Use of red nocardial cell wall skeleton in preparation of medicine

A technology of Nocardia rubrum and cell wall, which is applied in the field of preparation of drugs for the treatment of cervical precancerous lesions, can solve the problems of exaggerating the nature of cervical precancerous lesions, over-treating patients, and treatment confusion, so as to avoid adverse reactions in the body, ensure curative effect, receptive effect

Inactive Publication Date: 2007-11-21
LIAONING GREATEST BIO-PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] At present, in the treatment of cervical precancerous lesions in China, the treatment is relatively chaotic. Due to the fear of cancer, it is very common to treat patients based solely on the results of cervical cytology smears, exaggerate the nature of cervical precancerous lesions, and over-treat patients.

Method used

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  • Use of red nocardial cell wall skeleton in preparation of medicine
  • Use of red nocardial cell wall skeleton in preparation of medicine
  • Use of red nocardial cell wall skeleton in preparation of medicine

Examples

Experimental program
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Effect test

experiment example

[0039] 1. Detection of the inhibitory effect of antineoplastic drug-Nocardia rubrum cell wall skeleton preparation on cervical cancer cells

[0040] method:

[0041] MTT (tetramethylazolyl blue) method was used to detect cell viability, and the cell growth curve was drawn, and the experimental results were analyzed with statistical software.

[0042] (1) Main materials and reagents

[0043] DMEM medium (Gibco, USA), trypsin (Sigma, USA), MTT (Sigma, USA), fetal bovine serum (Hyclone, USA), dimethyl sulfoxide (Sigma, USA), 96 wells Plate (Corning Corporation)

[0044] (2) Preparation of MTT

[0045] Prepare a 5 mg / ml stock solution with PBS, filter and sterilize with a 0.22 μm filter membrane, and store at 4°C in the dark for one week.

[0046] (3) Preparation of Nocardia rubra cell wall skeleton preparation

[0047] Nr-cws was provided by Shenyang Shengbaokang Biopharmaceutical Co., Ltd. After the collected Nr-cws was configured into a suspension with a certain concentrat...

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Abstract

The invention is concerned with the drug for treating cervical intraepithelial neoplasia which prepare by Nocadia rubra Cell Wall Skeleton.

Description

technical field [0001] The invention relates to the application of the Nocardia rubra cell wall skeleton in the preparation of medicines, especially the application in the preparation of medicines for treating cervical precancerous lesions. Background technique [0002] Precancerous lesions of the cervix are clinically difficult gynecological diseases, which are self-limiting and malignant. It takes about 10 years for the transition period to develop into cervical cancer. Since cervical precancerous lesions are a gradual and long process, it also provides sufficient time and conditions for early detection and early treatment. In particular, patients with mild cervical precancerous lesions are more likely to be cured. [0003] Cervical precancerous lesions are commonly referred to as cervical squamous intraepithelial lesions (SZL) or cervical intraepithelial neoplasia (CIN). It is also subdivided into atypical squamous cell (ASCUS) and atypical glandular cell (AGUS), and l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A61K9/00A61P35/00A61P31/12
CPCA61K9/0014A61K35/74A61P31/12A61P35/00
Inventor 张策张轶洪晓明张国英赵健
Owner LIAONING GREATEST BIO-PHARM CO LTD
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