Method for constructing composite type presenile dementia animal model

A technology of Alzheimer's disease and animal models, which is applied in animal husbandry and other fields, can solve the problems of non-replication and difficult operation, and achieve the effect of low cost, good reproducibility and simple operation

Inactive Publication Date: 2008-01-23
华中科技大学同济医学院
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

In addition, methods (2) and (3) both require intracerebral injection, which is difficult to operate; none of the three methods can replicate animal models that simultaneously exhibit tau, A-beta, and behavioral abnormalities

Method used

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  • Method for constructing composite type presenile dementia animal model
  • Method for constructing composite type presenile dementia animal model
  • Method for constructing composite type presenile dementia animal model

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0039] Example 1 Tail vein injection of homocysteine ​​(Hcy) to construct AD-like rat animal model

[0040] (1) Experimental animals and groups

[0041] Male Wistar rats (provided by the Experimental Animal Center of Tongji Medical College, Huazhong University of Science and Technology), SPF grade, weighing 250-320 grams, 90 rats, were reared in a conventional environment. Divided into 6 groups, 15 rats in each group: ①Normal group; ②Sham operation group; ③Hcy group 1 (100 μM); ④Hcy group 2 (350 μM); ⑤Hcy group 1+folate / VitB12; ⑥Hcy group 2+folate / VitB12.

[0042] (2) Main reagents

[0043] Acrylamide (Arc), N,N-Methylenebisacryloyl (bis), Tetramethylethylenediamine (TEMED), Sodium Dodecyl Sulfate (SDS), Glycine (Glycine), Bovine Serum Albumin (BSA), Tris (Tris), Sodium Vulcanate (Na3 VO4), β-Glycerol Phosphate (β-PG), Sodium Fluoride (NaF), Hcy is a Sigma product; Ammonium Persulfate (AP) and Coomassie Brilliant Blue Protein Chromogenic Solution were purchased from Pierce ...

example 2

[0071] Example 2 intraperitoneal injection of homocysteine ​​(Hcy) to construct AD-like rat animal model

[0072] Except that the route of injection is different, other operations and results are the same as Example 1

[0073] Conclusion: By intraperitoneal injection of 1ml (100μM) homocysteine ​​in rats for 14 days, an animal model that simultaneously exhibits learning and memory impairment, formation of a large number of senile plaques and abnormal phosphorylation of tau was successfully constructed.

example 3

[0074] Example 3 lateral ventricle injection of homocysteine ​​(Hcy) to construct AD-like rat animal model

[0075] (1) The evaluation of experimental animals, groups, reagents, equipment and models is the same as in Example 1

[0076] (2) Injection into the lateral ventricle

[0077] After the rat was anesthetized, it was fixed on the brain stereotaxic instrument, and the anterior and posterior fontanelles were clearly exposed. The needle was inserted vertically at 1.0-1.2 mm behind the anterior bregma, 1.5-1.8 mm lateral to the midline to about 3.5-3.8 mm under the dura mater, and the needle was fixed. Slowly inject 20 μl (100 μM) of Hcy liquid, once a day, for 14 days.

[0078] (3) The evaluation of the model is the same as Example 1

[0079] Conclusion: By intraperitoneal injection of 1ml (100μM) homocysteine ​​in rats for 14 days, an animal model that simultaneously exhibits learning and memory impairment, a large number of senile plaques and abnormal phosphorylation of...

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Abstract

The invention provides a method for constituting presenile dementia bandicoot model by injecting Hcy to caudal vein, abdomen or ventriculus laterali cerebri of the bandicoot, which can induce the brain mantle and hippocampal cortex amyloid of the bandicoot to deposit to generate age pigment and lead tau undertake exceeding abnormal phosphorylation as well as appear compound symptom like academic handicap and dysmnesia, etc. The model can be used for defending presenile dementia, screening new medicines, appraising the curing effect and researching the nosogenesis.

Description

technical field [0001] The invention relates to medical evaluation and detection technology, in particular to a method for constructing an animal model for screening drugs, researching disease occurrence mechanism and evaluating drug curative effect. Background technique [0002] Alzheimer's disease (AD) is a common neurodegenerative disease, its main symptoms are memory loss, low cognitive ability and slow thinking, and progressive aggravation, finally unable to take care of itself and die, the course of the disease is generally 8 ~10 years. With the acceleration of the aging process of the society, the number of patients with AD has risen sharply, and it has become a serious disease that threatens the physical and mental health of the elderly, especially the elderly population, and has brought serious social, economic and family problems. [0003] The main pathological changes in the brain of AD patients are the formation of a large number of neurofibrillary tangles (neur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/00
Inventor 王建枝
Owner 华中科技大学同济医学院
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