Modified method for transforming gramineous crop by agrobacterium mediated flower-dipping method

A technology of gramineous crops and Agrobacterium, which is applied in the field of the improved Agrobacterium-mediated flower soaking method to transform gramineous crops, can solve problems such as unreported gramineous crops, and achieve the effect of great practical application value.

Active Publication Date: 2010-09-29
未名三农生物农业技术有限公司
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the flower soaking method has been applied to the Brassica crops of the dicotyledonous plants Brassicaceae and has been successfully transformed. There is no report on the grass crops transformed by the soaking flower method.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Modified method for transforming gramineous crop by agrobacterium mediated flower-dipping method
  • Modified method for transforming gramineous crop by agrobacterium mediated flower-dipping method
  • Modified method for transforming gramineous crop by agrobacterium mediated flower-dipping method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0021] Example, Transgenic plants of sweet sorghum, millet, and maize were obtained by the method of soaking flowers

[0022] 1. Flower soaking method to transform sweet sorghum, millet, and corn plants

[0023] 1. Experimental materials

[0024] Agrobacterium strain: The Agrobacterium strain is LBA4404. Contains plasmid vector pKAT1-AsRed.

[0025] Plasmid: pKAT1-AsRed, containing the red fluorescent protein reporter gene AsRed2 derived from jellyfish, with hpt selection marker gene, resistant to Hygmycin antibiotics. This vector was constructed by our laboratory. The target gene AsRed2 (purchased from Clontech Laboratories Inc, USA) was inserted into the expression vector pCAMBIA1301, and the seed-specific expression promoter GluA3 cloned from rice was inserted into the upstream of AsRed2 gene to obtain pKAT-AsRed Expression vector. Its map is like figure 1 As shown in the figure, T-DNA left border is the left border of T-DNA insertion; hpt is a plant selection marker gene, which...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides an improved process for transforming gramineous crops by floral dip method, including the following steps. Firstly, agrobacterium with target gene is suspended by infiltration culture medium which contains Silwet L-17, Triton X-100 and GA. Secondly, under the condition of vivo, the integral inflorescence or partial pistils of the plants which are ready to be transformed areimmerged in the infiltration culture medium, or the inflorescence or the pistils are sprayed with the infiltration culture medium, and the target gene is integrated with the genome of a target plant by the agrobacterium. By employing the genetically modified process, the invention resolves transformation problems of certain gramineous crops which are hard to be transformed by regular transformingmethod, thereby the invention has relatively high practical application value.

Description

Technical field [0001] The present invention relates to an improved method and application of Agrobacterium-mediated flower soaking method for transforming gramineous crops, in particular to a method and application of transforming sweet sorghum, millet and corn. Background technique [0002] Food production can be divided into two main types, one is to provide food for food, and the other is to provide raw material for bioenergy. Sweet sorghum is an important energy plant. The oil crisis in the 1970s triggered human research on biofuels instead of oil. The United States and Brazil succeeded in producing fuel ethanol from corn and sugarcane. Now, due to the gradual exhaustion of fossil energy and the need for environmental protection, the development of the biomass industry has become an important development strategy for many countries. my country's food is not so rich yet. There are other ways to produce liquid biofuels. Sweet sorghum has a high photosynthetic efficiency an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82C12N15/87C12N15/84
Inventor 夏勉张会新凌莉
Owner 未名三农生物农业技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap