Dolphin streptococcal white-oil adjuvant inactivated vaccine and preparing method
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A technology of Streptococcus iniae and inactivated vaccine, applied in antibacterial drugs, pharmaceutical formulations, bacterial antigen components, etc., can solve the problem of high cost of adjuvants, no reports on the research and development of fish Streptococcus vaccines, and difficulty in popularization and application, etc. problem, to achieve the effect of good control effect
Inactive Publication Date: 2007-08-29
SUN YAT SEN UNIV
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However, this adjuvant has the following disadvantages: 1. it causes local tissue damage and affects the meat quality; 2. complete Freund's adjuvant contains mycobacteria, which makes the tuberculin test positive; Promote and apply in practice
So far, there have been no reports on the research and development of fish streptococcal vaccines in my country
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Embodiment 1
[0020] 1. Recovery and expansion of frozen strains
[0021] Streak the standard strains HD-1 and TBY-1 of frozen-preserved virulent strains stored at -70°C and BHI solid medium for 48 hours at 28°C (see Figure 1), and inoculate a single colony in 10 mL of liquid BHI liquid medium , shake culture at 28°C for 48h; then inoculate all the cultures in a large dose of BHI liquid medium, and shake at 28°C for 48h. The cultures were then serially diluted plated and counted. Figure 3 is the picture of Gram-stained cells of Streptococcus iniae. See Table 1 for the physiological and biochemical tests of the virulent strain standard strains HD-1 and TBY-1.
[0022] Table 1 Streptococcus iniae HD-1, TBY-1 physiological and biochemical test results
[0023] Standard strain
(ATCC29178)
HD-1
TBY-1
to grow
10°C
6.5% NaCl
45°C
hemolysis
athleticism
-
-
-
N
-
-
- ...
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Abstract
The present invention relates to a dolphin streptococcus white oil adjuvant inactivated vaccine and its preparation method. Said preparation method includes the following steps: (1), separating out dolphin streptococcus from sea-water cultivated or fresh-water cultivated diseased dolphin; (2), making the above-mentioned obtained dolphin streptococcus undergo the processes of expansive cultivation, inactivation and concentration so as to obtain the concentrated inactivated streptomoccus liquor; (3), mixing 96% of the inactivated concentrated streptococcus liquor and 4% of steril Tween-80 and shaking to make Tween-80 be completely dissolved, and using their mixture as aqueous phase; mixing 94% of No.10 white oil and 6% of Span-80, adding 2% of aluminium stearate into the above-mentioned mixture solution and using said mixture solution as stabilizing agent, uniformly mixing and stirring them, heating to obtain a transparent material, making said transparent material undergo the process of high-pressure sterilization treatment and using it as oily phase; according to the ratio of aqueous phase: oily phase=3:7 high-speed stirring and emulsifying so as to obtain the invented product. Said vaccine can obtain good effect for preventing and curing dolphin streptococcicosis.
Description
technical field [0001] The invention relates to an inactivated vaccine, in particular to an inactivated vaccine with white oil adjuvant of Streptococcus iniae and a preparation method thereof. Background technique [0002] Streptococcus iniae, belonging to the genus Streptococcus of the family Streptococcus, is round or oval, in pairs or chains of different lengths, without flagella, immobile, encapsulated, non-spore-forming, Gram Positive staining, facultative anaerobic. S. iniae was originally isolated and named by Pier (1976) from a subcutaneous abscess of an Amazonian freshwater dolphin. In 1997, Weinstein et al. proved that S.iniae is a conditional zoonotic pathogen, which can not only infect almost all sea and freshwater fish, such as tilapia, perch, sea bream, grouper, salmon, rainbow trout etc., can also infect humans, causing cellulitis and endocarditis. [0003] After S. iniae infects susceptible fish species such as tilapia, barramundi, and striped hybrid, it c...
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