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Collagen protein and collagen polypeptides, preparation thereof and applications

A collagen polypeptide and collagen technology, which is applied in the fields of peptide/protein components, animal/human proteins, specific peptides, etc., to achieve the effects of considerable economic benefits, low cost, and simple process equipment

Active Publication Date: 2010-12-22
史宗洁
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is unpredictable for those skilled in the art whether glucosamine is used in combination with arthritis medicines to exert a synergistic effect.

Method used

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  • Collagen protein and collagen polypeptides, preparation thereof and applications
  • Collagen protein and collagen polypeptides, preparation thereof and applications
  • Collagen protein and collagen polypeptides, preparation thereof and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Preparation of Collagen and Polypeptide Using Tilapia Scale as Raw Material

[0045] Wash 100 grams of decalcified tilapia scales with water, add deionized water at 1000°C and heat up to 85°C for 30 minutes, then cool down to 45°C, adjust the pH value to 8.0, add 3 grams of alkaline protease, degrade for 2 hours, and then re-degrade under the same conditions Add 4 grams of neutral protease, react for 2 hours, the scales are all dissolved, then raise the temperature to 90°C, keep warm for 15 to 30 minutes to inactivate the enzyme, add an appropriate amount of flocculant diatomite and activated carbon (mixed at 1:1) to settle for 8 Take the supernatant directly; the supernatant is distilled under reduced pressure at a vacuum degree of 0.06-0.10Mpa and a temperature of 40-65°C, and finally spray-dried to obtain solid protein polypeptide powder.

[0046] After determination, among them: Hydroxyproline content: 9.4%; Hydroxylysine, 1.4%.

Embodiment 2

[0047] Example 2 Preparation of Collagen and Polypeptide Using Tilapia Skin as Raw Material

[0048] Weigh 100g of dried fish skin into a 1000ml beaker, wash at room temperature until the pH value is about 7, heat to 80°C for 30 minutes, cool to 50°C, adjust the pH value to 8, add 2.5 grams of alkaline protease, and react for 3 hours ; Adjust the temperature to 45°C, add 4 grams of neutral protease, and stir at a constant temperature for 3 hours; heat to 85°C for 15 minutes to inactivate the enzyme. Add an appropriate amount of flocculant diatomaceous earth and activated carbon (mixed at 1:2) to settle for 8 hours, and take the supernatant directly. The supernatant is distilled under reduced pressure at a vacuum degree of 0.06-0.10Mpa and a temperature of 40-65°C, and finally spray-dried to obtain solid protein polypeptide powder.

[0049] Product testing results: Hydroxyproline content: 9.38%; Hydroxylysine, 1.31%.

Embodiment 3

[0050] Embodiment 3 prepares collagen and polypeptide with cod skin as raw material

[0051] Weigh 100g of dried cod skin into a 1000ml beaker, wash at room temperature until the pH value is about 7, heat to 80°C for 30 minutes, cool to 50°C, adjust the pH value to 8, add 4 grams of trypsin respectively, and react 6 Hours; adjust the temperature to 45°C, add 3 grams of 1398 protease, and stir at a constant temperature for 5 hours; heat to 85°C for 15 minutes to inactivate the enzyme.

[0052] After flocculation and precipitation filtration, the filtrate is distilled under reduced pressure at about 50°C, and finally spray-dried to obtain fish skin collagen polypeptide powder.

[0053] Product testing results: Hydroxyproline content: 7.3%; Hydroxylysine, 1.3%

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Abstract

The invention provides a collagen and a collagen polypeptide, wherein, the weight percentage of hydroxylysine is more than 1.3 percent. The invention also provides the preparation technology and the application of the collagen and the collagen polypeptide. The invention can make full use of inexpensive raw materials including the fish skin and fish scale of tilapias to produce products with high added value. The collagen polypeptide is used for treating arthritis with a definite drug effect, and the clinical dose is considerably less than other collagen polypeptides. The collagen polypeptide can solve the pollution problem of fish skin wastes, and at the same time avoid the risk of infectious diseases which can be caused by the collagen of land animals. The collagen and the collagen polypeptide have the advantages of considerable economic benefits, energy conservation and environmental protection, simple process equipment, low cost and easy industrialization.

Description

technical field [0001] The invention relates to a collagen protein and a collagen polypeptide, in particular to a collagen protein and a collagen polypeptide derived from fish skin or fish scales, and an enzymatic hydrolysis preparation method and application thereof. Background technique [0002] At present, collagen and collagen peptides have been widely used in medicine, chemical industry, food and other fields, including medical materials, medicines, health care products, and beauty and skin care products, especially the unique hemostatic function of collagen, the promotion of cell growth and wound healing. As well as biocompatibility and biodegradability, the advantages of collagen as a raw material for health products are very obvious. This is also a research hotspot at home and abroad in recent years. Therefore, making full use of collagen biomass resources has broad application prospects. [0003] At present, the raw materials for the preparation of collagen mainly...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/78C12P21/06A61K8/65A61K38/39A61K31/7008A61P19/02
Inventor 史宗洁胡琦敏唐少锋
Owner 史宗洁
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