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Isolation and purification method of pueraria flavones using magnetic adsorbent resin and magnetic field

A technology of kudzu root flavonoids and external magnetic field, which is applied in selective adsorption, ion exchange, organic chemistry, etc., to achieve the effects of excellent service life, improved separation and purification efficiency, and increased contact area

Inactive Publication Date: 2007-08-01
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no method for separating and purifying kudzu flavonoids by using magnetic adsorption resin and external magnetic field

Method used

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  • Isolation and purification method of pueraria flavones using magnetic adsorbent resin and magnetic field

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] According to the process flow shown in Figure 2, the specific operation steps for separating and purifying kudzu flavonoids using magnetic adsorption resin and external magnetic field are as follows:

[0030] (1) Microwave extraction of pueraria flavonoids extract: put 5.000 g pueraria dry powder with a crushing degree of 80 mesh in a microwave extraction tank, add 95% ethanol according to the solid-to-liquid ratio of 1:8, and extract for 8 minutes at a microwave power of 700W . After the extraction is completed, filter and remove impurities to obtain a yellow-brown Pueraria flavonoids extract with an extraction rate of 51.8%.

[0031] (2) Separation and purification of Pueraria flavonoids by magnetic adsorption resin: put 5.000g of magnetic adsorption resin into a Φ15mm×300mm resin column, wash with ethanol until the effluent is not turbid after adding water, and then wash with water until the effluent does not contain ethanol. The resin column was placed in an adjust...

Embodiment 2

[0033] According to the process flow shown in Figure 2, the specific operation steps for separating and purifying kudzu flavonoids using magnetic adsorption resin and external magnetic field are as follows:

[0034] (1) Microwave extraction of pueraria flavonoids extract: put 5.000 g pueraria dry powder with a crushing degree of 80 mesh in a microwave extraction tank, add 95% ethanol according to a solid-to-liquid ratio of 1:5, and extract for 5 minutes at a microwave power of 1000W . After the extraction is completed, filter and remove impurities to obtain a yellow-brown pueraria flavonoid extract with an extraction rate of 49.8%.

[0035] (2) Separation and purification of Pueraria flavonoids by magnetic adsorption resin: put 5.000g of magnetic adsorption resin into a Φ15mm×300mm resin column, wash with ethanol until the effluent is not turbid after adding water, and then wash with water until the effluent does not contain ethanol. The resin column was placed in an adjustab...

Embodiment 3

[0037] According to the process flow shown in Figure 2, the specific operation steps for the separation and purification of kudzu flavonoids by using magnetic adsorption resin and external magnetic field are as follows:

[0038](1) Microwave extraction of pueraria flavonoids extract: put 5.000 g pueraria dry powder with a crushing degree of 80 mesh in a microwave extraction tank, add 95% ethanol according to a solid-to-liquid ratio of 1:10, and extract for 10 minutes at a microwave power of 700W . After the extraction is completed, filter and remove impurities to obtain a yellow-brown Pueraria flavonoids extract with an extraction rate of 45.2%.

[0039] (2) Separation and purification of Pueraria flavonoids by magnetic adsorption resin: put 5.000g of magnetic adsorption resin into a Φ15mm×300mm resin column, wash with ethanol until the effluent is not turbid after adding water, and then wash with water until the effluent does not contain ethanol. The resin column was placed ...

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Abstract

There's provided a way to separate and purify kudzuvine root anthoxanthin with magnetic adsorbing resin and external magnetic field. Firstly, putting kudzuvine root into the micro wave extracting jar, charging alcohol into the jar to remove impurities and get the extracted solution of kudzuvine root anthoxanthin; putting magnetic adsorbing resin into the resin column and putting it in the adjustable magnetic field, letting the extracted solution go through the resin and collecting the solution; condensing and drying to get the product. The advantages of the invention: it's provided a easy and cheap way to separate the kudzuvine root anthoxanthin; the replacement of magnetic resin for common resin has not only maintained the adsorbing ability of resin, but also showed better capabilities in to-be-treated material amount, density of extracted solution, volume of extracted solution and utilizing cycle; the enforcement of the external magnetic field has improved the adsorbing and restoring ability of the resin. The adsorbing rate of kudzuvine root anthoxanthin is larger than 88% in the invention.

Description

technical field [0001] The invention relates to a method for separating and purifying Pueraria flavonoids, in particular to a method for separating and purifying Pueraria flavonoids by using a magnetic adsorption resin and an external magnetic field. Background technique [0002] The research and development of active ingredients of natural medicines is an important means for human beings to find new medicines. With the continuous development of modern medicine, food and other industries, the emphasis on Chinese herbal medicine is increasing at home and abroad. As a medicinal plant, kudzu root has unique biological activity, and its research, development and utilization have very broad prospects. [0003] The isoflavones contained in kudzu root are its main physiologically active components. Pueraria flavonoids have the effects of lowering blood pressure, slowing heart rate, dilating coronary arteries, reducing myocardial oxygen consumption, impro...

Claims

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Application Information

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IPC IPC(8): A61K36/48B01D15/08C07D311/30C07H17/07A61K125/00
Inventor 李冰赵巍李琳黎锡流陈玲张喜梅胡松青李晓玺
Owner SOUTH CHINA UNIV OF TECH
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