Alstonia-leaf medicinal materials and medicament testing method for its preparation

A detection method and lampstand leaf technology are applied in the directions of drug combination, pharmaceutical formulation, material separation, etc., which can solve the problems of poor specificity of the determination method and inability to meet the technical requirements of traditional Chinese medicine.

Active Publication Date: 2008-09-24
YUNNAN PHYTOPHARML
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of drug quality analysis, the "Drug Standards of Yunnan Province" only uses a simple alkaloid precipitation reaction to identify the medicinal materials of Dengtai Leaf, and there is no content determination item; The titration method is used

Method used

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  • Alstonia-leaf medicinal materials and medicament testing method for its preparation
  • Alstonia-leaf medicinal materials and medicament testing method for its preparation
  • Alstonia-leaf medicinal materials and medicament testing method for its preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Analysis and detection of the leaves of the lampstand leaf medicinal material-1

[0050] 1. Isolation and structure identification of compounds 1 and 2

[0051] (1) Extraction and separation

[0052] After crushing 50kg of the medicinal material of the lampstand tree, it is extracted with 95% ethanol under reflux, and the solvent is recovered under reduced pressure to obtain an extract. The alcohol extraction extract is mixed with water, then extracted with ethyl acetate and n-butanol in sequence, and the n-butanol part is recovered to obtain a flavonoid glycoside mixture. The flavonoid glycoside mixture was decolorized by MCI column, and methanol was used as eluent to enrich flavonoid glycosides. The enriched flavonoid glycosides were repeatedly separated by Sephadex LH-20 chromatography, using methanol as the eluent, combined with conventional purification methods such as recrystallization, to obtain compounds 1 and 2.

[0053] (2) Structural identificat...

Embodiment 2

[0076] Example 2: Analysis and detection of the leaves of the lampstand leaf medicinal material-2

[0077] The method is the same as in Example 1, but the developing solvent of the TLC analysis is changed to: ethyl acetate-butanone-formic acid-water=5:5:1:1. Test result: consistent with embodiment 1 judgment.

Embodiment 3

[0078] Example 3: Analysis and detection of the leaves of the lampstand leaf medicinal material-3

[0079] The method is the same as in Example 1, but the developing solvent of the TLC analysis is changed to: ethyl acetate-butanone-formic acid-water=5:3:0.5:1. Test result: consistent with embodiment 1 judgment.

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Abstract

The invention relates to a medicinal material of alstonia-leaf and the drug detecting method of the pharmaceutical preparations of alstonia-leaf. The invention is characterized in that a thin-layer chromatography is adopted to detect the effective ingredients of kaempferol-3-O-Beta-D-galactose-(2-1)-O-Beta-D-xylopyranoside and quercetin-3-O-Beta-D-galactose-(2--1)-O-Beta-D- xylopyranoside of the alstonia-leaf and the pharmaceutical preparations of alstonia-leaf qualitatively. Spectrophotometry is adopted to realize a quality analysis and control of the alstonia-leaf and the pharmaceutical preparations of alstonia-leaf. The invention overcomes the deficiencies that the prior art can only detect the alkaloid components yet fail to detect a type of more important active ingredient flavonoid glycoside in the alstonia-leaf and the pharmaceutical preparations of alstonia-leaf . The method of the invention can precisely detect flavonoid glycoside active-ingredient compound 1, flavonoid glycoside active-ingredient compound 2 and the contents in the alstonia-leaf and the pharmaceutical preparations of alstonia-leaf. The method can be used for evaluating the quality of alstonia-leaf and detecting the quality analysis of the pharmaceutical preparations of various types adopting alstonia-leaf as the medicinal ingredient, such as granules, tablets, capsules, oral liquid and so on. The method is directly applicable to an actual production process and a quality analysis of the product.

Description

technical field [0001] The invention relates to the technical field of drug analysis and detection, in particular to a drug detection method for the medicinal material and preparations thereof. Background technique [0002] Alstonia Scholaris (L.) R.Bro. is a plant of Apocynaceae, which is mainly distributed in southern Yunnan and is rich in resources. Dengtai Leaf is a natural medicine used by many ethnic groups. It is used to treat respiratory diseases such as lung-heat cough, phlegm, and bronchitis. The medicinal materials have been recorded in "Drug Standards of Yunnan Province" and "Chinese Pharmacopoeia". Using Dengtai leaf as raw material, Dengtai leaf granules, tablets, capsules, oral liquid and other preparations have been developed. [0003] For the chemical research on the leaves of the lampstand, Dr. Zhu Weiming's thesis "Preliminary Study on the Chemistry of Five Kinds of Medicinal Plant Resources", Dr. Zhu Hua's thesis "Study on the Chemistry and Active Compon...

Claims

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Application Information

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IPC IPC(8): A61K36/24A61P11/00G01N21/25G01N30/90A61K127/00
Inventor 饶高雄惠婷婷郭文朱丽萍
Owner YUNNAN PHYTOPHARML
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