Ammonia diagnosis/determination reagent kit and ammonia concentration determination method

A concentration determination and kit technology, which is applied in the field of ammonia diagnosis/determination kits and ammonia concentration determination, can solve the problem of undiscovered blood ammonia determination methods, etc.

Inactive Publication Date: 2008-04-30
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Searching Chinese patents, only found 87105593.7 patent application which discloses a quick-frozen cup for measuring blood ammonia, but did not find an ideal method for measuring blood ammonia

Method used

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  • Ammonia diagnosis/determination reagent kit and ammonia concentration determination method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The ammonia diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0040] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0041] Stabilizer 50mmol / L

[0042] Coenzyme 3mmol / L

[0043] Glutamine Synthetase 4000U / L

[0044] Glyceraldehyde-3-phosphate dehydrogenase 6000U / L

[0045] Glutamic acid 16mmol / L

[0046] Adenosine triphosphate 6mmol / L

[0047] Glyceraldehyde-3-phosphate 4mmol / L

[0048] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0049] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the tested ammonia sample to the reagent is 1 / 25, the reaction The direction is positive reaction (rising reaction), the delay time is about 0 mi...

Embodiment 2

[0052] The ammonia diagnosis / measurement reagent of the present embodiment is a double reagent, comprising:

[0053] Reagent 1

[0054] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0055] Stabilizer 50mmol / L

[0056] Coenzyme 3mmol / L

[0057] Glutamic acid 12mmol / L

[0058] Adenosine triphosphate 8mmol / L

[0059] Glyceraldehyde-3-phosphate 6mmol / L

[0060] Reagent 2

[0061] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0062] Stabilizer 50mmol / L

[0063] Glutamine Synthetase 6000U / L

[0064] Glyceraldehyde-3-phosphate dehydrogenase 8000U / L

[0065]After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0066] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the tested ammonia sample to reagen...

Embodiment 3

[0069] The ammonia diagnosis / measurement reagent of the present embodiment is three reagents, comprising:

[0070] Reagent 1

[0071] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0072] Stabilizer 50mmol / L

[0073] Coenzyme 3mmol / L

[0074] Glutamic acid 20mmol / L

[0075] Reagent 2

[0076] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0077] Stabilizer 50mmol / L

[0078] Adenosine triphosphate 4mmol / L

[0079] Glyceraldehyde-3-phosphate 8mmol / L

[0080] Reagent 3

[0081] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0082] Stabilizer 50mmol / L

[0083] Glutamine Synthetase 8000U / L

[0084] Glyceraldehyde-3-phosphate dehydrogenase 4000U / L

[0085] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0086] When measuring ammonia concentration, set on the automatic biochemical analyzer: temperature 37°C, react...

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Abstract

The invention relates to an ammonia diagnosing/measuring reagent box which utilizes the technique of the enzyme contrasting color method and the enzyme jointing method, and belongs to the technical field of the medicine/food/environment test. The main components of the reagent box of the invention mainly include cushion liquid, coenzyme, glutamic acid, adentosine triphosphoric acid, glyceraldehyde-3-phosphate, glutamine synthetase, glyceraldehyde-3-phosphate dehydrogenase and stabilizing agent; the reagent box generates a series of enzymic reactions through the mixing of the samples and the reagent at a certain cubage rate, and then the reactants are positioned under an ultraviolet/visible light analyzer which tests the reducing speed of the absorbency at the main wave length of 340nm, thereby measuring the concentration size of the enzyme. The invention can absolutely get needed measuring result through the ultraviolet/visible light analyzer.

Description

technical field [0001] The invention relates to an ammonia diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring ammonia concentration, which belongs to the technical field of medicine / food / environmental inspection and measurement. Background technique [0002] Ammonia determination methods include micro-diffusion method, ion exchange method, enzymatic method and ammonia electrode method. The most widely used methods are enzymatic method and blood ammonia analyzer analysis method based on ion selective electrode. [0003] The diffusion method is that after the sample is alkalized, NH is released 3 , Titrate the released ammonia with acid, or use the Nessler reaction to form brown-yellow mercuric ammonium iodide for colorimetry. These methods need to be alkalized, and the endogenous ammonia formation will affect the accuracy and precision, so they are rarely used at present; the ion exchange method is more accurate than the di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N21/78G01N21/25C12Q1/32C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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