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Qualitative analysis detection method for low polarity sugar-reducing chemical medicament in traditional Chinese medicine

A detection method and qualitative analysis technology, applied in the direction of analyzing materials, testing pharmaceutical preparations, measuring devices, etc., can solve the problems of long separation time, complexity, unfavorable rapid identification, etc., and achieve the effect of strong specificity, wide coverage and high sensitivity

Inactive Publication Date: 2011-06-08
北京市东城区药品检验所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this process requires gradient elution, which is slightly complicated, and the separation time is long, which is not conducive to rapid identification
[0011] However, the above method can only detect one or two types of hypoglycemic chemical drugs, and the types of drugs detected are uneven, and there is no systematic classification research based on the properties of the drugs themselves.

Method used

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  • Qualitative analysis detection method for low polarity sugar-reducing chemical medicament in traditional Chinese medicine
  • Qualitative analysis detection method for low polarity sugar-reducing chemical medicament in traditional Chinese medicine
  • Qualitative analysis detection method for low polarity sugar-reducing chemical medicament in traditional Chinese medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] 1. High performance liquid chromatography conditions

[0053] The mobile phase is ammonium acetate-triethylamine-acetonitrile mobile phase system, composed of A and B; A: a mixed solution of 0.01mol / L ammonium acetate and 0.02mol / L triethylamine, and adjust the pH to 5.5 with acetic acid, B It is acetonitrile; the volume ratio of A:B is 61:39; the flow rate is 0.8-1.2ml / min; the flow rate of the mobile phase is 1.0ml / min. The chromatographic column adopts C18 chromatographic column, the column temperature is 30°C, the specification is 5 μm, 4.6mm×150mm. The ultraviolet detection wavelength is 210nm. The injection volume was 20 μl.

[0054] 2. Analysis of the reference solution.

[0055] Mix low-polarity hypoglycemic drugs together, glipizide, glibenclamide, rosiglitazone, glimepiride, nateglinide, gliclazide, pioglitazone hydrochloride at a concentration of 20 μg / ml, Swiss The concentration of Glinide and Gliquinone is 30 μg / ml, and the analysis is carried out under...

Embodiment 2

[0061] In the high performance liquid chromatography conditions, the flow rate of the mobile phase is 0.8ml / min, the column temperature is 20° C., and the rest are the same as in Example 1.

Embodiment 3

[0063] In the high performance liquid chromatography conditions, the flow rate of the mobile phase is 1.2ml / min, the column temperature is 45° C., and the rest are the same as in Example 1.

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Abstract

The invention discloses a qualitative analysis detection method for illegally mixed high-polar chemical anti-diabetic components in anti-diabetic traditional Chinese medicine products. The method comprises the following steps that: 1) high efficient liquid phase chromatography conditions: an ammonium acetate-triethylamine-acetonitrile moving phase system and a C18 chromatographic column with certain specification are used, the wavelength is detected by ultraviolet, and the flow rate is 1.0ml / min; 2) analysis result: glibenclamide, glipizide, gliclazide, glimepiride, gliquidone, repaglinide, nateglinide, rosiglitazone and pioglitazone hydrochloride can realize the complete separation; 3) result judgment: when retention time of a chromatographic peak in an anti-diabetic traditional Chinese medicine product is consistent with that of anti-diabetic medicine in the step 2) and the apparent absorption is shown out, which indicate that the anti-diabetic medicine is contained in the sample tobe tested. The method has the advantages of quickness, simplicity, convenience, high sensitivity, strong specialization, broad coverage and so on.

Description

technical field [0001] The invention provides a qualitative analysis and detection method for illegally mixing low-polarity hypoglycemic chemical drugs in hypoglycemic traditional Chinese medicine products, which belongs to the drug quality detection system. Background technique [0002] Diabetes has become a worldwide health problem, and the prevalence of diabetes in my country is also increasing year by year. The new patients are mainly type 2 diabetes, and oral hypoglycemic drugs play an important role in the treatment of such patients. In recent years, biguanides and α-glucosidase inhibitors are commonly used clinical hypoglycemic drugs. Some traditional Chinese medicine hypoglycemic preparations have also appeared in the folk. Folk Chinese medicine preparations are pure Chinese medicine preparations with few side effects and can be taken in large quantities. Clinically, patients who take these Chinese medicine hypoglycemic drugs are poisoned. Therefore, it is necessary...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N33/15
Inventor 杨秉呼赵秀梅朝克图刘亚琴侯扬尤立华叶萌聂然孙建慧马家华
Owner 北京市东城区药品检验所
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