Calibration method for CCD camera imaging application

Abstract

The invention provides a calibration method for CCD camera imaging application, which comprises a calibration method for the pixel-response nonuniformity of a CCD camera, and a calibration method for reversely calculating the absolute intensity of a corresponding light source by utilizing the known absolute magnitude of an integrating sphere uniform light source and the pixel gray of an image captured by the corresponding CCD camera, wherein the calibration method for pixel-response nonuniformity automatically obtains a compensation matrix of a full-width image according to the gray information of each pixel on the image, and the calibration method for absolute intensity reversely calculates the quantitative information of the functional relation of the absolute intensity of the corresponding light source through the exposure time of the image and the gray of each pixel on the image. The calibration method for pixel-response nonuniformity and the calibration method for absolute intensity can follow the changes of the exposure time of the CCD camera and the pixel gray of the image to automatically calibrate pixel-response nonuniformity and the absolute intensity of the corresponding light source according to the calibration functional relation obtained in the calibration method.

Description

technical field [0001] The invention relates to the technical fields of optical imaging technology, image processing and data analysis, and in particular to the technical field of data analysis and processing of a calibration method for optical CCD camera imaging. Background technique [0002] At present, in the imaging of CCD cameras in scientific research such as astronomy and biological imaging, it is hoped that each pixel imaged by the CCD camera is also uniformly responsive under a uniform light source, that is, the response of the pixel of the CCD camera to the same irradiance is consistent. Not only that, but also hope that the pixel grayscale imaged by the CCD camera can accurately reflect the irradiance value of the corresponding light source under a surface light source with a certain irradiance, especially in the past two years in the research of bio-optical molecular imaging. Quantitative research is needed In in vivo imaging, the changes in the level of cells i...

AI Technical Summary

Problems solved by technology

In the aforementioned two U.S. patents, in order to obtain different compensation factors under different exposure times, a large number of experiments are required. It is to calibrate a single image captured by the camera, which cannot be changed when the exposure time changes and the grayscale of the image pixels also changes. to calibrate

Very inconvenient in the actual compensation process

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