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Applications of gastrodine in preparing cysteine proteinase-3 active inhibitor

A technology of cysteine ​​protease and activity inhibitor, applied in the field of pharmacy

Inactive Publication Date: 2008-12-17
XUANWU HOSPITAL OF CAPITAL UNIV OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the effects of gastrodin and its congeners on the activity of Caspase-3 have not been reported

Method used

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  • Applications of gastrodine in preparing cysteine proteinase-3 active inhibitor
  • Applications of gastrodine in preparing cysteine proteinase-3 active inhibitor
  • Applications of gastrodine in preparing cysteine proteinase-3 active inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: the influence of gastrodin on the activity of Caspase-3

[0019] First, the purpose

[0020] Using Western blot immunoblotting and cell immunofluorescence staining experimental techniques and methods to observe the effect of effective natural drug monomers on the expression of apoptosis execution gene caspase3 protein, and then explore the anti-apoptosis of natural drug monomers from the aspect of apoptosis gene expression mechanism of action.

[0021] 2. Materials and methods

[0022] 1. Experimental materials and equipment

[0023] (1) Cell source

[0024] Mutant PS1M146L and wild-type APP751 double transfection cell line (PS1M146L / APP 751 ), wild-type PS1 and wild-type APP751 double-transfected Chinese hamster ovary tumor cell line (TW), and Chinese hamster ovary tumor cell line (CHO) were constructed by the Center for Neurological Diseases, Harvard Medical School, and approved by the Institute of Basic Research, Chinese Academy of Medical Sciences....

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PUM

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Abstract

The invention discloses the application of gastrodine to the preparation of caspase-3 active inhibitor and belongs to the field of pharmacy. The gastrodine is applied to the preparation of caspase-3 active inhibitor, the caspase-3 active inhibitor can be used for treating diseases including neurodegenerative diseases, cardiovascular and cerebrovascular diseases, osteoarthritis, rheumatoid arthritis, and the like, specifically such as Parkinson disease, Alzheimer disease, amyotrophic lateral sclerosis, Huntington disease, traumatic brain injury, spinal cord injury, severe hepatitis, liver dysfunction, osteoarthritis, rheumatoid arthritis, acquired immunodeficiency syndrome, myocardial infarction, cerebral ischemia and the like. The application of gastrodine has the advantage of blocking the specific protease needed by apoptosis without any adverse reaction which is caused by the application of the majority of broad spectrum drugs.

Description

technical field [0001] The invention relates to the application of gastrodin in the preparation of cysteine ​​protease-3 (Caspase-3) activity inhibitor, which belongs to the field of pharmacy. Background technique [0002] The occurrence of apoptosis is a complex protease cascade reaction process guided by the Caspase family. Although the Caspases involved in the apoptosis process induced by different cells or different signal transduction pathways are different, Caspase-3 is the key to apoptosis. It is the only way of protease cascade reaction, and it is also the key enzyme and executor of apoptosis. Gene knockout experiments and animal models have shown that blocking caspase activity blocks apoptosis in vitro and in vivo. Therefore, Caspase-3 is expected to become a new target for the treatment of neurodegenerative diseases caused by abnormal apoptosis, cardiovascular and cerebrovascular diseases, rheumatoid arthritis, etc., which requires the development of effective Cas...

Claims

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Application Information

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IPC IPC(8): A61K31/7034A61P25/28A61P9/00A61P1/16A61P29/00A61P31/18
Inventor 魏翠柏贾建平梁平
Owner XUANWU HOSPITAL OF CAPITAL UNIV OF MEDICAL SCI
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