Method for measuring human blood serum cetylic acid and oleic acid concentration ratio with gas phase chromatography and application thereof

A technology of gas chromatography and gas chromatograph, which is applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of lack and difficulty in early diagnosis, and achieve the effect of accurate prevention and diagnosis, early diagnosis and auxiliary diagnosis.

Inactive Publication Date: 2007-10-31
NANJING UNIV
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Problems solved by technology

[0005] CAD has developed to a certain extent, especially when there are obvious organ lesions, it is not difficult to diagnose, but early diagnosis is not easy, and there is a lack of targeted diagnosis of susceptible high-risk groups such as obese people

Method used

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  • Method for measuring human blood serum cetylic acid and oleic acid concentration ratio with gas phase chromatography and application thereof
  • Method for measuring human blood serum cetylic acid and oleic acid concentration ratio with gas phase chromatography and application thereof
  • Method for measuring human blood serum cetylic acid and oleic acid concentration ratio with gas phase chromatography and application thereof

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Embodiment Construction

[0024] The present invention will be further described below in conjunction with specific examples, but there is no limitation thereto.

[0025] Sample collection: Serum samples were provided by the Department of Cardiothoracic Surgery, Drum Tower Hospital, Affiliated Hospital of Nanjing University.

[0026] Materials and Reagents:

[0027] Reagents: Chromatographically pure palmitic acid and oleic acid were purchased from Sigma Corporation in the United States, analytically pure concentrated sulfuric acid was purchased from Analytical pure methanol, analytically pure chloroform and anhydrous sodium sulfate were purchased from Tianjin No. 1 Chemical Reagent Factory.

[0028] Equipment: water bath, votex, centrifuge, gas chromatograph HP-6890 (Agilent, USA).

[0029] 1 Preparation of standard products and acquisition of human serum samples

[0030] Preparation of standard products: use chromatographically pure palmitic acid and oleic acid to prepare and dissolve in methanol a...

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Abstract

This invention belongs to biological medical technique field and to one gas phase spectrum test method for human serum cetylic acid and oleic acid concentration proportion and the application for fat person coronary artery atherosclerosis cardiology rapid diagnose, which comprises the following steps as: processing serum sample derive to test the above substance concentration proportion and then test the atherosclerosis disease.

Description

1. Technical field [0001] The invention belongs to the technical field of biomedicine, in particular to a method for measuring the concentration ratio of palmitic acid (PA) and oleic acid (OA) in human serum by gas chromatography and in the diagnosis of coronary atherosclerotic heart disease (CAD) in obese people Applications. 2. Background technology [0002] Atherosclerosis refers to the occurrence of non-inflammatory, degenerative and proliferative lesions in arteries, resulting in thickening and hardening of the vessel wall, loss of elasticity and narrowing of the lumen. The pathological changes of atherosclerosis mainly involve large elastic arteries (such as the aorta) and medium-sized muscular elastic arteries (coronary arteries and cerebral arteries are most affected) in the systemic circulation system. The lesions of the affected arteries start from the intima, and there are several These lesions exist at the same time, including local accumulation of lipids and co...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
Inventor 张燕王强张辰宇项阳孙凌冰徐威姜晓宏王东进
Owner NANJING UNIV
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