Pseudovirus based on Vasohibin gene and preparation method and application thereof

A pseudovirus and gene technology, applied in the field of genetic engineering, can solve problems such as unfavorable efficient transfection of target genes, and achieve the effects of inhibiting tumor angiogenesis, improving safety, and inhibiting adhesion and migration.
CN101624581AInactive Publication Date: 2010-01-13ARMY MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN ยท China
Current Assignee / Owner
ARMY MEDICAL UNIV
Publication Date
2010-01-13
Estimated Expiration
Not applicable ยท inactive patent

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Abstract

The invention discloses pseudovirus based on Vasohibin gene and a preparation method and application thereof. The pseudovirus is a substance simulating a natural virus structure, which is obtained in such a way that viral capsid chimeric protein HPV16L1-RGD wraps a Vasohibin gene recombination eukaryotic expression vector, wherein the HPV16L1-RGD is formed by inserting RGC peptide between the 266th amino acid and the 267th amino acid of the HPV16L1. The preparation method of the pseudovirus comprises three steps: constructing the HPV16L1-RGD, constructing the Vasohibin gene recombination eukaryotic expression vector and wrapping the Vasohibin gene recombination eukaryotic expression vector with the HPV16L1-RGD. The pseudovirus can escape from organic cleaning mechanism at the maximum, be exclusively absorbed by new vascular endothelial cells of a tumor and tumor cells, play the roles of inhibiting tumor neovascularization and inducing the apoptosis of the tumor cells, and can be used for preparing antineoplastic.
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Description

technical field

[0001] The invention relates to the field of genetic engineering, in particular to a pseudovirus based on Vasohibin gene and its preparation method and application. Background technique

[0002] Tumor is a disease with high morbidity and high mortality that seriously threatens human health. After surgery, radiotherapy and chemotherapy, biological therapy has become an important means in the comprehensive treatment of tumors. At present, tumor biological treatment strategies include targeted therapy of tumor-related genes or tumor suppressor genes, immunotherapy of tumor-associated antigens, etc., but due to technical maturity, effectiveness, safety and economics, etc., they are restricted in practical application. Very restrictive.

[0003] Studies have found that more than 90% of human tumors are solid tumors, and the growth and metastasis of solid tumors require angiogenesis, and inhibiting angiogenesis can effectively inhibit tumor growth and metastasis ...

Claims

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