30 results about "Virus Structure" patented technology

The invention concerns agents for the treatment of acute and chronic infections with human and animal pathogenic viruses which assemble along the cell membrane and are released through budding on the surface of the cell. Hereunto count especially causative agents of infectious diseases such as AIDS, hepatitis, hemorrhagic fever, SARS, smallpox, measles, polio or the flu. The subjects of the invention are agents that contain inhibitors of the protein folding as active components. Hereunto count inhibitors of cellular folding enzymes (the enzymatic chaperones) as well as substances that disturb the folding of proteins through chemical chaperones. The following substance classes and their derivates belong thereunto: Geldanamycin, Deoxyspergualin, 4-PBA or Herbimycin A. Due to these agents the highly organised processes of the assembly and the proteolytical maturation of virus structure proteins is disturbed. As a result the release and production of infectious decendent viruses is prevented.

The invention belongs to a self-conversion type multipurpose mask and a conversion using method thereof. The self-conversion type multipurpose mask is composed of a left butt joint barrel, a right butt joint barrel, a compression spring, a valve block, an adjusting screw ring, an air outlet filter box, an air inlet filter box and a filter material. And the filter material is a fiber sheet, an activated carbon mixed adsorbing material or a sterilization mixed adsorbing material. According to the method, the structure of the mask is changed according to the purposes of a user, and the purposes comprise a single-port dustproof structure, a double-port dustproof structure, a one-way dustproof and anti-virus structure, a double-port dustproof and anti-virus structure and a two-way dustproof andanti-virus structure. According to the purposes of the mask, the structure of the mask can be changed rapidly in a self-service mode, the mask can be changed rapidly in a self-service mode and has the two-way anti-virus and dustproof effects, and the mask has the advantages of being simple in structure, multipurpose, convenient to use and disinfect and good in effect.

The preparation process of reovirus antigen subunit vaccine for grass carp includes the following steps: proliferation of GCRV873, which is the Hunan Shaoyang strain of reovirus, in grass carp kidney cell line; centrifugal purification and concentration of GCRV873 infected cell virus suspension; processing purified virus with surfactant to disintegrate complete virus structure to form viral nucleic acid and capsid protein subunit component; digesting with nuclease to degrade free virus genome dsRNA; dialysis to obtain reovirus protein subunit antigen preparation for grass carp containing no nuclease; and diluting with physiological saline to obtain the said vaccine. The vaccine of the present invention has the advantages of containing complete virus protein antigen component, containing no genetic virus matter RNA, simple preparation process, high product purity, etc.

The invention discloses a preparation method of an orally-taken vaccine for treatment of hemorrhage of a grass carp, which comprises the following steps of: (1) preparing a recombinant baculovirus BmNPV-IIVP6 with hemorrhagic virus structure protein VP6 genes of the grass carp in a genetic engineering method; (2) grafting the amplified recombinant baculovirus BmNPV-IIVP6 to a five-instar silkworm larvae or pupae; (4) collecting the silkworm larvae or pupae subjected to virus inoculation after 4 to 6 days, and preparing freeze-dried powder in a freeze-drying method; and (4) mixing the freeze-dried powder prepared in Step (3) and powdered fish feed, to obtain the orally-taken vaccine for treatment of hemorrhage of the grass carp. The content of the freeze-dried powder in the orally-taken vaccine for treatment of hemorrhage of the grass carp is 1-10 percent by weight. The vaccine can be directly used as an orally-taken vaccine, and has the advantages of simple preparation process, low cost, convenient use and dual functions of protein subunit vaccines and nucleic acid vaccines.

The invention belongs to the field of high polymer materials and discloses a virus structure-imitated high-polymer vesica as well as a preparation method and application thereof. The virus structure-imitated high-polymer vesica is prepared from different amphipathic block copolymers through self-assembling or is prepared from amphipathic block copolymers and water-soluble polymers through self-assembling. The virus structure-imitated high-polymer vesica comprises a capsid membrane structure and a spike structure on the surface of a membrane, wherein the inner layer and the outer layer of the capsid membrane structure consist of hydrophilic chain segments in the amphipathic block copolymers or the water-soluble polymers, and an intermediate layer between the inner layer and the outer layer consists of hydrophobic chain segments in the amphipathic block copolymers. The high-polymer vesica has a virus-imitated specific structure and is controllable in structure, high in repeatability, capable of carrying drugs, good in biocompatibility and easy to be phagocytized by cells; the operation of the method is simple, and the production efficiency is high; and meanwhile, the high-polymer vesica can be applied to the relevant fields of high-efficiency nano-carriers, controlled release of medicines and cell imaging.

The invention discloses pseudovirus based on Vasohibin gene and a preparation method and application thereof. The pseudovirus is a substance simulating a natural virus structure, which is obtained in such a way that viral capsid chimeric protein HPV16L1-RGD wraps a Vasohibin gene recombination eukaryotic expression vector, wherein the HPV16L1-RGD is formed by inserting RGC peptide between the 266th amino acid and the 267th amino acid of the HPV16L1. The preparation method of the pseudovirus comprises three steps: constructing the HPV16L1-RGD, constructing the Vasohibin gene recombination eukaryotic expression vector and wrapping the Vasohibin gene recombination eukaryotic expression vector with the HPV16L1-RGD. The pseudovirus can escape from organic cleaning mechanism at the maximum, be exclusively absorbed by new vascular endothelial cells of a tumor and tumor cells, play the roles of inhibiting tumor neovascularization and inducing the apoptosis of the tumor cells, and can be used for preparing antineoplastic.