Method for detecting animalcule in chinese medicinal materials with AOTF near-infrared spectrometer

A near-infrared spectrometer and near-infrared spectroscopy technology, applied in biochemical equipment and methods, microbial measurement/inspection, color/spectral characteristic measurement, etc., can solve problems such as complex operation, smooth production process, and inability to maintain, and achieve The effect of fast detection speed, high accuracy, and no consumption of reagents

Active Publication Date: 2008-02-06
XIAMEN TRADITIONAL CHINESE MEDICINE +1
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Problems solved by technology

[0002] At present, in the pharmaceutical industry of traditional Chinese medicine, the detection of bacteria and other microorganisms is a necessary test item in accordance with the provisions of the National Pharmacopoeia. The conventional detect

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  • Method for detecting animalcule in chinese medicinal materials with AOTF near-infrared spectrometer
  • Method for detecting animalcule in chinese medicinal materials with AOTF near-infrared spectrometer
  • Method for detecting animalcule in chinese medicinal materials with AOTF near-infrared spectrometer

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Embodiment Construction

[0040] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0041] Instrument conditions and sample handling:

[0042] Instrument: Luminar 5030 portable AOTF technology near-infrared spectrometer produced by BRIMROSE Company in the United States. The main components include: optical part, control part, power adapter and notebook computer. The wavelength range of the instrument is 1100nm to 2300nm, the wavelength increment is 2nm, the number of scans is 300, and an InGaAs detector is used. Norwegian CAMO company TheUnscrambler analysis software.

[0043] Samples: 20 irregular granular samples of Xinhuang Tablets, numbered 1-20, of which the microorganisms in samples No. 1 and No. 2 have been killed and are inactive bacteria, and the microorganisms in samples No. 3-20 are active bacteria body. And provide the data of the number of bacteria and molds for each sample, the unit is: piece / gram.

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Abstract

The present invention relates to a method to detect microorganism in Chinese traditional medicine by using an AOTF approximate infrared spectrometer, and comprises the following steps: firstly, the spectral datum of a correcting set sample are collected by using the AOTF approximate infrared spectrometer. Secondly, a qualitative correcting model is founded according to the collected spectral datum. Thirdly, a quantitative correcting model is founded according to the correlation between the spectral datum and the microbial datum. Fourthly, the approximate infrared spectral datum of an unknown model are collected. Fifthly, the qualitative and quantitative analysis results about the microorganism are obtained through transferring the correcting model. The AOTF-NIR technology is used in the present invention to realize the fast detecting work of the lab microorganism. At the same time, the fast detection of microorganism can be realized online and the activity of the microorganism can be easily identified. The present invention has the advantages of no need of pretreatment of the sample, fast detecting speed (second grade speed), no consumption of reagent, green environmental protection analysis without pollution and high accuracy.

Description

technical field [0001] The invention relates to a method for detecting microorganisms, in particular to a method for detecting microorganisms in traditional Chinese medicine by using an AOTF near-infrared spectrometer. Background technique [0002] At present, in the pharmaceutical industry of traditional Chinese medicine, the detection of bacteria and other microorganisms is a necessary test item in accordance with the provisions of the National Pharmacopoeia. The conventional detection method is to use the medium culture and counting method for detection. This method is complicated, time-consuming and labor-intensive. , It takes at least 48 hours to get the final test results, which cannot maintain a smooth production process. Contents of the invention [0003] In order to overcome the above-mentioned deficiencies in the prior art, the present invention provides a method that does not require pretreatment of samples, has a fast detection speed (second-level speed), does ...

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Application Information

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IPC IPC(8): G01N21/35G01N21/27G06F17/00C12Q1/02G01N21/359
Inventor 蔡绍松墙世发洪澎雄包侠萍董海平陈雷邹振民谢川李腾
Owner XIAMEN TRADITIONAL CHINESE MEDICINE
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