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Lonicera confusa extract and preparation method and application thereof

A silver flower extract and extract technology are applied in the directions of drug combination, pharmaceutical formula, plant raw material, etc., can solve problems such as being unsuitable for industrialized production, unfavorable to pharmaceutical and therapeutic fields, complex methods, etc., and are suitable for industrialized production, The drug has good anti-cancer effect and the effect of wide source of raw materials

Inactive Publication Date: 2009-04-15
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, a variety of saponins in Jasmine Japonica have been isolated and identified, but most of these separation and extraction methods belong to laboratory research methods. The method is complicated, the cost is high, and the yield of the isolated saponins is low. It is not suitable for industrial production, and the content of ingredients in the extract is not clear, which is not conducive to the application in pharmaceutical and therapeutic fields
[0003] Our laboratory has been engaged in the separation, extraction and research of the medicinal components of Limonosa japonica for many years. In our previous application 200610039294.4, we disclosed the total saponin extract extracted from Lonicerae argentina. The total saponin content in this extract is 50% -90%, wherein Lonicera cinerea Lonicera saponin B: Lonicera cinerea Lonicera saponin A=12~6:1, also discloses its preparation method and the purposes for preventing and treating liver cancer at the same time, in our another The application 200710023243.7 discloses the preparation method of the monomeric component Lonicera tomentosa saponin B and its application in the treatment of liver cancer, breast cancer and cervical cancer. However, the yield of the extraction method in the above application is still relatively low. The disclosed treatment limited types of cancer

Method used

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  • Lonicera confusa extract and preparation method and application thereof
  • Lonicera confusa extract and preparation method and application thereof
  • Lonicera confusa extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027]The flowers and buds of Lonicerae pilosula are extracted with 8 times the volume of 60% ethanol at 90 degrees Celsius, and the solvent is recovered to one-fifth of the original volume of the extract, cooled to 0 degrees Celsius, filtered to obtain the filtrate, and the filtrate is added 8% KOH was hydrolyzed at 80 degrees Celsius for 10 hours, extracted with n-butanol after cooling, and the n-butanol extract was back-extracted with an alkaline aqueous solution with a pH value of 13 saturated with n-butanol, and the n-butanol solution after back extraction was adjusted to pH The value is 7, and the n-butanol solution is reclaimed to obtain the Flos Lonicerae extract, after testing, by weight, wherein Lonicera tomentosa saponin B (hereinafter referred to as component a): Lonicera tomentosa saponin A (hereinafter referred to as group Part b): 3-O-α-L-rhamnopyranosyl (1-2)-α-L-arabinopyranosyl-hederidenin (hereinafter referred to as component c): 3-O- β-D-glucopyranosyl(1-4)...

Embodiment 2

[0029] Red gland honeysuckle flower and flower buds, use 10 times the volume of water, extract at 60 degrees Celsius, recover the solvent to one-sixth of the original volume of the extract, cool to 2 degrees Celsius, filter to obtain the filtrate, add 50% NaOH to the filtrate, and add 50% NaOH to the filtrate. It was hydrolyzed for 3 hours under Celsius, extracted with n-butanol after cooling, and the n-butanol extract was back-extracted with 0.1% NaOH aqueous solution saturated with n-butanol, and the pH value of the n-butanol solution after the back extraction was adjusted to 7, and the n-butanol solution was recovered. The butanol solution obtains the Flos Japonica extract, and after testing, by weight, component a: component b: component c: component d=42:9:10:1, and the total weight of the four ingredients It accounts for 62% of the extract, and the yield is 12%.

Embodiment 3

[0031] The red glandular honeysuckle flower and flower buds and the yellow-brown honeysuckle flower and flower buds with a weight ratio of 1:1 are extracted at normal temperature with 100% methanol at a concentration of 3 times the volume, and the extract is recovered to a quarter of the original volume. Cool down to 4 degrees Celsius, filter to obtain the filtrate, add 0.5% KOH to the filtrate, hydrolyze it at 40 degrees Celsius for 10 hours, extract it with n-butanol, and extract the n-butanol extract with 0.8% KOH saturated with n-butanol. The final n-butanol solution adjusts the pH value to 7, reclaims the n-butanol solution and obtains the Flos Japonica extract, after testing, by weight, component a: component b: component c: component d=14:2:3:1 , and the total weight of the four components accounts for 74% of the extract, with a yield of 11%.

[0032] First mix 120 g of polyethylene glycol and 30 g of lactose, then add 300 g of the above-mentioned extract and mix evenly...

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Abstract

The invention relates to wild honeysuckle flower extract, which mainly comprises the following compositions: macranthoside A,II b, macranthoside B,IIIb, 3-O-alpha-L-pyran rhamnose (1-2)-alpha-L-pyran arabinose-hederagenin and 3-O-beta-D-glucopyranose (1-4)-beta-D-glucopyranose (1-3)-alpha-L-pyran rhamnose (1-2)-alpha-L-pyran arabinose -oleanolic acid. The invention also relates to a method for preparing the compound and application of the compound in the aspect of cancer.

Description

technical field [0001] The present invention relates to the field of traditional Chinese medicine pharmacy, in particular to an extract of Japonica japonica and its preparation method and application, specifically, it is the extract of effective active parts extracted from the traditional Chinese medicine Japonica japonica - total saponins, and researches have determined A method for preparing the extract with simplified process and high yield, and the application of the extract in preparing medicines for preventing and treating cancer. Background technique [0002] Lonicera plants are mostly deciduous or evergreen upright shrubs, dwarf shrubs and vines, rarely small trees. There are 47 recorded medicinal Lonicera plants, which have a strong antibacterial effect on many pathogenic bacteria. my country has recorded the medicinal value of Lonicera as early as 1,500 years ago. Its rhizomes, leaves, and flowers can be used as medicine. . Mountain silver flower is the dry flower...

Claims

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Application Information

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IPC IPC(8): A61K36/355A61K31/7048A61P35/00A61K133/00
Inventor 冯煦赵兴增贾晓冬陈雨王鸣印敏董云发
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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