Cultivation method for vegetative organ exsomatized treeing sprout of bearing tree of mandarin orange

A technology of vegetative organs and cultivation methods, which is applied in the field of in vitro cluster bud cultivation of tangerine adult tree vegetative organs, and achieves the effect of extensive practical value
CN101341854AInactive Publication Date: 2009-01-14JIANGXI NORMAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGXI NORMAL UNIV
Publication Date
2009-01-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method which comprises the following steps: strong mature tangerine plants which have no disease and insect damage and have fruit are selected; young branch within 20 days and randomly selected to grow in the year are taken as explants; the explants are cleaned and disinfected, and then inoculated and cultured. Axillary bud primary culture medium: MS or improved MS+6-BA0.1 to 0.6mg / l+Kt0.1 to 0.6mg / l+NAA0.01 to 0.1mg / l. Improved MS+6-BA0.2 to 0.5mg / l+Kt0.04 to 0.4mg / l+Zt0.2 to 0.5mg / l+NAA0.01 to 0.05mg / l. The culture method and the substrate prescription of the invention provide required environment conditions for the mature tangerine stem axillary bud vitro regeneration. Plenty of regeneration buds can be obtained if the method of the invention is used for the vitro regeneration of Nanfeng tangerine. The tangerine vitro regeneration buds can be used for germplasm preservation and batch high-grade germchit grafting and culture in test tubes.
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Description

technical field

[0001] The invention relates to a plant biotechnology, in particular to a method for cultivating clustered buds of tangerine adult tree vegetative organs in vitro. Background technique

[0002] Citrus ranks first in the production of 100 fruits in the world. Its fruit is rich in nutrition, excellent in color, fragrance and taste. It can be eaten fresh or processed into fruit juice products. Nanfeng tangerine is a well-known fine variety of citrus tangerine. Nanfeng tangerine is derived from milk orange, and it is also a high-quality milk orange. The most prominent features are thin golden skin, sweet and sour taste, rich aroma, juicy and few cores. It is known as the "king of oranges" and is well-known at home and abroad. Unique fine citrus cultivars.

[0003] The in vitro axillary bud cluster culture of adult stem organs of tangerine plays an important role in rapid propagation of high-quality seedlings and in vitro preservation of germplasm, and it can be...

Claims

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