Simultaneous quantitative determination process for various enteric pathogenic bacteria in environment water body example

Abstract

The invention discloses a method for rapidly, quantitatively and simultaneously detecting a plurality of intestinal tract pathogenetic bacteria in an environmental water sample, which applies an intestinal tract pathogenetic bacteria PCR universal primer to build a real-time fluorescence quantitative polymerase chain reaction system for quantitatively measuring bacterial cell densities of Escherichia coli, comma bacilli, dysentery bacilli and salmonella typhi in the environmental water, and the method can shorten the time of water sample analysis from sampling to report completing to a plurality of hours, the measuring results are stable, and the repeatability is good. The standard deviation of the measuring results of the invention are all lower than the MF test results, the test resultsare stable, and the detecting repeatability is greatly improved.

Description

technical field [0001] The present invention relates to the detection method of environmental water body, particularly a kind of simultaneous quantitative detection method of multiple intestinal pathogenic bacteria in environmental water body sample, this method uses real-time fluorescence quantitative polymerase chain reaction (Real-time Quantitative Polymerase Chain Reaction, QPCR) The method detects enteric pathogenic bacteria in water, and is suitable for the quantitative detection of Escherichia coli, Vibrio cholerae, Shigella dysenteriae and Salmonella typhrmurium in water. Background technique [0002] The aggravation of urban pollution has caused more untreated sewage to be discharged directly to the surface environment, causing many diseases and even death to humans, especially in developing countries. In particular, diseases transmitted by water, such as dysentery, typhoid, cholera, and diarrhea common in travel, are all caused by pathogenic bacteria. Therefore, r...

AI Technical Summary

Problems solved by technology

First, the amount of pathogenic bacteria in the water body is usually very small, and the sampling and counting process may lead to large errors

Secondly, the bacterial culture method is usually time-consuming and laborious, and the detection is single

Third, some pathogenic microorganisms in the environment are difficult or even impossible to cultivate, but they can still cause disease

Unfortunately, the sensitivity of these methods is unstable and affected by many factors

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