Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for extracting melanin from black-bone chicken

A technology for silky chicken and melanin, which is applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problems of long time for extracting melanin, complex enzymatic hydrolysis conditions, complicated steps, etc. Simple, cost-effective effect

Inactive Publication Date: 2009-08-19
CHINA AGRI UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Disadvantages are high cost, complex enzymatic hydrolysis conditions, cumbersome steps, and long time to extract melanin

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting melanin from black-bone chicken
  • Method for extracting melanin from black-bone chicken
  • Method for extracting melanin from black-bone chicken

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The method for extracting melanin from silky chickens comprises chopping the silky chickens, washing them with 6M hydrochloric acid solution for 10 minutes, and immersing them in liquid nitrogen for 1 minute. Crush the black-bone chicken immersed in liquid nitrogen into 80-mesh powder, transfer it to a Soxhlet extractor, and reflux with 6M hydrochloric acid solution for 2 hours to stop. The amount of hydrochloric acid solution used meets the reflux conditions, and the extract is cooled to Precipitate was precipitated at 4°C, and the precipitate was first degreased and washed 4 times with acetone equal to the volume of the extract, then washed 4 times with distilled water equal to the volume of the extract, and dried.

Embodiment 2

[0020] The method for extracting melanin from black-bone chicken comprises chopping the black-bone chicken, washing with 1M hydrochloric acid solution for 30 minutes, and immersing in liquid nitrogen for 5 minutes. Crush the black-bone chicken immersed in liquid nitrogen into 100-mesh powder, transfer it to a Soxhlet extractor, and then stop it by refluxing with 1M hydrochloric acid solution for 3 hours. The amount of hydrochloric acid solution used meets the reflux conditions, and the extract is cooled to Precipitate was precipitated at 5°C, and the precipitate was degreased and washed 3 times with acetone equal to the volume of the extract, then washed 3 times with distilled water equal to the volume of the extract, and dried.

Embodiment 3

[0022] The method for extracting melanin from silky chickens comprises chopping the silky chickens, washing them with 10M hydrochloric acid solution for 5 minutes, and immersing them in liquid nitrogen for 0.5 minutes. Crush the black-bone chicken immersed in liquid nitrogen into 60-mesh powder, transfer it to a Soxhlet extractor, and then stop it by refluxing with 10M hydrochloric acid solution for 1.5 hours. The amount of hydrochloric acid solution used meets the reflux conditions, and the extract is cooled to Precipitate is precipitated at 0°C, and the precipitate is degreased and washed 3 times with acetone equal to the volume of the extract, then washed 3 times with distilled water equal to the volume of the extract, and dried.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to a method for extracting melanin from black-bone chickens, which comprises the following steps: after the black-bone chickens are chopped, they are washed with 1-10M hydrochloric acid solution for 5-30 minutes, immersed in liquid nitrogen for 0.5-5 minutes, and soaked in liquid nitrogen The final black-bone chicken was crushed into 60-100 mesh powder, transferred to a Soxhlet extractor, refluxed with 1-10M hydrochloric acid solution for 1.5-3 hours, and the extract was cooled to 0-5°C to precipitate a precipitate, and then added with The extract is washed with acetone distilled water of equal volume for 3 to 4 times, and dried. The method of the present invention can fully and effectively destroy the structure of melanocytes in the tissue by freezing and pulverizing the melanin without changing the physical and chemical properties of the melanin, which is conducive to the rapid release of the melanin and shortens the time for extracting the melanin. The time used keeps the nearly complete morphological structure of the black-bone chicken melanin.

Description

technical field [0001] The invention relates to a method for extracting melanin, in particular to a method for extracting melanin from silky chicken. Background technique [0002] Silkie is a unique rare bird in my country, which has special nourishing and medicinal value. In "Compendium of Materia Medica", Li Shizhen said, "Silky chickens include white-haired black-boned chickens, black-haired black-boned chickens, spotted-haired black-boned chickens, black-boned chickens and white meat and black-boned chickens, but the tongue is black. Those who have it will have black bones and flesh, and it will be better when used as medicine." It can be seen that the amount of melanin in the meat and bones is an important criterion for ancient medicine to distinguish the medicinal effect of silky chicken. Modern medicine also believes that the medicinal value of silky chicken lies in the melanin substance in its body. Melanin is a complex and irregular polymer widely present in many...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C09B61/00
Inventor 杨宁陈思睿蒋斌郑江霞
Owner CHINA AGRI UNIV