Supercharge Your Innovation With Domain-Expert AI Agents!

Methods of disrupting quorum sensing to affect microbial population cell density

A technology of quorum sensing and quorum sensing signals, applied in botany equipment and methods, biofuels, bacteria, etc., can solve the problem that microorganisms cannot be cultivated

Inactive Publication Date: 2009-10-28
ATHENA BIOTECH INC
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] [0015] Certain microorganisms are known to utilize quorum sensing to control their cell division, and therefore, many microorganisms cannot be cultured in the laboratory due to quorum sensing

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Methods of disrupting quorum sensing to affect microbial population cell density
  • Methods of disrupting quorum sensing to affect microbial population cell density
  • Methods of disrupting quorum sensing to affect microbial population cell density

Examples

Experimental program
Comparison scheme
Effect test

experiment Embodiment 1

[0242] Experimental Example 1: Genetic Modification

[0243] Ethanol Production Strategies

[0244] [0185] The maximum cell density achieved in Zymomonas mobilis growth is influenced by type 1 quorum sensing using AHL signaling molecules. Heterologous expression of AHL-degrading enzymes, e.g., from Pseudomonas aeruginosa (P. aeruginosa) PvdQ (nucleotide sequence SEQ ID NO: 3 and amino acid sequence SEQ ID NO: 4) or AiiA (nucleotide sequence SEQ ID NO: 5 and amino acid sequence SEQ ID NO: 6) from Bacillus cereus (B. cereus) ). However, any AHL-acyltransferase or AHL-lactonase that alters or degrades an AHL substrate can be used for the same purpose. Zymomonas mobilis strains grown to higher densities are commercially valuable, for example in the fermentation of ethanol from sugars, as the volumetric productivity of the reactor increases in direct proportion to the cell density of the fermenting organism cells.

[0245] Identification of LuxR-type proteins in Zymomonas...

experiment Embodiment 2

[0268] Experimental Example 2: Exogenous Reagents

[0269] Cloning and expression of AHL-degrading enzyme in Zymomonas mobilis

[0270] [0197] Two AHL-degrading enzymes, each representing a known enzymatic AHL-degrading mechanism, were expressed in Zymomonas mobilis to disrupt quorum sensing and allow the organism to grow to higher cell densities. The selected enzymes were AiiA AHL-lactonase from Bacillus cereus and PvdQ AHL-acyltransferase from Pseudomonas aeruginosa.

[0271] [0198] The broad host range plasmid pAB300 as described above was used to express the two enzymes. Using standard molecular biology techniques, the genes encoding AiiA and PvdQ were cloned downstream of the tac promoter in pAB300, resulting in plasmids pAB310 and pAB320, respectively. Clones were constructed in E. coli and subsequently transferred into Zymomonas mobilis by electroporation. Containing 200μgml -1 Transformants were selected and isolated on kanamycin rich medium (RM) agar plates. ...

experiment Embodiment 3

[0281] Experimental Example 3: Screening for "knockout" mutations imparting growth effects

[0282] [0205] The following experiments were designed to screen for genes responsible for the generation or detection of quorum sensing signals. Inactivation of the quorum sensing system is not believed to have a lethal effect.

[0283] [0206] Random mutagenesis is the preferred strategy for a number of reasons. Recovered mutations are thought to disrupt enzymes involved in quorum sensing; however, any genetic alteration that confers increased growth can be recovered. Disruption of genes in non-quorum sensing systems may also provide a growth advantage, and identification of these systems may not be predictable by other means. Random mutagenesis is expected to disrupt all genes in a chromosome and thus should identify any useful genes that would be predicted by sequence analysis. In addition, mutagenesis (eg, insertional mutagenesis) can identify regulatory DNA elements, thereby p...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to the modulation of quorum sensing mechanisms in a microorganism for the purpose of exploiting the fermentation capabilities of the microorganism.

Description

Background of the invention [0001] [0001] It has been shown that several genera of bacteria communicate with each other to adjust the expression of specific genes in a cell density-dependent manner. This bacterial communication is called quorum sensing, and it enables bacteria to control gene expression in response to levels of diffusible signaling molecules known as autoinducers. Quorum sensing systems rely on the constitutive, low-level expression of autoinducer molecules, which trigger the expression of a specific set of genes when their concentration in solution reaches a certain threshold level. This threshold is reached when sufficient numbers ("populations") of bacteria are present in a localized area such that the combined rate of autoinducer degradation and diffusive dilution is less than its rate of production (see, e.g., U.S. Patent Application Publication No. 20040038374, adopted herein fully incorporated herein by reference). Typically, signaling molecules bind ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/02A01N63/00
CPCC12N1/20Y02E50/17C12P7/06Y02E50/10
Inventor B·马尔斯B·M·斯瓦拉
Owner ATHENA BIOTECH INC
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com