Total triterpenes of solidago canadensis extracts, preparation method and use thereof

The technology of extract and total triterpenes is applied in the field of total triterpenes extract of the traditional Chinese medicine Solidago americana and its preparation field, which can solve the problem that the treatment of esophageal cancer only relies on surgery and radiotherapy, restricting market development, and the influence of liver function and transaminase index, etc. question

Inactive Publication Date: 2007-08-01
SHANGHAI HAIYI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0081] Tetrahydroaminoacridine is the first cholinesterase inhibitor approved by the FDA and clinically used to treat Alzheimer's disease, but regulations still need to be improved
[0088] The disadvantage of tacrine is that it has a great impact on liver function and transaminase index, which restricts the development of the market, and its sales growth is far behind other AD drugs
And because the vast majority of patients with esophageal cancer are already in the advanced stage when they are diagnosed, not only the local lesions have been extensively infiltrated, but also the distant spread of subclinical lesions or lymph node metastasis have been fo...

Method used

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  • Total triterpenes of solidago canadensis extracts, preparation method and use thereof
  • Total triterpenes of solidago canadensis extracts, preparation method and use thereof
  • Total triterpenes of solidago canadensis extracts, preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0373] One of the extraction methods of embodiment 1, Solidago americana total triterpene extract

[0374] Take 500 kg of Solidago americana medicinal material, add 5 tons of 70% ethanol, heat and reflux for extraction for 3 hours, filter, add 3 tons of ethanol for reflux and extract for 2 hours, filter, and combine the two extracts. The solvent is recovered under reduced pressure, the temperature is 50-60°C, concentrated to 8 times the volume of the original medicinal material, the concentrated solution is diluted with 3 times hot water, an appropriate amount of Chinese medicine clarifying agent is added, centrifuged, the precipitate is removed, the supernatant is taken, and the sample is placed on the large Porous resin column (pre-treat the resin column with acid, alkali, water, and alcohol before loading the sample), rinse with 3BV of pure water after loading, discard the aqueous solution, and use 3BV of 70% ethanol to elute. The ethanol-eluted plate was collected at a flo...

Embodiment 2

[0375] Embodiment 2, one of quality analysis methods of Solidago americana total triterpene extract

[0376] Samples used in mass analysis include:

[0377] Sample: according to the method of Example 1, extract the total triterpene extract of Solidago americana from Solidago americana, totally 5 batch numbers;

[0378] 1. Content analysis

[0379] 1.1 Determination of the content of total triterpenes

[0380] Vanillin-perchloric acid colorimetric method was used to determine the content of the total triterpenoid extract of Solidago americana, in which the ultraviolet spectrophotometer was UV-2401PC type, and the linear regression method of the standard curve was used, with Saponin 1 as the reference substance, in Measured at 590mm.

[0381] 1.1.1 Preparation of standard solution

[0382] Accurately weigh 30 mg of goldenrod saponin 1 powder, dissolve in methanol, and dilute to 5 ml. The concentration of the prepared reference substance solution is 6.1 mg / ml, accurately abs...

Embodiment 3

[0405] Example 3: In Vitro Antioxidative Pharmacological Effects of Solidago americana Total Triterpenes Extract

[0406] 1.1 The scavenging effect of the total triterpene extract of Solidago americana on superoxide anion free radicals

[0407] In 16mmol / L Tris-HCl buffer (pH8.0) containing nicotinamide adenine dinucleotide (NADH) 73μmol / L, phenazine methosulphate (PMS) 15μmol / L, nitrogen blue tetrazole ( nitroblue tetrazolium, NBT) 50μmol / L produces O 2- , PMS was not added to the blank tube, and the total triterpene extract of Solidago americana was not added to the control tube. The final concentration of the total triterpene extract of Solidago americana was 20 mg / L, 40 mg / L, 80 mg / L, 160 mg / L, and the total The volume is 3ml, the absorbance is measured at 560nm, and the inhibition rate is calculated. The results showed that the extract of total triterpenes from Solidago americana could significantly remove superoxide anion free radicals produced by the NADH-PMS-NBT syst...

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Abstract

The invention relates to the preparing method and application of the total triterpene extracts from American solidago decurense. The total triterpene glycosides in the total triterpene extracts include Solidago decurense saponin 1,2,3 and 4, which can prevent and/or treat esophageal cancer, senile dementia, respiratory tract disease caused by different kinds of inflammation such as laryngopharyngitis and tracheitis. The diseases mentioned has been the main pathogeny that influences the quality of human life. The preparing technology of the extract is very simple, the extracting efficiency is very high; the extracts have strong pharmacologic action, stable quality and controllable quality; it's safe with less toxicity. The American Solidago decurense has damaged Chinese natural resources and ecological environment seriously as foreign invasive plant, it has been removed as weeds in many places. The invention can change waste into treasure while providing a new medicine source for preventing, diagnosing, detecting, protecting, treating and researching the mentioned diseases.

Description

technical field [0001] The present invention relates to the technical fields of medicine, food and beverage, in particular to a traditional Chinese medicine extract and its preparation method and application, more specifically to a total triterpene extract of the traditional Chinese medicine Solidago americana and its preparation method and application . Background technique [0002] (1) Research overview of Solidago americana [0003] 1 Overview [0004] Solidago americana, also known as solidago canadensis, is the whole plant, root or rooted whole plant of solidago canadensis (Solidago canadensis) in the Compositae plant. Solidago americana was first recorded in the "Plant Names and Facts" mountain grasses, and it is mainly distributed in East China, Central South, Shaanxi, Taiwan and other places. [0005] Solidago is a genus in the family Compositae. There are about 120 species of Solidago genus in the world, and 4 species are distributed in China, namely Solidago de...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61P39/06A61P29/00A61P35/00
Inventor 郑水庆郑榕秦路平
Owner SHANGHAI HAIYI BIO TECH
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