Cultivation method for vegetative organ exsomatized treeing sprout of bearing tree of mandarin orange

A technology of vegetative organs and cultivation methods, which is applied in the field of in vitro cluster bud cultivation of tangerine adult tree vegetative organs, and achieves the effect of extensive practical value

Inactive Publication Date: 2009-01-14
JIANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In my country, the research work on the rapid propagation of citrus by tissue culture is relatively extensive. Since the report of Wang Dayuan in the 1970s, there have been many documents reporting the production of in vitro adventitious buds or cluster buds of various plants of the family Citrus. Most of the explants are tissues that have not passed through childhood, and there are few reports of regenerated shoots from adult vegetative tissue culture

Method used

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  • Cultivation method for vegetative organ exsomatized treeing sprout of bearing tree of mandarin orange
  • Cultivation method for vegetative organ exsomatized treeing sprout of bearing tree of mandarin orange

Examples

Experimental program
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Effect test

Embodiment 1

[0024] (1), select strong and healthy Nanfeng tangerine adult excellent plant fruit tree without damage by disease and insect, get the adult state stem that pumps out within 20d (day) in the same year and pump out new shoots,

[0025] (2) Wash away the dust with tap water, and then dissolve an appropriate amount of acidic commercial toothpaste in water, soak the tender branches for 6-10 minutes, then brush the branches and leaves with a soft brush, rinse with tap water; then soak with alkaline detergent for 6 ~10min, rinse in tap water, rinse with distilled water for 1 to 3 times after trimming the old leaves and branches, absorb the water droplets with filter paper, put them into a sterile container for surface sterilization; % ethanol solution for 20-30s, washed with sterile water for 2-4 times, and then 0.1%-0.5% HgCl 2 Soak new branches of citrus for 6-10 minutes, pour off the medicinal solution, and rinse with sterile water for 5-6 times. Then under aseptic conditions, c...

Embodiment 2

[0035] Select healthy adult tangerine fruit trees with no pests and diseases, and take new shoots from adult stems within 20 days (days) of the current year. When cleaning and disinfecting the explants, first soak them in 75% ethanol solution for 20-30 seconds, and rinse them with sterile water. 2 to 4 times, and then soak the new citrus shoots in 0.1% for 10 minutes. Under sterile conditions, cut the new citrus shoots into small sections (with stem nodes) with a length of about 0.5 to 1 cm, and inoculate them on the prepared medium for cultivation. (1) Axillary bud initiation medium: MS+6-BA0.1mg / L (the same unit below)+Kt0.1+NAA0.01; (2) Bud proliferation medium: improved MS+6-BA0.2+Kt0. 04+Zt0.2+NAA0.01, others are the same as in Example 1.

Embodiment 3

[0037] (1) Axillary bud initiation medium: improved MS+6-BA0.2mg / L (the unit is the same below)+Kt0.6+NAA0.1; (2) Bud proliferation medium: improved MS+6-BA0.3+Kt0 .4+Zt0.5+NAA0.1, others are identical with embodiment 1 or 2.

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Abstract

The invention discloses a method which comprises the following steps: strong mature tangerine plants which have no disease and insect damage and have fruit are selected; young branch within 20 days and randomly selected to grow in the year are taken as explants; the explants are cleaned and disinfected, and then inoculated and cultured. Axillary bud primary culture medium: MS or improved MS+6-BA0.1 to 0.6mg/l+Kt0.1 to 0.6mg/l+NAA0.01 to 0.1mg/l. Improved MS+6-BA0.2 to 0.5mg/l+Kt0.04 to 0.4mg/l+Zt0.2 to 0.5mg/l+NAA0.01 to 0.05mg/l. The culture method and the substrate prescription of the invention provide required environment conditions for the mature tangerine stem axillary bud vitro regeneration. Plenty of regeneration buds can be obtained if the method of the invention is used for the vitro regeneration of Nanfeng tangerine. The tangerine vitro regeneration buds can be used for germplasm preservation and batch high-grade germchit grafting and culture in test tubes.

Description

technical field [0001] The invention relates to a plant biotechnology, in particular to a method for cultivating clustered buds of tangerine adult tree vegetative organs in vitro. Background technique [0002] Citrus ranks first in the production of 100 fruits in the world. Its fruit is rich in nutrition, excellent in color, fragrance and taste. It can be eaten fresh or processed into fruit juice products. Nanfeng tangerine is a well-known fine variety of citrus tangerine. Nanfeng tangerine is derived from milk orange, and it is also a high-quality milk orange. The most prominent features are thin golden skin, sweet and sour taste, rich aroma, juicy and few cores. It is known as the "king of oranges" and is well-known at home and abroad. Unique fine citrus cultivars. [0003] The in vitro axillary bud cluster culture of adult stem organs of tangerine plays an important role in rapid propagation of high-quality seedlings and in vitro preservation of germplasm, and it can be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 涂艺声徐志祥蔡险峰张杨军
Owner JIANGXI NORMAL UNIV
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