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Process for separating extracting spherosinin from gansu whin

A technology of swainsonine and a process method, which is applied in the field of separation and extraction of swainsonine, can solve the problems of high cost, complicated extraction process, incapability of industrialized mass production, etc., and achieves the advantages of reducing intermediate links, simple process and low extraction cost. Effect

Active Publication Date: 2008-05-14
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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AI Technical Summary

Problems solved by technology

It is expensive to synthesize artificially
The main technical process for the separation and purification of swainsonine abroad is to first extract the plant sample or mycelium in an organic solvent, concentrate the extract, separate it through a cation exchange resin column and an agarose gel column, and finally recrystallize to obtain Swainsonine, its disadvantage is that the extraction process is complicated, the cost is high, and it cannot be mass-produced industrially

Method used

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Examples

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Embodiment 1

[0016] Take 1kg of Gansu thorn bean hay, heat-extract three times with 10 liters of 75% industrial ethanol for two hours each time, discard the material residue after filtration, and concentrate the extract under reduced pressure to obtain 180g of Gansu thorn bean total extract, with an extraction rate of 18%. . Add 200 mL of water to the extract to dissolve repeatedly, adjust the pH to 4 with concentrated hydrochloric acid, and remove fat-soluble impurities such as chlorophyll in the extract by countercurrent extraction with chloroform. The chloroform was discarded, the pH of the aqueous phase was adjusted to 9 with NaOH solution, and the total alkaloids were extracted by countercurrent extraction with n-butanol. The n-butanol was recovered under reduced pressure to obtain 11.5 g of crude total alkaloids with an alkali yield of 1.15%. Dissolve the crude alkaloid in methanol, mix samples with 100-200 mesh silica gel, dry column with 200-300 mesh silica gel, elute with chlorof...

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Abstract

The invention relates to a process for separating and extracting swainsonine from Oxytropis chinensis in Gansu. The present invention adopts the countercurrent extraction method to degrease and remove impurities with chloroform under acidic conditions, and then use n-butanol countercurrent extraction under alkaline conditions to obtain the crude total alkaloids. The obtained crude total alkaloids were separated by silica gel column chromatography to obtain crude swainsonine, and then recrystallized to obtain pure swainsonine. The purification process of the invention is completed in four steps, the extraction cost is low, the process is simple, the product purity is high, and the whole extraction process is suitable for industrialized production.

Description

technical field [0001] The invention relates to a natural product extraction and separation method, in particular to a process method for separating and extracting swainsonine from Gansu thorn bean. Background technique [0002] Oxy trop is kansuensis is a perennial herb of the genus Oxy trop is bean, mainly distributed in Qinghai, Gansu, Ningxia, Sichuan, Yunnan and other places in my country. The biological yield is huge. Every year, a large number of livestock die from poisoning in the densely growing pastoral areas. The losses to the local herdsmen are quite serious. It is one of the most serious poisonous weeds in the pastoral areas of northwest and southwest my country. The research on the toxic components of Gansu thorn bean shows that swainsonine is the main toxic component. Swainsonine is a large polar indolizidine alkaloid with a special inhibitory effect on α-mannosidase. In recent years, swainsonine has attracted the attention of researchers at home and abroad a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D463/00A61K31/437A61K36/48A61P35/00A61P37/02
Inventor 邸多隆黄新异
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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