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Anti AIDS 1 type virus compound preparation method

A typical, culture-based technology, applied in the direction of drug combination, allergic diseases, bacteria, etc., to achieve the effect of simple operation method

Inactive Publication Date: 2010-02-24
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The biological resources in the deep sea have not yet been developed, and it is more likely to find new medicinally active compounds from the new strains. Therefore, the present invention provides a new deep-sea bacterium and a compound with anti-HIV type I preparation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Prepare 101X strain fermentation broth. Use MM medium for the deposited strain 101X: (NH4)2SO4 0.1%, K2HPO4 0.7%, KH2PO4 0.3%, sodium citrate 0.05%, MgSO4 0.01%, glucose 0.5%, agar 2.0%, distilled water Preparation. After activation, transfer to liquid 2216E medium, shake culture at 20°C to the exponential growth phase to obtain fermentation broth; centrifuge the fermentation broth, discard the precipitate, and store the supernatant at 4°C for later use; in the supernatant Add 1 / 2 volume of ethyl acetate for extraction, collect the aqueous phase, add 1 / 2 volume of ethyl acetate again for extraction, and collect the aqueous phase. The measured macroporous adsorption resin has an average pore diameter of 300mm. It is cleaned and activated according to the instructions and packed into the column. The measured macroporous adsorption resin is 1.7 times the aqueous solution on the column, and the amount used is 1.7 times the macroporous adsorption resin. The column was washed...

Embodiment 2

[0030] Similar to Example 1, the difference is that the 101X strain fermentation broth is prepared, and the deposited strain 101X uses MM medium: (NH4)2SO4 0.1%, K2HPO4 0.7%, KH2PO4 0.3%, sodium citrate 0.05%, MgSO4 0.01%, Prepared with 0.5% glucose, 1.5% agar and distilled water. After activation, transfer to liquid 2216E medium, shake culture at 15°C to the exponential growth phase to obtain fermentation broth; centrifuge the fermentation broth, discard the precipitate, and store the supernatant at 4°C for later use; in the supernatant Add 1 / 2 volume of ethyl acetate for extraction, collect the aqueous phase, add 1 / 2 volume of ethyl acetate again for extraction, and collect the aqueous phase. The measured macroporous adsorption resin has an average pore diameter of 280mm. It is cleaned and activated according to the instructions and packed into the column. The measured macroporous adsorption resin is 1.5 times the aqueous solution on the column, and the dosage is 2 times the ...

Embodiment 3

[0033] Similar to Example 1, the difference is that the 101X strain fermentation broth is prepared, and the deposited strain 101X uses MM medium: (NH4)2SO4 0.1%, K2HPO4 0.7%, KH2PO4 0.3%, sodium citrate 0.05%, MgSO4 0.01%, Prepared with 0.5% glucose, 1.8% agar and distilled water. After activation, transfer to liquid 2216E medium, shake culture at 30°C to the exponential growth phase to obtain fermentation broth; centrifuge the fermentation broth, discard the precipitate, and store the supernatant at 4°C for later use; in the supernatant Add 1 / 2 volume of ethyl acetate for extraction, collect the aqueous phase, add 1 / 2 volume of ethyl acetate again for extraction, and collect the aqueous phase. The measured macroporous adsorption resin has an average pore diameter of 330mm. It is cleaned and activated according to the instructions and packed into the column. The measured macroporous adsorption resin is twice as large as the aqueous solution on the column, and the dosage is 1 ti...

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PUM

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Abstract

The invention relates the preparing method of AIDS-resistent I compound. The compound is deep-sea bacterial strain. The deep-sea bacterial strain is 101X (Pseudoalteromonas sp.101X), and the preservation number is CCTCC NO: M205039. The method comprises the following steps: activating bacterial strain 101X with inorganic salt culture medium, getting fermentation liquor; centrifuging fermentation liquor, removing deposition, and getting supernatant fluid; adding acetic ester into supernatant fluid, extracting, collecting aqueous phase, weighing adsorption resin, cleaning, activating, column packing, cleaning column with pure water, eluting, collecting eluent, removing methanol, freeze drying, and getting product.

Description

Technical field [0001] The invention relates to a bacterium and a compound prepared therefrom, in particular to a Pseudoalteromonas sp. (Pseudoalteromonas sp.) and an anti-AIDS virus type I (HIV-1) compound prepared therefrom. Background technique [0002] AIDS is a fatal infectious disease caused by the human immunodeficiency virus (HIV). The prevention and treatment of AIDS has become one of the frontiers of contemporary science, especially the research on antiviral drugs. There are 16 kinds of anti-HIV drugs that have been approved for clinical use in the past 20 years, and about a hundred of them are undergoing preclinical research and clinical trials. High-efficiency antiretroviral therapy (HAART) has achieved good results in the treatment of AIDS. Due to the application of HAART, the mortality rate of AIDS in the United States has been decreasing year by year since 1998. However, HAART has problems such as drug resistance, large drug side effects, high cost, and inability ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/04A61P37/00
Inventor 曾润颖林昱
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION