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Card, kit system in use for analyzing sample of liquid

A fluid sample and cartridge technology, applied in the field of cartridge systems for nucleic acid analysis, can solve problems such as retention and increase system cost, and achieve the effects of easy processing, low cost, and simple structure

Active Publication Date: 2011-12-28
CAPITALBIO CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the parts in direct contact with samples or reagents are not suitable for repeated use, and are limited by the current level of micro-processing, this integration method increases the cost of the system, making the integrated nucleic acid analysis system stay at the laboratory prototype stage, and it is difficult to form product enters the market

Method used

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  • Card, kit system in use for analyzing sample of liquid
  • Card, kit system in use for analyzing sample of liquid
  • Card, kit system in use for analyzing sample of liquid

Examples

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Embodiment Construction

[0028] The present invention follows the principle of separating the sample carrying part and the process control part as much as possible, and makes the former into the form of a cartridge on which a nucleic acid extraction unit, an acid amplification unit and a nucleic acid detection unit for nucleic acid analysis are integrated, Each unit is connected by micro-machined fluid pipelines, and the entire analysis process is controlled by an external control system independent of the cartridge. The control system at least includes a pump that can be started and stopped, a valve that can be switched on and off, and a detector for detecting the fluid position. They form a feedback system to manipulate the movement of the fluid sample on the cartridge. Among them, choosing a pump that can be started and stopped instead of a constant pressure air source as the driving force for fluid movement can easily obtain and remove the pressure difference required to push the fluid, avoiding c...

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Abstract

A check box system used for analyzing fluid sample is prepared for forming control component by a pump, a valve and a detector; using micro-processing technique to from out check box; setting a micro-pipeline in said box; connecting one end of micro-pipeline to pump and arranging a hole being used to flow in fluid sample between two ends of micro-pipeline.

Description

technical field [0001] The invention relates to a biological detection method and its special equipment, in particular to a cartridge system for nucleic acid analysis. Background technique [0002] With the development of biotechnology, the detection and analysis of nucleic acids are increasingly used in various detection targets, including microbial identification, personal identification, disease diagnosis, biological terrorism prevention, environmental protection and other aspects. However, the conventional nucleic acid analysis technology has many operation steps and is complicated to operate, which is prone to operational errors and cross-contamination. [0003] System integration and automation are effective ways to avoid contamination and inefficiency caused by human operations during nucleic acid analysis. An integrated nucleic acid analysis system can be functionally divided into two parts: sample loading and process control. The sample carrying part provides a ph...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N35/00C12Q1/68
CPCB01L2200/143B01L2300/0816B01L2200/10B01L2400/0487B01L3/50273B01L2300/0861B01L2300/0887B01L2400/0406B01L2300/0867B01L2200/0631B01L2300/1883C12Q2565/628B01L3/502707B01L2400/0688B01L3/502738B01L2200/147B01L3/502761B01L2300/1827B01L2300/0636B01L7/52
Inventor 郭旻官晓胜周骋翁东旭胡玉明张万杰郭永刚张冠斌程京
Owner CAPITALBIO CORP
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