12
江苏竹红生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] However, the reported hypocretin yield is low and unstable. These reports have not carried out follow-up further research and cannot be applied to industrial production.

Method used

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  • Shiraia strain for perylene producing quinone compound
  • Shiraia strain for perylene producing quinone compound
  • Shiraia strain for perylene producing quinone compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The fermentation strain is Shiraia sp. SUPER-H168, which adopts solid-state fermentation, and the medium composition (weight%): 60 mesh corn flour 68.7, bran 10, rice bran 20, glucose 1, KH 2 PO 4 0.25, MgSO 4 7H 2 O0.05; add water and mix to moisten, put into a jar, sterilize at 0.1MPa for 45-60min, add liquid seeds after cooling and mix well. The fermentation temperature is 30° C., the pH is 6, and after 10 days of fermentation, the yield of perylenequinone compounds reaches about 0.8%, of which 40% of hypocrellin A and 55% of hypocrellin B are produced.

Embodiment 2

[0039] The fermentation strain is Shiraia sp. SUPER-H168, which is fermented in a liquid state, using a standard Chapei culture medium, with a fermentation temperature of 28° C. and a dissolved oxygen content of 25% during the fermentation process. After 3 days of fermentation, the yield of perylenequinone compounds reached about 0.07%, of which 48% for hypocrellin A and 43% for hypocrellin B.

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Abstract

A bamboo yellow strain producing perylene quinone compounds belongs to the technical field of bioengineering. The bacterial strain of the present invention is classified as Shiraia sp. SUPER-H168, and has been preserved in the China Center for Type Culture Collection with the preservation number CCTCC M 207104. The strain was isolated and screened from the fruiting body of Bamboo quinquefolium growing on Mashan danhui bamboo in Wuxi, Jiangsu Province, and obtained a strain of Bamboo quinone with stable properties and producing perylene quinone compounds, which can produce perylene quinone compounds through solid-state or liquid-state fermentation .

Description

technical field [0001] A bamboo yellow strain producing perylene quinone compounds belongs to the technical field of bioengineering. The invention relates to a bamboo yellow bacterial strain with high yield of perylene quinone compounds, which is of great significance to the industrial production of perylene quinone compounds. Background technique [0002] In nature, many fungi of different species can produce metabolites with a perylenequinone skeleton (Li Cong, Xie Jinlun. Analysis and comparison of chemical components of three medicinal fungi of the family Hypocreataceae. Chinese herbal medicine. 2000, 31 (4): 250 .), and in recent years, most of the research work has focused on the photodynamics and stereochemistry of hypocretin, cercosporine and hypericin. Among them, hypocrellin has a series of characteristics such as good stability, no obvious side effects when taken orally, fast metabolism, soluble in many organic solvents and insoluble in water. As an important na...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N15/31C12P7/26
Inventor 蔡宇杰廖祥儒梁晓辉金大勇王蕾丁彦蕊孟强张大兵
Owner 江苏竹红生物科技有限公司
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