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Biological preparation capable of preventing and treating rice streak disease and promoting growth and application thereof
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A technology of biological agents and rice, applied in the direction of chemicals, biocides, and applications for biological control, can solve problems such as social pollution, environmental pollution, and chemical pesticide residues, and achieve efficient disease control and easy to use Effect
Inactive Publication Date: 2012-03-07
YUNNAN AGRICULTURAL UNIVERSITY
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Problems solved by technology
For example, the main medicaments for the prevention and treatment of bacterial diseases such as rice stripe disease in my country are organocopper fungicides (Lunke bacteria, thiasen copper, etc.) In addition, the long-term and large-scale use of this type of chemical agent has caused pathogenic bacteria to develop resistance to this type of agent.
In addition, due to the abuse of chemical pesticides, chemical pesticides have long-term residues in nature and the food chain, causing serious consequences such as environmental pollution, ecological balance damage, and social pollution.
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Embodiment 1
[0059] Embodiment one: the drug efficacy test of biological agent (YFY02) to rice bacterial stripe disease
[0060] (1) In vitro antibacterial test of biocontrol strains
[0061] 1. Test materials:
[0062] 1.1 Test strains Antibiotic Lysobacter strain YFY02 (Lysobacter ginsengisoli).
[0063] 1.2 Pathogens tested
[0064] Bacteria: Xathomonas oryzae pv. oryzicola, Xanthomonas oryzae pv. oryzae, P. syringae pv. tabaci and Xanthomonas axonopodis pv. .dieffenbachiae), the above pathogenic bacteria were provided by our laboratory.
[0065] 1.3 Reagents and media for testing
[0066] Ethyl acetate, acetone, methanol, petroleum ether, etc. are all analytically pure. R 2 A medium (yeast extract 0.50g; interstitial peptone 0.50g; acid hydrolyzed casein 0.50g; glucose 0.50g; soluble starch 0.50g; sodium pyruvate 0.30g; potassium hydrogen phosphate 0.30g; magnesium sulfate heptahydrate 0.05 g; distilled water 1000.00mL, pH7.2), PDA medium (potato 200g, agar 15g, glucose 15g, wat...
Embodiment 2
[0126] Embodiment two: biocontrol strain YFY02 handles the growth-promoting effect on rice seedlings
[0127] 1. The preparation and processing method of the bacteria solution:
[0128] First transfer the biocontrol bacteria to NA medium for activation, then streak and expand culture for 36-48h (28°C), wash the biocontrol bacteria from the solid medium with sterile water to make a bacterial suspension, and shake on the shaker Evenly, use a spectrophotometer to lower the OD value to 0.5 at a wavelength of 600nm, and the concentration of the bacterial suspension is equivalent to (3×10 8 CFU / ml), the YFY02 biocontrol bacteria solution with an OD value of 0.5 was 10 times (3×10 7 cfu / mL), 100 times (3×10 6 cfu / mL), 200 (1.5×10 6 cfu / mL) times, 500 (6×10 5 cfu / mL) times, 1000 (3×10 5 cfu / mL) times concentration gradient dilution, respectively for seed soaking treatment. Soak the seeds for 48 hours, and replace the treatment solution every 12 hours. The rice material is yello...
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Abstract
The invention relates to a biological preparation capable of preventing and treating rice streak disease and promoting growth and application thereof, belonging to the technical field of biological pesticide. The biological preparation is prepared by a conventional method, and a producing strain is YFY02 (Lysobacter ginsengisoli). The strain of the biological preparation is characterized in that (1) the colony is round and in a opaque dilute liquid shape, and is dark yellow at initial stage of culture and chocolate brown or dark brown at later stage of culture, and the thallus is in the shapeof a long rod; (2) the strain has strong bacteriostatic ability and wide antibacterial spectrum, can keep value on leaf surfaces of crops such as rice and the like and roots of crops such as konjak, calla and the like, can generate substances similar to antibiotics to promote the growth of crops of rice and the like, and has obvious and stable effect on preventing and treating rice streak disease. In the invention, the strain is cultured in liquid or solid and is prepared to liquid preparation or wettable powder. The invention has obvious and stable effect on preventing and treating bacterialdiseases of rice streak disease, and good comprehensive properties, and is easy for industrial production.
Description
Technical field: [0001] The invention relates to a biological preparation for preventing and treating rice bacterial streak disease and an application thereof, belonging to the technical field of biological pesticides. Background technique: [0002] Rice bacterial streak (Xanthomonoas oryzae pv. oryzicola), referred to as streak disease, is widely distributed in tropical and subtropical regions of Asia. It is mainly distributed in South China, Central China, Southwest China and other regions in my country. It is the fourth largest rice disease after bacterial blight, and it is also an important quarantine disease of rice in my country. In recent years, due to the large-scale promotion of susceptible rice varieties, especially hybrid rice, southern propagation and seed transfer, the disease has gradually spread and spread, and the disease area has continued to expand. It has become one of the main diseases in southern rice areas in my country. When the disease occurs, it spre...
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Application Information
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