Radix rauvolfia total alkaloids separation method in Radix rauvolfia extract

Abstract

The invention discloses a method for separating total alkaloid from traditional Chinese medicine, in particular a method for separating total alkaloid from an extracting solution of the traditional Chinese medicine, namely devilpepper, and belongs to the field of traditional Chinese medicines. The method comprises the following technical proposal: taking the extracting solution of the devilpepper; adjusting the pH value of the extracting solution to between 1 and 7; filtering the extracting solution; taking and adding the filtrate to a cation exchange resin column; rinsing the filtrate and removing the impurities; eluting the filtrate with 0.5 to 15 percent acid liquor; collecting eluent; adding alkali to the eluent for neutralization; performing desalting treatment on the mixed solution; and performing concentrated drying to obtain the devilpepper total alkaloid. The method overcomes the defects of low extracting rate, large using level of organic solvent, complicated process, inability of large-scale production, and the like in the prior art and can efficiently separate high purity of the devilpepper total alkaloid from the extracting solution of the devilpepper.

Description

technical field [0001] The invention relates to a method for extracting and separating active ingredients of traditional Chinese medicine, in particular to a method for extracting and separating total alkaloids of Rauwolfia oleifera from the extract liquid of traditional Chinese medicine Rauwolfia, belonging to the field of traditional Chinese medicines. Background technique [0002] Alkaloids are a large class of basic nitrogen-containing compounds that widely exist in nature, are active ingredients of many Chinese herbal medicines, and are an important class of substances in nature. Rauvolwolfia is the root of Rauvolwolfia Rauvolfiaverticillata (Lour.) Baill. and Yunnan Rauwolfia R.yunnanensis Tsiang. A commonly used folk Chinese herbal medicine, which has the effects of promoting blood circulation and relieving pain, clearing heat and detoxifying, calming and lowering blood pressure. Studies have shown that the total alkaloids of Rauwolfia are its main effective parts, i...

AI Technical Summary

Problems solved by technology

Although this method can improve the purity of alkaloids, it has a certain degree of progress, but there are still serious defects: the test found that the efficiency of alkalizing the resin with ammonia water was low, resulting in a low yield; Wood alkaloids need to have good solubility in organic solvents, but the use of organic solvents will bring residues of resin monomer components such as styrene, divinylbenzene, and organic solvents into the eluent, which will affect the safety of the preparation; During the test, the resin needs to be poured out and dried, then alkalized with ammonia water, and then refluxed with various organic solvents for extraction. The steps are cumbersome, and pipeline production cannot be realized. At the same time, due to the use of organic solvents in the process, the pretreatment of the resin must be Increase the steps of organic solvent treatment such as ethanol, and recover the organic solvent later, which increases the process steps and increases the production cost