Eupenicillium brefeldianum variety ZJB082702 and application thereof in preparation of Brefeldin A by fermentation

A technology of ZJB082702, brefeldin, applied to the field of application in fermentation preparation of brefeldin A

Inactive Publication Date: 2010-09-15
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few literature reports about the production and preparation of brefeldin A. U.S. Patent U.S. 3896002 discloses the preparation process of brefeldin A-producing strain Pseudomonas meliloti (P.medicaginis) and brefeldin A, P.medicaginis fermented at 25°C for 5 days, the concentration of brefeldin A reached 300mg / L

Method used

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  • Eupenicillium brefeldianum variety ZJB082702 and application thereof in preparation of Brefeldin A by fermentation
  • Eupenicillium brefeldianum variety ZJB082702 and application thereof in preparation of Brefeldin A by fermentation
  • Eupenicillium brefeldianum variety ZJB082702 and application thereof in preparation of Brefeldin A by fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Preparation of brefeldin A by fermentation of Penicillium brefeldin variant CCTCC No.208113

[0048] Inoculate the spore suspension of Penicillium breepenicillium CCTCC No.208113 into a 500ml Erlenmeyer flask filled with 160.0ml of seed medium, and culture it at 28°C for 2 days to obtain the seed solution. Seed medium preparation: maltose 30.0g, malt extract powder 1.5g, yeast extract powder 2.5g, potassium dihydrogen phosphate 2.0g, magnesium sulfate 2.0g, calcium carbonate 4.0g, distilled water 1000.0mL, pH7.0, seed medium 121 Sterilize at ℃ for 20 minutes;

[0049] In order to investigate the effect of the compound nitrogen source of the medium on the fermentation level of brefeldin A, five different fermentation mediums were designed, and the bioreactors used shake flasks with baffles, and the liquid volume was 160mL / 500mL shake flasks , the composition of the fermentation medium is respectively:

[0050] Medium I: Glucose 20.0g / L, Starch 10.0g / L, Malt ...

Embodiment 2

[0057] Example 2: Preparation of brefeldin A by fermentation of Penicillium brefeldin variant CCTCC No.208113

[0058] Inoculate the spore suspension of Penicillium breezii ZJB082702 (CCTCC No.208113) into a 500ml Erlenmeyer flask with 160.0ml of seed culture medium, and the seed culture medium is prepared as follows: 30.0g of maltose, 1.5g of malt extract powder, Yeast extract powder 2.5g, potassium dihydrogen phosphate 2.0g, magnesium sulfate 2.0g, calcium carbonate 4.0g, distilled water 1000.0mL, pH7.0, seed medium sterilized at 121°C for 20 minutes; cultivated at 28°C for 2 days to obtain seed liquid .

[0059] In order to investigate the influence of the amount of calcium carbonate in the culture medium on the fermentation level of brefeldin A, this patent designs 4 different fermentation culture mediums, and the bioreactor adopts shake flasks with baffles, and the liquid volume is 160mL / 500mL shake flask, the composition of the fermentation medium is as follows:

[00...

Embodiment 3

[0066] Example 3: Preparation of brefeldin A by fermentation of Penicillium brefeldin variant CCTCC No.208113

[0067] Inoculate the spore suspension of Penicillium breepenicillium CCTCC No.208113 into a 500ml Erlenmeyer flask containing 160mL of seed medium. The seed medium was prepared as follows: 30.0g of maltose, 1.5g of malt extract powder, and 2.5g of yeast extract powder. g, 2.0 g of potassium dihydrogen phosphate, 2.0 g of magnesium sulfate, 4.0 g of calcium carbonate, 1000.0 mL of distilled water, pH 7.0, sterilize the seed medium at 121° C. for 20 minutes; cultivate at 28° C. for 2 days to obtain the seed liquid.

[0068] In order to investigate the effect of the initial pH value of different culture media on the fermentation level of brefeldin A, this patent designs 4 different fermentation culture media, and the bioreactor adopts shake flasks with baffles, and the liquid volume is 160mL / 500mL shake flask, the composition of the fermentation medium is as follows: ...

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Abstract

The invention provides a Brefeldin A high-yield strain-Eupenicillium brefeldianum variety ZJB082702 and an application thereof in preparation of Brefeldin A by fermentation. The Eupenicillium brefeldianum variety ZJB082702 is collected in China Center for Typical Culture Collection, the address of the center: Wuhan University, Wuhan, China, 430072, the accession data is July 31, 2008, and the accession number is CCTCC No: M 208113. The use of the strain for preparing the Brefeldin A by fermentation has higher yield, and the maximum concentration of the Brefeldin A can achieve 943.8mg / L of fermentation broth.

Description

(1) Technical field [0001] The invention provides a high-yielding brefeldin A strain—Eupenicillium brefeldianum variety ZJB082702, and its application in the preparation of brefeldin A by fermentation. (2) Background technology [0002] Brefeldin A (Brefeldin A) is a naturally occurring macrolide antibiotic with the chemical name [(1R, 2E, 6S, 10E, 11as, 14aR)-1, 6, 7, 8, 9, 11a, 12, 13, 14, 14a-Decahydro-1, 13-dihydroxy-6-methyl-4H-cyclopent[f]oxacyclotridecin-4-one], the molecular formula is C 16 h 24 o 4 , the molecular weight is 280, and the structural formula is as follows: [0003] [0004] In 1958, Brefeldin A was isolated from Penicillium decumbens fermentation broth for the first time (Singleton et al., 1958). Early studies have found that brefeldin A inhibits the process of protein transport from the endoplasmic reticulum to the Golgi complex, and has antibacterial, antifungal and antiviral properties. Because brefeldin A affects protein transport and proce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P19/62C12R1/645
Inventor 郑裕国王亚军薛锋薛亚平沈寅初
Owner ZHEJIANG UNIV OF TECH
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