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Active material extracted from Acutangular Anisodus root and uses thereof

A technology of active substances and samples, applied in the direction of application, biocide, animal repellent, etc., to achieve the effect of simple preparation

Inactive Publication Date: 2009-03-18
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far there is no report that the plant has activity against nematodes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Three thirds of the samples were dried by the drying method described in the summary of the invention above. Put 10g of 0.5cm long 3 / 3 dried sample into a 150ml Erlenmeyer flask, soak it in 100% acetone, let it stand at room temperature for 72 hours, filter the soaking solution, and concentrate it at a temperature not higher than 50°C to Balance, weigh, dissolve the sample with acetone, quantify it to 0.1g / ml, do the poisonous activity of the extract with the concentration of 5mg / ml and the concentration of 10mg / ml according to the above-mentioned experimental method, and use the corresponding concentration The acetone and the nematode suspension without three-thirds of the extract were used as controls, and the results are shown in Table 1.

[0022] Table 1 Lethality of acetone extract to pine xylophilus (%)

[0023]

[0024] The result shows: three-thirds active extract of the present invention has higher poisonous effect to pine wood nematode, and its acet...

Embodiment 2

[0026] Basically the same as Example 1, the difference is that the organic solvent used for extraction is 100% methanol. Put 10g of three-thirds dried sample into a 150ml conical flask, soak in 100ml of methanol, let it stand at room temperature for 72h, filter and concentrate the methanol extract obtained after quantitative (0.1g / ml) treatment as the test sample. Do 5mg / ml concentration and 10mg / ml concentration extract according to above-mentioned experimental method to the poisonous activity of pine xylophilus, and with the nematode suspension that contains methanol of corresponding concentration and does not add three-thirds extract as contrast, activity The results are shown in Table 2.

[0027] Table 2 The lethality of methanol extract to pine xylophilus (%)

[0028]

[0029] The result shows: three-thirds active extract of the present invention has higher poisonous action to pine wood nematode, and its methanol extract has stronger inhibitory action to nemato...

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PUM

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Abstract

The invention relates to an active matter which is extracted from acutangular anisodus root and the application thereof, which belongs to the technical field of biological pesticide. The active matter is obtained by the following steps: a. the collected entire plant of acutangular anisodus root is washed by water, and then the washed acutangular anisodus root is put into an oven and baked for 10 hours at the temperature of 50 DEG C; b. the baked acutangular anisodus root is sheared into small sections with the length of 0.5cm; c.10g of the sheared acutangular anisodus root is weighed is taken and placed into 150ml of triangular flasks respectively; wherein, one flask is added with 100ml of 100 percent acetone for extracting, and the other flask is added with 100ml of 100 percent methanol for extracting; the samples extracted by the two solvents are placed for 72 hours at the room temperature; d. the leach liquor of the samples processed under different conditions is respectively filtered, and concentrated at the temperature of 50 DEG C so as to obtain active extract of the acutangular anisodus root; and the extract of acetone is dissolved by the acetone, the extract of methanol uses the methanol to dissolve the sample, the quantity is fixed to be 0.1g / ml and the obtained sample is put into the refrigerator for spare. The active extract of the invention has the advantages of simple and convenient preparation, high efficiency and low toxicity and the like, and can be applied to preparing biological pesticide used for killing nematodes.

Description

Technical field: [0001] The invention relates to an active substance extracted from Sanfensan and its application, and belongs to the technical field of biological pesticides. Background technique: [0002] The pine wood nematode (Bursaphelenchus xylophilus) belongs to the class Nematoda, the order Tylenchida, the family Aphelenchoididae, and the genus Bursaphelenchus. Pine xylophilus disease caused by pine wood nematode is one of the most dangerous forest diseases in the world, and it is a devastating epidemic disease of pine trees. The disease has a short onset period, fast death of diseased trees, easy transmission, and difficult control. Therefore, research and development of high-efficiency and low-toxicity killing pine wood nematode (B.xylophilus) has received widespread attention. Plants are one of the main sources of active natural products, and finding natural metabolites from plants that are toxic to B. xylophilus has become one of the focuses of nematode biologi...

Claims

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Application Information

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IPC IPC(8): A01N65/00A01P7/00
Inventor 李国红张克勤
Owner YUNNAN UNIV
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