Medium pollen, preparation method and use thereof

Abstract

The invention discloses a medium pollen, a preparation method and the application thereof. The preparation method is as follows: tobacco male parent anther is collected and aired to cause the anther to loose power, a 50 mesh sieve is used for sieving out pollen and pollen sac, the pollen sac is collected, dried at 40-50 DEG C for 20-24 hours, and ground into powder; the pollen sac powder and the pure tobacco pollen are mixed evenly according to 1:1-4 of volume ratio, and the required medium pollen is obtained. The medium pollen is dipped by a cotton swab or a brush pen and applied to the female parent tobacco flower column head for artificial pollination. The use of the medium pollen improves effective utilization ratio of the tobacco pollen, decreases application amount of the pure pollen, saves manpower and material resources for collecting pollen, decreases planting area of the tobacco male parents, and lowers production cost of seed production by hybridization. The popularization and application of the preparation method thereof have good economic benefit and social benefit.

Description

Technical field The invention belongs to the technical field of artificial pollination, and relates to artificial assisted pollination of tobacco hybrid seed production, in particular to a medium pollen prepared by using tobacco pollen sacs. At the same time, the invention also relates to the preparation method and application of the medium pollen. Background technique In the hybrid seed production of tobacco sterile lines, artificial pollination is an important and essential task. Tobacco male parents have a small amount of pollen and are difficult to collect. Pollen collection in production is an extremely costly task. At present, in the process of hybrid seed production of tobacco sterile lines, pure tobacco pollen is used for artificial pollination, but this method uses a large amount of pollen, the effective utilization rate of pollen is low, and the cost is too high. my country’s tobacco sterile line varieties have a large planting area and require a lot of seeds. There...

AI Technical Summary

Problems solved by technology

In fact, tobacco anthers and pollen have homology, if anthers are used as pollen extender to prepare medium pollen, it will not have negative impact on pollen viability, and should be able to achieve better results, but this has not yet been seen in the prior art. There are relevant reports