Stereoisomerically enriched 3-aminocarbonyl bicycloheptene pyrimidinediamine compounds and their uses
A compound, diastereomer technology, applied in the field of stereoisomer-enriched compositions, can solve problems such as limiting the effectiveness of chemotherapeutic agents
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[0199] The invention is further illustrated by reference to the following examples, which describe the preparation of various compounds described herein, methods for assaying their biological activity, and methods for their use. Many modifications in materials and methods of practice will be apparent to those skilled in the art without departing from the scope of the invention.
[0200] Preparation of 4-(4-methylpiperazin-1-yl)-3-methylnitrobenzene
[0201] reaction:
[0202]
[0203] Procedure: Homogenous mixture of 4-fluoro-3-methylnitrobenzene 1 (20 g, 129 mmol) and N-methylpiperazine 3 (25.82 g, 258 mmol) in N-methylpyrrolidone (NMP) (10 mL) in N 2 Reflux (120° C.) for 24 hours. The reaction mixture was cooled to room temperature and poured into saturated NaCl solution (100 mL). The resulting solid was sonicated for approximately 30 seconds, filtered, washed with ice-cold water (2 x 10 mL), and dried under high vacuum to yield 4-(4-methylpiperazin-1-yl)-3-methyl N...
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