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Anti AIDS 1 type virus compound preparation method

An anti-AIDS, compound technology, applied in the direction of drug combination, allergic diseases, bacteria, etc., to achieve the effect of simple operation method

Inactive Publication Date: 2006-12-27
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The biological resources in the deep sea have not yet been developed, and it is more likely to find new medicinally active compounds from the new strains. Therefore, the present invention provides a new deep-sea bacterium and a compound with anti-HIV type I preparation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Prepare the 101X strain fermentation broth, use the preserved strain 101X with MM medium: (NH4)2SO4 0.1%, K2HPO4 0.7%, KH2PO4 0.3%, sodium citrate 0.05%, MgSO4 0.01%, glucose 0.5%, agar 2.0%, distilled water preparation. After activation, it was transferred to liquid 2216E medium, and cultured with shaking at 20°C until it reached the exponential growth phase to obtain a fermentation broth; the fermentation broth was centrifuged, the precipitate was discarded, and the supernatant was stored at 4°C for later use; in the supernatant Add 1 / 2 volume of ethyl acetate to the liquid for extraction, collect the water phase, add 1 / 2 volume of ethyl acetate again for extraction, and collect the water phase. The average pore diameter of the measured macroporous adsorbent resin is 300 mm. After cleaning and activating according to the instructions, the column is packed, and the aqueous phase solution of 1.7 times of the measured macroporous adsorbent resin is loaded on the column, ...

Embodiment 2

[0030]Similar to Example 1, the difference is to prepare the 101X bacterial strain fermented liquid, the preserved bacterial strain 101X uses MM medium: (NH4)2SO4 0.1%, K2HPO4 0.7%, KH2PO4 0.3%, sodium citrate 0.05%, MgSO4 0.01%, Glucose 0.5%, agar 1.5%, distilled water preparation. After activation, it was transferred to liquid 2216E medium, and cultured with shaking at 15°C until it reached the exponential growth phase to obtain a fermentation broth; the fermentation broth was centrifuged, the precipitate was discarded, and the supernatant was stored at 4°C for later use; in the supernatant Add 1 / 2 volume of ethyl acetate to the liquid for extraction, collect the water phase, add 1 / 2 volume of ethyl acetate again for extraction, and collect the water phase. The average pore diameter of the measured macroporous adsorbent resin is 280mm. After cleaning and activating according to the instructions, the column is packed, and the aqueous phase solution of 1.5 times of the measure...

Embodiment 3

[0033] Similar to Example 1, the difference is to prepare the 101X bacterial strain fermented liquid, the preserved bacterial strain 101X uses MM medium: (NH4)2SO4 0.1%, K2HPO4 0.7%, KH2PO4 0.3%, sodium citrate 0.05%, MgSO4 0.01%, Glucose 0.5%, agar 1.8%, distilled water preparation. After activation, it was transferred to liquid 2216E medium, and cultured with shaking at 30°C until it reached the exponential growth phase to obtain a fermentation broth; the fermentation broth was centrifuged, the precipitate was discarded, and the supernatant was stored at 4°C for later use; in the supernatant Add 1 / 2 volume of ethyl acetate to the liquid for extraction, collect the water phase, add 1 / 2 volume of ethyl acetate again for extraction, and collect the water phase. The average pore diameter of the measured macroporous adsorbent resin is 330mm. After cleaning and activating according to the instructions, the column is packed, and the aqueous phase solution is twice as much as the me...

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PUM

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Abstract

The invention relates the preparing method of AIDS-resistent I compound. The compound is deep-sea bacterial strain. The deep-sea bacterial strain is 101X (Pseudoalteromonas sp.101X), and the preservation number is CCTCC NO: M205039. The method comprises the following steps: activating bacterial strain 101X with inorganic salt culture medium, getting fermentation liquor; centrifuging fermentation liquor, removing deposition, and getting supernatant fluid; adding acetic ester into supernatant fluid, extracting, collecting aqueous phase, weighing adsorption resin, cleaning, activating, column packing, cleaning column with pure water, eluting, collecting eluent, removing methanol, freeze drying, and getting product.

Description

technical field [0001] The invention relates to a bacterium and a compound prepared therefrom, in particular to a Pseudoalteromonas sp. and an anti-AIDS virus I (HIV-1) compound prepared therefrom. Background technique [0002] AIDS (AIDS) is a fatal infectious disease caused by human immunodeficiency virus (HIV). The prevention and treatment of AIDS has become one of the frontiers of contemporary science, especially promoting the research of antiviral drugs. There are 16 kinds of anti-HIV drugs that have been approved for clinical use in the past 20 years, and nearly a hundred varieties are undergoing preclinical research and clinical trials. Highly effective antiretroviral therapy (HAART) has achieved good results in the treatment of AIDS. Due to the application of HAART, the mortality rate of AIDS in the United States has been decreasing year by year since 1998. However, HAART has problems such as drug resistance, high drug side effects, high cost, and inability to clear...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04A61P37/00
Inventor 曾润颖林昱
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION