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Anti-tumor Chinese medicine extract, and preparation method and uses thereof

An extract and anti-tumor technology, applied in anti-tumor drugs, drug combinations, pharmaceutical formulations, etc., can solve the problem of little inhibitory effect

Inactive Publication Date: 2009-01-28
ZHEJIANG AINUO BIOLOGICAL PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no mature method for extracting Clematis paniculata for antitumor drugs
Moreover, the current antitumor drugs have little inhibitory effect on the growth of leukemia cell line Molt-4, leukemia cell line HL60, brain cancer cell line U251, esophageal cancer cell line Eca-109 cell line, and liver cancer cell line BEL-7402 in vitro

Method used

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  • Anti-tumor Chinese medicine extract, and preparation method and uses thereof
  • Anti-tumor Chinese medicine extract, and preparation method and uses thereof
  • Anti-tumor Chinese medicine extract, and preparation method and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Clematis paniculata crude powder 5kg, add 8 times the amount of water, heat and reflux extraction twice, each time for 1h, filter, combine the extracts, concentrate to 1.0g crude drug / ml; centrifuge (4000 rpm) and pass through AB- Type 8 macroporous resin, the ratio of liquid medicine to dry resin is 1:5 based on the total flavonoid content of Clematis paniculata, the flow rate of the upper column is 2BV / h, and the diameter-to-height ratio of the resin is 1:5; the resin is washed with water for 3BV, and eluted The flow rate is 4BV / h, and the water eluent is discarded; 3BV is eluted with ethanol containing 70% water, and the flow rate is 2BV / h, and the ethanol eluate is collected; ), dried under reduced pressure to obtain Clematis paniculata extract. It is measured that the total flavonoid content of the extract is 27% (UV method; weight percentage), and the content of rutin is 0.45% (HPLC method, weight percentage).

Embodiment 2

[0023] Add 10 times the amount of 30% ethanol to 5kg of decoction pieces of Clematis paniculata, heat and reflux for extraction twice, each time for 1.5h, filter, combine the extracts, concentrate to 0.5g crude drug / ml; pass through D201 macroporous resin after filtration, The ratio of medicinal solution to dry resin based on the total flavonoid content is 1:9, the column flow rate is 3BV / h, and the diameter-to-height ratio of the resin is 1:7; the resin is washed with water for 5BV, the elution flow rate is 4BV / h, and the water eluent Discard; elute 10BV with ethanol containing 30% water, the flow rate is 4BV / h, collect the ethanol eluent; the ethanol solution is concentrated under reduced pressure to a relative density of 1.25 (50 ℃ thermal measurement), spray-dried to obtain Clematis paniculata Extract. It is measured that the total flavonoid content of the extract is 35% (UV method, weight percentage), and the rutin content is 1.1% (HPLC method, weight percentage).

Embodiment 3

[0025] Add 15 times the amount of 70% ethanol to 5 kg of clematis coniferous medicinal material, heat and reflux for extraction twice, each time for 1 hour, filter, combine the extracts, concentrate to 0.25 g crude drug / ml; pass through HPD101 after centrifugation (8000 rpm) type macroporous resin, the ratio of liquid medicine to dry resin is 1:6 based on the total flavonoid content, the column flow rate is 4BV / h, and the diameter-to-height ratio of the resin is 1:9; the resin is washed with water 2BV, and the elution flow rate is 3BV / h , the water eluent was discarded; 5BV was eluted with ethanol containing 70% water, and the flow rate was 3BV / h, and the ethanol eluate was collected; the ethanol solution was concentrated under reduced pressure to a relative density of 1.2 (50 ° C thermal measurement), spray-dried, Clematis paniculata extract. The measured total flavonoid content of Clematis paniculata is 70% (UV method, calculated as Clematis paniculata saponin D, weight perc...

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Abstract

The invention discloses an anti-tumor Chinese medicine extract, a preparation method and an application thereof; pulverized clematis terniflora is used or a clematis terniflora decoction piece is directly adopted, added with water or ethanol, heated, treated with reflux extraction for 2-5 times and then filtered; extract is merged, filtered or centrifugated, and then passes through macroporous resin; then water is firstly used for washing the resin, the water eluent is removed, then hydrous ethanol with the volume percentage of 30-95% is used for eluting, and the ethanol eluent is collected and concentrated into thick paste under reduced pressure, the thick paste undergoes decompressed concentration or spray drying so as to obtain the clematis terniflora extract which contains flavone and rutin no less than 20% and 0.1% respectively. The extract of the invention shows good in vitro inhibiting effect on the proliferation of leukemia Molt-4 cell strain, leukemia cell HL60, cerebral cancer U251 cell strain, esophagus cancer Eca-109 cell strain as well as liver cancer BEL-7402 cell strain. The anti-tumor Chinese medicine extract can be used for preparing the medicines for treating tumor, especially digestive system malignant tumor and the like diseases.

Description

【Technical field】 [0001] The invention relates to a novel antitumor traditional Chinese medicine extract, a preparation method thereof, and an application thereof in antitumor medicines. 【Background technique】 [0002] Tumor has become a common and frequently-occurring disease that seriously threatens human health. The number of deaths due to tumors in the world has reached 7 million people every year; while there are 2 million new cancer patients every year in China. The second leading cause of death in the country. [0003] At present, chemotherapy is one of the important means of clinical tumor treatment. Since nitrogen mustard was used to treat malignant lymphoma in the 1940s, great progress has been made in drug therapy in the past few decades, and the single drug therapy has entered the stage of combined chemotherapy and comprehensive chemotherapy. However, the existing antitumor drugs still have the disadvantages of excessive toxicity and lack of selectivity, and th...

Claims

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Application Information

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IPC IPC(8): A61K36/716A61P35/00
Inventor 吴淑英田景奎张琳彭晓国
Owner ZHEJIANG AINUO BIOLOGICAL PHARMA CO LTD
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