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Methods of screening for agents that inhibit binding between mphosph1 and prc1

An agent and binding activity technology, applied in the field of screening agents that inhibit the binding between MPHOSPH1 and PRC1, can solve the problems of large side effects and poor overall prognosis

Inactive Publication Date: 2009-10-14
ONCOTHERAPY SCI INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the overall prognosis remains poor
Moreover, the side effects of M-VAC chemotherapy are extremely high (Vaughn DJ, Semin Oncol 1999, 26(suppl 2): ​​117-22)

Method used

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  • Methods of screening for agents that inhibit binding between mphosph1 and prc1
  • Methods of screening for agents that inhibit binding between mphosph1 and prc1
  • Methods of screening for agents that inhibit binding between mphosph1 and prc1

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Experimental program
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Effect test

Embodiment

[0152] 1. Materials and Methods

[0153] (1) Bladder cancer cell lines and tissue samples

[0154] Human bladder cancer cell lines HT1197, UM-UC-3, J82, HT1376, SW780 and RT4 were purchased from the American Type Culture Collection (ATCC; Rockville, MD). The bladder cancer cell lines COS7 and NIH3T3 cells used were all monolayer cultured in appropriate media, i.e. EMEM containing 0.1 mM essential amino acids (Roche), 1 mM sodium pyruvate (Roche) for HT1197, UMUC3, J82 and HT1376 ( Sigma, St. Louis, MO); L-15 was used for SW780; McCoy's 5a (sigma) was used for RT-4; Dulbecco's modified Eagle medium (Invitrogen, Carlsbad, CA) was used for COS7 and NIH3T3. Each medium was supplemented with 10% fetal bovine serum (Cansera) and 1% antibiotic / antifungal solution (Sigma). SW 780 cells in the absence of CO 2 maintained at 37°C in a humidified air atmosphere. Other cell lines in 5% CO 2 maintained at 37°C in a humidified air atmosphere.

[0155] Tissue samples of surgically r...

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Abstract

The present invention provides a method of identifying agents that inhibit or suppress the binding between MPHOSPH1 and PRC1. Such agents are expected to suppress the proliferation of cell expressing MPHOSPH1 and PRC1, and function as agents to prevent or treat cancer, such as bladder cancer.

Description

[0001] This application claims the benefit of US Provisional Patent Application No. 60 / 840,124, filed August 25, 2006, which is hereby incorporated by reference in its entirety. field of invention [0002] The invention relates to a screening method using the combination of M phase phosphoprotein (MPHOSPH1) and cytoplasmic division protein protein regulatory factor 1 (PRC1) as an index. The identification of an agent suitable for the treatment or prevention of cancer, especially bladder cancer, can be carried out according to the present method. Background technique [0003] Bladder cancer is the second most common genitourinary tumor in the population, with approximately 357,000 new cases worldwide each year (Parkin DM et al., CA Cancer J Clin 2005, 55:74-108). About 1 / 3 of these patients are likely to have invasive disease or metastatic disease at the time of diagnosis (Parkin DM et al., CA Cancer J Clin 2005, 55:74-108; Sternberg CN, Ann Oncol 1995, 6: 113-26; Ardavanis ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N2500/02G01N33/57407A61P13/10A61P35/00
Inventor 中村佑辅片桐丰雅中鹤修一
Owner ONCOTHERAPY SCI INC