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Mistletoe polysaccharide, as well as preparation and use thereof

A technology of mistletoe and polysaccharides, applied in the field of polysaccharides

Inactive Publication Date: 2009-07-22
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on the polysaccharide structure of mistletoe has not been reported yet.

Method used

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  • Mistletoe polysaccharide, as well as preparation and use thereof
  • Mistletoe polysaccharide, as well as preparation and use thereof
  • Mistletoe polysaccharide, as well as preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Extraction, separation and purification, identification of embodiment 1.VCP

[0031] Weigh 500g of traditional Chinese medicine mistletoe powder, add 2500ml of water, extract in a water bath at 60°C for 2h, stir intermittently, filter the residue with gauze, centrifuge at 4000rpm to remove the precipitate, concentrate the extract to 500ml, add 3 times the volume of ethanol, place at 4°C for 12h, centrifuge, The precipitate was washed successively with absolute ethanol, acetone and ether, and freeze-dried to obtain crude VCP.

[0032] Dissolve the crude polysaccharide powder in 200ml of distilled water, centrifuge after fully dissolved, and remove the precipitate. Add an equal volume of Sevag solution (chloroform: n-butanol = 3:1), shake vigorously, let stand to separate layers, remove the lower organic layer and the middle layer, keep the aqueous layer, and extract twice. The resulting aqueous solution was dialyzed against distilled water for 24 hours, concentrated, an...

Embodiment 2

[0036] Extraction, separation and purification, identification of embodiment 2.VCP

[0037] Weigh 500g of traditional Chinese medicine mistletoe powder, add 1000ml of water, extract in a water bath at 70°C for 3h, stir intermittently, filter the residue with gauze, centrifuge at 4000rpm to remove the precipitate, concentrate the extract to 750ml, add 4 times the volume of ethanol, place at 4°C for 18h, centrifuge, The precipitate was washed successively with absolute ethanol, acetone and ether, and freeze-dried to obtain crude VCP.

[0038] Dissolve the crude polysaccharide powder in 500ml of distilled water, centrifuge after fully dissolved, and remove the precipitate. Add 3 times the volume of Sevag solution (chloroform: n-butanol = 4:1), shake vigorously, let stand to separate the layers, remove the lower organic layer and the middle layer, keep the aqueous layer, and extract repeatedly 3 times. The resulting aqueous solution was dialyzed against distilled water for 36 hou...

Embodiment 3

[0040] Extraction, separation and purification, identification of embodiment 3.VCP

[0041] Weigh 500g of traditional Chinese medicine mistletoe powder, add 5000ml of water, extract in 90°C water bath for 5h, stir intermittently, filter the residue with gauze, centrifuge at 8000rpm to remove the precipitate, concentrate the extract to 1000ml, add 5 times the volume of ethanol, place at 4°C for 24h, centrifuge, The precipitate was washed successively with absolute ethanol, acetone and ether, and freeze-dried to obtain crude VCP.

[0042] Dissolve the crude polysaccharide powder in 800ml of distilled water, centrifuge after fully dissolved, and remove the precipitate. Add 5 times the volume of Sevag solution (chloroform: n-butanol = 5:1), shake vigorously, let stand to separate layers, remove the lower organic layer and the middle layer, keep the aqueous layer, and extract repeatedly 5 times. The resulting aqueous solution was dialyzed against distilled water for 48 hours, conc...

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Abstract

A mistletoe polysaccharide consists of glucose, arabinose, galactose, glucuronic acid and galacturonic acid, with the molecular weight of 1.1 multiplied by 10-3.4 multiplied by 10Da, the terminal group carbon of Alpha-configuration, and the specific rotation of [Alpha]D being equal to plus 134.6 degrees to plus 167.3 degrees; and the basic skeleton of the polysaccharide consists of 1-5 glycosidic bond connected Arab sugar and 1-6 glycosidic bond connected galactose. The mistletoe polysaccharide is obtained by water extraction and alcohol precipitation, an ion exchange chromatography and a molecular sieve chromatography by further purification. The mistletoe polysaccharide has obvious inhibition action on Hela cells and mouse transplanted tumors S-180, so the polysaccharide can be used for preparing drugs for treating cancer.

Description

technical field [0001] The invention relates to a polysaccharide, in particular to a traditional Chinese medicine mistletoe polysaccharide Viscum coloratumpolysaccharide (VCP for short), its preparation method and application. Background technique [0002] Mistletoe (Viscum coloratum (Kom.) Nakai) is a semi-parasitic evergreen small shrub plant of Lorantheceae (Lorantheceae), also known as holly, northern parasitic, willow parasitic, etc. Mistletoe is a traditional Chinese medicine in my country with a long history of application. Its dried stems and branches are the more commonly used traditional Chinese medicine "Mistletoe" recorded in the Chinese Pharmacopoeia, and listed as the top grade in "Shen Nong's Materia Medica". It is used to treat rheumatic arthralgia, soreness of waist and knees, numbness of lower limbs, fetal movement, threatened abortion and high blood pressure. [0003] Its close relative plant, ginkgo mistletoe, is a folk medicinal plant in Europe. Tens o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P35/00A61K31/715A61K36/185A61K135/00
Inventor 龚祝南盛晓玲吴子刚张宇思王伟
Owner NANJING NORMAL UNIVERSITY
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