Syringeability cardiac muscle tissue engineering products based on thermo-sensitive chitosan hydrogel

A tissue engineering and temperature-sensitive technology, applied in medical science, prosthesis, etc., to achieve the effects of improving retention rate and survival rate, mild implementation conditions, and increasing the wall thickness of the infarcted area

Inactive Publication Date: 2008-10-22
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

Studies have found that the complex of chitosan and sodium glycerophosphate has good fluidity at room temperature, gels and forms hydrogel at physiological body temperature (37°C), and is an excellent injecta

Method used

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  • Syringeability cardiac muscle tissue engineering products based on thermo-sensitive chitosan hydrogel
  • Syringeability cardiac muscle tissue engineering products based on thermo-sensitive chitosan hydrogel
  • Syringeability cardiac muscle tissue engineering products based on thermo-sensitive chitosan hydrogel

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Experimental program
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Embodiment Construction

[0021] Preparation of chitosan hydrogel: Place 200mg of chitosan in a 10ml small beaker, add 9ml of 0.1% acetic acid, after the chitosan is completely dissolved, autoclave sterilization (121℃, 20min), and place 5g of β-glycerophosphate sodium in 10ml small beaker, add 10ml culture medium and filter sterilization with 0.22μm filter membrane, after 250mg hydroxyethyl cellulose is irradiated and sterilized, dissolve into 2.5% solution with 10ml culture medium, the three can be mixed according to the ratio of 4:1:1 The liquid gel with neutral pH and good fluidity at room temperature.

[0022] Histocompatibility test of chitosan hydrogel: 0.1ml of chitosan hydrogel was injected and implanted into the thigh muscles of SD rats, and they were sacrificed at 2, 4, 6, and 8 weeks respectively. Two rats were taken each time and the specimens were taken out After that, paraffin sections were performed and HE staining was performed to observe the inflammatory reaction. 2 weeks after implantatio...

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Abstract

The invention discloses an injective myocardial tissue engineering product basing on temperature responsive chitosan hydrogel and more particularly relates to liquid temperature responsive chitosan hydrogel which is used as stent material that is combined with seed cells from different sources, such as embryonic stem cells, mesenchymal stem cells, human fetal cadiacmyocytes, etc., and is injected and transplanted into the specific region of an animal myocardial infarction model for observing the condition of repairing the myocardial infarction region. Constructed by the stent material, the injective myocardial tissue engineering product can improve the retention rate and the survival rate of the seed cells, can promote the regeneration of myocardial tissues, can increase the wall thickness of the infarction region and can remold the shape of an original ventricular and improve the heart function. The product is provided with the injective character and is convenient for the treatment operation, thereby avoiding risks brought by the operations of cardioplegia arrest, extracorporeal circulation, etc. The injective myocardial tissue engineering product basing on the temperature responsive chitosan hydrogel has the advantages of simple operation process and mild implementation condition, provides a new product for the myocardial tissue engineering and has great significance for the clinical development of the tissue engineering myocardial treatment on heart diseases.

Description

Technical field [0001] The invention belongs to the field of tissue engineering, and particularly relates to a myocardial tissue engineering product manufactured by using temperature-sensitive chitosan hydrogel as a scaffold. Background of the invention [0002] The morbidity and mortality of ischemic heart disease and subsequent heart failure are increasing year by year around the world. Cardiovascular diseases have become one of the main causes of death and disability among the population, especially the elderly, and are the main cause in the medical profession today. One of the difficult problems. Current treatment methods include drugs, coronary interventional therapy, coronary artery bypass surgery, etc. Although these methods can alleviate the symptoms of patients to a certain extent, it is difficult to fundamentally restore the number of myocardial cells and improve the function of diastolic and contraction of the heart. Heart disease patients often need organ transplantat...

Claims

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Application Information

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IPC IPC(8): A61L27/38
Inventor 王常勇卢文宁郝彤吕双红段翠密王滟濛李晶
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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