Syringeability cardiac muscle tissue engineering products based on thermo-sensitive chitosan hydrogel

Abstract

The invention discloses an injective myocardial tissue engineering product basing on temperature responsive chitosan hydrogel and more particularly relates to liquid temperature responsive chitosan hydrogel which is used as stent material that is combined with seed cells from different sources, such as embryonic stem cells, mesenchymal stem cells, human fetal cadiacmyocytes, etc., and is injected and transplanted into the specific region of an animal myocardial infarction model for observing the condition of repairing the myocardial infarction region. Constructed by the stent material, the injective myocardial tissue engineering product can improve the retention rate and the survival rate of the seed cells, can promote the regeneration of myocardial tissues, can increase the wall thickness of the infarction region and can remold the shape of an original ventricular and improve the heart function. The product is provided with the injective character and is convenient for the treatment operation, thereby avoiding risks brought by the operations of cardioplegia arrest, extracorporeal circulation, etc. The injective myocardial tissue engineering product basing on the temperature responsive chitosan hydrogel has the advantages of simple operation process and mild implementation condition, provides a new product for the myocardial tissue engineering and has great significance for the clinical development of the tissue engineering myocardial treatment on heart diseases.

Description

Technical field [0001] The invention belongs to the field of tissue engineering, and particularly relates to a myocardial tissue engineering product manufactured by using temperature-sensitive chitosan hydrogel as a scaffold. Background of the invention [0002] The morbidity and mortality of ischemic heart disease and subsequent heart failure are increasing year by year around the world. Cardiovascular diseases have become one of the main causes of death and disability among the population, especially the elderly, and are the main cause in the medical profession today. One of the difficult problems. Current treatment methods include drugs, coronary interventional therapy, coronary artery bypass surgery, etc. Although these methods can alleviate the symptoms of patients to a certain extent, it is difficult to fundamentally restore the number of myocardial cells and improve the function of diastolic and contraction of the heart. Heart disease patients often need organ transplantat...

AI Technical Summary

Problems solved by technology

Studies have found that the complex of chitosan and sodium glycerophosphate has good fluidity at room temperature, gels and forms hydrogel at physiological body temperature (37°C), and is an excellent injectable cell scaffold. Used in cartilage and bone tissue engineering, but using it as a scaffold for the research and development of injectable myocardial tissue engineering products has not been reported so far

Images