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Base sequence detection apparatus and base sequence automatic analyzing apparatus

A base sequence and analysis device technology, which is applied in the field of base sequence automatic analysis devices, and can solve problems such as lack of data reproducibility, deviations in detection results, and fluctuations in measurement results

Inactive Publication Date: 2009-02-04
KK TOSHIBA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the case of using the above-mentioned device for measurement, when each process is completed, the operator needs to manually transfer the sample to the device for the next process, so time is limited.
In addition, since the operator is involved in the transfer between processes, the reproducibility of data between samples is lacking.
[0005] On the other hand, there is a problem that the reaction conditions in the reaction cell used for the reaction fluctuate the measurement results
In the case of measuring with a 3-electrode system with multiple working electrodes, the reaction environment of each working electrode varies, and the detection results also vary.

Method used

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  • Base sequence detection apparatus and base sequence automatic analyzing apparatus
  • Base sequence detection apparatus and base sequence automatic analyzing apparatus
  • Base sequence detection apparatus and base sequence automatic analyzing apparatus

Examples

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Embodiment 1

[0439] Next, an example of SNPs detection using the base sequence detection apparatus of the first embodiment described above will be described. Here, the base sequence of the SNPs applicable to the MxA-88 gene is G / G type or T / T type, or when G / T is different.

[0440] A DNA probe with a complementary sequence to the MxA gene is fixed in advance on the working electrode 501 of the base sequence detection sheet. Four types of probe DNA fragments in which bases at SNP positions were substituted with ATGC and a DNA fragment with a completely different sequence (referred to as a negative control) were immobilized on another electrode (working electrode 501 ). Here, each probe modified with cysteine ​​was placed at the N-terminus every 200 nL and left to stand for 1 hour to perform immobilization on the working electrode 501 made of Au. In this way, the printed circuit board 22 sealed with the prepared base sequence detection sheet 21 is attached to the sheet cassette 11 .

[04...

Embodiment 2

[0447] Next, an example of SNPs detection using the base sequence detection apparatus of the second embodiment described above will be described. Here, the base sequence of the SNPs applicable to the MxA-88 gene is G / G type or T / T type, or when G / T is different.

[0448] The working electrode on the substrate 714 is pre-immobilized with a DNA probe having a complementary sequence to the MxA gene. Four types of probe DNA fragments in which bases at SNP positions have been substituted with ATGC and DNA fragments with completely different sequences (referred to as negative controls) were respectively immobilized on other electrodes. Here, each probe modified with cysteine ​​was placed at the N-terminus every 200 nL and left to stand for 1 hour to perform immobilization on the working electrode 501 made of Au. In this way, the prepared substrate 714 is attached to the detection cassette 703 .

[0449] Then, the target DNA whose base at the SNP position is G type is dissolved in ...

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Abstract

Provided is a base sequence detection apparatus and a base sequence automatic analyzing apparatus. The base sequence detection apparatus is provided with a channel formed on a base sequence detection chip. Working electrodes are formed along the channel and include a probe immobilized thereon, counter electrodes are formed on the inner surface of the channel and, reference electrodes are formed on the inner surface of the channel. An introduction port introduces solution or air from the upstream side of the channel, a delivery port delivers the solution or air in the channel, and the sample is injected into the channel through a sample injection port.

Description

[0001] This application is a divisional application of the patent application with the application number 03801658.3, the filing date is July 30, 2003, and the invention title is "base sequence detection device and base sequence automatic analysis device". technical field [0002] The present invention relates to a base sequence detection device for detecting a base sequence and an automatic base sequence analysis device for automatically controlling the base sequence detection device and automatically analyzing measurement signals. Background technique [0003] In the past, for example, there were devices that only performed hybridization, devices that only performed electrochemical measurements after adding an intercalant after hybridization, or devices that automatically performed hybridization to washing with a buffer (for example, Patent Document 1: Japanese Special Table No. 9-504910). [0004] When the measurement is performed using the above-mentioned device, when ea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/403G01N27/416G01N33/543B01L3/00B01L9/00
Inventor 本乡祯人石川实石村多喜二若山茂弥永真司大木健司冈田纯
Owner KK TOSHIBA