Qualitative analysis detection method for low polarity sugar-reducing chemical medicament in traditional Chinese medicine

Abstract

The invention discloses a qualitative analysis detection method for illegally mixed high-polar chemical anti-diabetic components in anti-diabetic traditional Chinese medicine products. The method comprises the following steps that: 1) high efficient liquid phase chromatography conditions: an ammonium acetate-triethylamine-acetonitrile moving phase system and a C18 chromatographic column with certain specification are used, the wavelength is detected by ultraviolet, and the flow rate is 1.0ml / min; 2) analysis result: glibenclamide, glipizide, gliclazide, glimepiride, gliquidone, repaglinide, nateglinide, rosiglitazone and pioglitazone hydrochloride can realize the complete separation; 3) result judgment: when retention time of a chromatographic peak in an anti-diabetic traditional Chinese medicine product is consistent with that of anti-diabetic medicine in the step 2) and the apparent absorption is shown out, which indicate that the anti-diabetic medicine is contained in the sample tobe tested. The method has the advantages of quickness, simplicity, convenience, high sensitivity, strong specialization, broad coverage and so on.

Description

technical field [0001] The invention provides a qualitative analysis and detection method for illegally mixing low-polarity hypoglycemic chemical drugs in hypoglycemic traditional Chinese medicine products, which belongs to the drug quality detection system. Background technique [0002] Diabetes has become a worldwide health problem, and the prevalence of diabetes in my country is also increasing year by year. The new patients are mainly type 2 diabetes, and oral hypoglycemic drugs play an important role in the treatment of such patients. In recent years, biguanides and α-glucosidase inhibitors are commonly used clinical hypoglycemic drugs. Some traditional Chinese medicine hypoglycemic preparations have also appeared in the folk. Folk Chinese medicine preparations are pure Chinese medicine preparations with few side effects and can be taken in large quantities. Clinically, patients who take these Chinese medicine hypoglycemic drugs are poisoned. Therefore, it is necessary...

AI Technical Summary

Problems solved by technology

However, this process requires gradient elution, which is slightly complicated, and the separation time is long, which is not conducive to rapid identification

[0011] However, the above method can only detect one or two types of hypoglycemic chemical drugs, and the types of drugs detected are uneven, and there is no systematic classification research based on the properties of the drugs themselves.

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