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Subclinical ketosis of milk cattle diagnose indicator paper

A technology of test strips and ketosis, applied in the direction of biological testing, biochemical equipment and methods, material inspection products, etc., can solve the problems of long time-consuming, cumbersome operation, and high testing cost

Inactive Publication Date: 2008-02-06
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention provides a dairy cow subclinical ketosis diagnostic test paper to solve the problems of cumbersome operation, high detection cost, long time-consuming, low sensitivity and specificity in the detection process of dairy cow subclinical ketosis

Method used

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  • Subclinical ketosis of milk cattle diagnose indicator paper
  • Subclinical ketosis of milk cattle diagnose indicator paper
  • Subclinical ketosis of milk cattle diagnose indicator paper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The making of embodiment 1 test paper

[0041] Including a support pad 2 and a test pad 1 fixed thereon, the test pad of the test paper includes the following reagents:

[0042] D-(-)-3-hydroxybutyrate dehydrogenase, 2270U / mg, 50mg;

[0043] Nicotinamide adenine dinucleotide, 300mg;

[0044] Chromogenic agent is NBT, 100mg;

[0045] Diaphorase, 35U / mg, 500mg;

[0046] Tris Base, molecular weight 121.14, 1.21g;

[0047] Oxalic acid, molecular weight 126, 126mg;

[0048] Trehalose, molecular weight 383.3, 4g;

[0049] Distilled water 100ml.

[0050] Prepare 100mL reaction solution according to the formula, adjust the pH value to 8.5 with 1M hydrochloric acid, take thin medium-speed quantitative filter paper, completely immerse in the solution, take it out after 2 minutes, hang and dry in a constant temperature drying oven at 37°C for 45 minutes, and then The filter paper was cut into 3×3cM sheets, pasted on a 3×6cM polyethylene plastic sheet, put into a brown bottl...

Embodiment 2

[0051] The making of embodiment 2 test paper

[0052] D-(-)-3-hydroxybutyrate dehydrogenase, 2270U / mg, 75mg;

[0053] Nicotinamide adenine dinucleotide, 400mg;

[0054] Chromogenic agent is NBT, 150mg;

[0055] Diaphorase, 38U / mg, 750mg;

[0056] Tris Base, molecular weight 121.14, 1.21g;

[0057] Oxalic acid, molecular weight 126, 126mg;

[0058] Trehalose, molecular weight 383.3, 5g;

[0059] Distilled water 100ml.

[0060] The preparation method is the same as in Example 1.

Embodiment 3

[0061] The making of embodiment 3 test paper

[0062] D-(-)-3-hydroxybutyrate dehydrogenase, 2270U / mg, 100mg;

[0063] Nicotinamide adenine dinucleotide, 500mg;

[0064] Chromogenic agent is NBT, 200mg;

[0065] Diaphorase, 45U / mg, 1000mg;

[0066] Tris Base, molecular weight 121.14, 1.21g;

[0067] Oxalic acid, molecular weight 126, 126mg;

[0068] Trehalose, molecular weight 383.3, 6g;

[0069] Distilled water 100ml.

[0070] The preparation method is the same as in Example 1.

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Abstract

The present invention relates to a dairy cow sub-clinical ketosis diagnosis test paper which belongs to the art of the veterinary clinical laboratory science. The present invention comprises a support cushion and a test cushion that is fixed on the support cushion; and the present invention is characterized in that the test cushion of the test paper comprises the following reagents: D-(-)-3-Hydroxybutyrate Dehydrogenase with 2270U / mg and 50mg-100mg; nicotinamide adenine dinucleotide with 300mg-500mg; diaphorase with 35U / mg-45U / mg and 500-1,000mg; NBT with 100mg-200mg; Tris Base with molecular weight 121.14 and 1.21g; oxalic acid with molecular 126 and 126mg; fucose with molecular 383 and 4-6g; and distilled water with 100ml. The dairy cow sub-clinical ketosis diagnosis test paper of the present invention is used conveniently and has the advantages of economic practicality, high sensitivity and specificity, and good stability. The test paper is used to test D-(-)-3-Hydroxybutyrate and D-(-)-3-Hydroxybutyrate sodium in the milk of the milch cow.

Description

technical field [0001] The invention belongs to the field of veterinary clinical laboratory science, and the detected substance is D-(-)-3-hydroxybutyric acid and its salt in cow milk, which is suitable for monitoring subclinical ketosis of dairy cows in intensive dairy farms. Background technique [0002] Ketone bodies are the products of fatty acid metabolism, and the production of ketone bodies reflects the intensity of fat oxidation metabolism. Under normal circumstances, the concentration of ketone bodies in the blood is relatively constant, because the ketone bodies produced in the liver can be quickly utilized in extrahepatic tissues, especially the kidney and myocardium have a strong enzyme system that oxidizes acetoacetate, followed by the brain. Muscle tissue is also an important tissue for utilizing ketone bodies. The use of ketone bodies as an energy source is of great significance for brain tissue that cannot utilize fatty acids. However, under certain physiol...

Claims

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Application Information

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IPC IPC(8): G01N33/52G01N21/78C12Q1/00
Inventor 张志刚王哲李小兵刘国文张才逄晓阳张加力宋文学张乃生周昌芳
Owner JILIN UNIV
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