30 results about "Virus Structure" patented technology

The invention concerns agents for the treatment of acute and chronic infections with human and animal pathogenic viruses which assemble along the cell membrane and are released through budding on the surface of the cell. Hereunto count especially causative agents of infectious diseases such as AIDS, hepatitis, hemorrhagic fever, SARS, smallpox, measles, polio or the flu. The subjects of the invention are agents that contain inhibitors of the protein folding as active components. Hereunto count inhibitors of cellular folding enzymes (the enzymatic chaperones) as well as substances that disturb the folding of proteins through chemical chaperones. The following substance classes and their derivates belong thereunto: Geldanamycin, Deoxyspergualin, 4-PBA or Herbimycin A. Due to these agents the highly organised processes of the assembly and the proteolytical maturation of virus structure proteins is disturbed. As a result the release and production of infectious decendent viruses is prevented.

A self-service respiratory vaccination instrument for SARS infectious disease belongs to respiratory tract communicable disease treating, defending and spread proof medical apparatus. The self-service respiratory vaccination instrument uses instruments means to create high temperature as in summer, and uses the penetrating power of damp-heat water vapour, or the destructive power to the virus structure from negative oxygen ion and pH regulating agent, or the killing power to the microorganism from plant essence oil to make the respiratory tract virus, pathogenic bacteria such as 'SARS' to be attenuated, killed or inactivated; the microorganism attenuated, killed or inactivated in the respiratory tract and its giblets become specificity vaccine for the microorganism and are disposed in the respiratory tract, the effect that the 'SARS' respiratory tract microorganism vaccine can be inoculated to the respiratory tract with no time difference and no other outer vaccine can be realized, and the object of the cure, defense and diffuse proof for corresponding respiratory tract infectious diseases can be achieved.

The preparation process of reovirus antigen subunit vaccine for grass carp includes the following steps: proliferation of GCRV873, which is the Hunan Shaoyang strain of reovirus, in grass carp kidney cell line; centrifugal purification and concentration of GCRV873 infected cell virus suspension; processing purified virus with surfactant to disintegrate complete virus structure to form viral nucleic acid and capsid protein subunit component; digesting with nuclease to degrade free virus genome dsRNA; dialysis to obtain reovirus protein subunit antigen preparation for grass carp containing no nuclease; and diluting with physiological saline to obtain the said vaccine. The vaccine of the present invention has the advantages of containing complete virus protein antigen component, containing no genetic virus matter RNA, simple preparation process, high product purity, etc.

The invention belongs to the technical field of prevention and treatment on novel coronavirus and particularly discloses a method and equipment for effectively deactivating viruses. The method comprises the steps: S1. carrying out charging treatment on aerosol possibly containing pathogens; S2. applying an external electric field to the charged aerosol, so as to collect the aerosol in the presenceof an electric field force; and S3. carrying out virus killing treatment on the collected aerosol to destroy RNA structures of the viruses in the aerosol, thereby effectively deactivating the viruses. According to the method and the equipment, charged high-voltage line poles charge the aerosol possibly containing the pathogens through a pulsed high-voltage power supply, the external electric field is applied to the charged aerosol to collect the charged aerosol in the presence of the electric field force, meanwhile, the collected aerosol is irradiated with powerful ultraviolet radiation to destroy the RNA structures of the viruses in the aerosol and enable the pathogens in the aerosol to lose reproduction capability or activity, and thus, the viruses are effectively deactivated.

The invention particularly relates to an enveloped ribonucleic acid (RNA) virus nucleic acid detection reference / standard platform. The enveloped RNA virus nucleic acid detection reference / standard platform is mainly prepared by using a vector on which an exogenous target sequence is carried, a vector containing an MLV gag-pol component, and a plasmid which comprises or does not comprise a viral membrane protein to cotransfect a reference producing cell strain, is a similar virus of an enveloped RNA virus, simulates a phospholipid bimolecular layer on the surface of the virus or further simulates the surface activity of the viral membrane protein, has the characteristics of concrete structure and physical and chemical properties of the enveloped RNA virus, and has no replication capacity and infectivity. An enveloped RNA virus nucleic acid detection reference / standard is packaged from eukaryocytes by a recombinant virus technology; the platform can ensure that an exogenous target RNA sequence is carried inside reference / standard particles by a genetic manipulation technology; and the reference / standard has a virus envelope protein which is the same as that of a target virus as well as the viral structural characteristics and topological characteristics which are close to those of the target virus, and can truly reflect the influence of various physical and chemical environment factors on the target virus.

The invention belongs to the field of high-polymer materials and discloses an imitated virus-structured high-polymer vesicle with a target function and preparation and application thereof. The preparation method of the imitated virus-structured high-polymer vesicle with the target function comprises, S1, performing amide reaction on hyaluronic acid containing amino and hydrophobic polymers containing carboxyl to obtain hyaluronic acid grafted amphiphilic block copolymers, wherein the hydrophobic polymers containing carboxyl requires carboxyl activation before reaction are performed, and the hyaluronic acid containing amino are obtained through reaction between diamine and hyaluronic acid; S2, reacting the hyaluronic acid grafted amphiphilic block copolymers with other amphiphilic block copolymers through a solvent exchange method or a hydration method to obtain the imitated virus-structured high-polymer vesicle with the target function. The preparation method of the imitated virus-structured high-polymer vesicle with the target function is simple in operation, flexible in modification, high in production efficiency and good in repeatability; the prepared imitated virus-structured high-polymer vesicle achieves the target function and can be applied to the biomedical fields such as drug carriers.

The invention belongs to the field of high polymer materials and discloses a virus structure-imitated high-polymer vesica as well as a preparation method and application thereof. The virus structure-imitated high-polymer vesica is prepared from different amphipathic block copolymers through self-assembling or is prepared from amphipathic block copolymers and water-soluble polymers through self-assembling. The virus structure-imitated high-polymer vesica comprises a capsid membrane structure and a spike structure on the surface of a membrane, wherein the inner layer and the outer layer of the capsid membrane structure consist of hydrophilic chain segments in the amphipathic block copolymers or the water-soluble polymers, and an intermediate layer between the inner layer and the outer layer consists of hydrophobic chain segments in the amphipathic block copolymers. The high-polymer vesica has a virus-imitated specific structure and is controllable in structure, high in repeatability, capable of carrying drugs, good in biocompatibility and easy to be phagocytized by cells; the operation of the method is simple, and the production efficiency is high; and meanwhile, the high-polymer vesica can be applied to the relevant fields of high-efficiency nano-carriers, controlled release of medicines and cell imaging.

The invention discloses an indirect enzyme-linked immunosorbent assay (ELISA) kit for detecting a human West Nile virus and a detection method thereof. West Nile viruslike particles expressed by a mammal cell expression system provide an ELISA method for coating an antigen; and the viruslike particles (VLPs) are hollow particles comprising one or more virus structure protein, are nucleic acid without viruses, and has conformation and antigenic epitope as same as those of a natural virus, so the West Nile viruslike particles have no infectivity to a human body, high stability, high safety and immunogenicity closer to that of the natural virus, and is difficult to inactivate. A kit with safety, specificity, sensitivity, quickness, simpleness in operation and economy is provided for detecting a human West Nile virus IgG antibody; and a technical method is provided for preventing and controlling importation of the West Nile virus from foreign countries to China.

The invention discloses a card for rapidly and quantitatively detecting a pseudorabies virus antibody, and a use method thereof. The card comprises a detection card shell and a test strip assembled inthe detection card shell; the test strip comprises a plastic bottom plate with pressure-sensitive adhesive; a sample pad, a marker pad, a nitrocellulose film and water-absorbing paper are adhered tothe bottom plate sequentially; the marker pad consists of a carrier base layer and a marker; the marker is one layer of film formed by spray-coating the carrier base layer with a lanthanide fluorescence detection microsphere and a lanthanide fluorescence quality control microsphere; the nitrocellulose film is coated with a pseudorabies virus recombinant antigen serving as a detection line and coated with a rabbit anti-chicken IgY antibody serving as a quality control line; and the marker is a fluorescence detection microsphere marked with pseudorabies virus structure protein gB and gI (or gE)recombinant antigens and a fluorescence quality control microsphere marked with a chicken IgY antibody. The card can detect the pseudorabies antibody on site rapidly and quantitatively, and has higherpractical value and popularization value.

The invention discloses a preparation method of an orally-taken vaccine for treatment of hemorrhage of a grass carp, which comprises the following steps of: (1) preparing a recombinant baculovirus BmNPV-IIVP6 with hemorrhagic virus structure protein VP6 genes of the grass carp in a genetic engineering method; (2) grafting the amplified recombinant baculovirus BmNPV-IIVP6 to a five-instar silkworm larvae or pupae; (4) collecting the silkworm larvae or pupae subjected to virus inoculation after 4 to 6 days, and preparing freeze-dried powder in a freeze-drying method; and (4) mixing the freeze-dried powder prepared in Step (3) and powdered fish feed, to obtain the orally-taken vaccine for treatment of hemorrhage of the grass carp. The content of the freeze-dried powder in the orally-taken vaccine for treatment of hemorrhage of the grass carp is 1-10 percent by weight. The vaccine can be directly used as an orally-taken vaccine, and has the advantages of simple preparation process, low cost, convenient use and dual functions of protein subunit vaccines and nucleic acid vaccines.

The invention provides a reagent composition used for preserving the nucleic acid integrity of viruses in body fluid, and application and a method of the reagent composition. The reagent composition comprises urea, lauryl sodium sulfate, trans-1.2-cyclohexanediamine tetraacetic acid, Tris-HCl, propyl p-hydroxybenzoate, diazolidinyl urea, sodium acetate, ethyl alcohol, saccharose, protease K and phenol red. After the above reagent composition and a liquid virus sample are evenly mixed, long-term preservation can be realized under a constant-temperature condition. The reagent composition and the preservation method provided by the invention can damage a virus structure to release the nucleic acid, the reagent composition contains ingredients capable of inhibiting the enzyme activity of the nucleic acid to avoid nucleic acid degradation, meanwhile, a buffering system capable of stably preserving the nucleic acid for a long term is provided, preservation time is long, and an applicable temperature range is wide.

The invention belongs to the field of pharmacy, and relates to application of wogonin in preparing medicine for resisting a herpes simplex virus. An experiment verifies that wogonin has a high activity of resisting the herpes simplex virus in an in-vitro experiment and can inhibit the expression of virus structure protein gD in mRNA transcription and the protein level; an in-vitro toxicity experiment of wogonin finds that the flavone type compound has low toxicity to cells of the human and African green monkeys and can obtain a high treatment index; therefore, wogonin has potential value as novel medicine resisting the herpes simplex virus and can be applied to preparing the medicine for resisting the herpes simplex virus.

The invention provides a Chinese bee cysticercus virus structure protein VP2 gene prokaryotic expression used for egg yolk antibody preparation. A Chinese bee cysticercus virus structure protein VP2 gene transformed through a rare codon obtained according to the preference of escherichia coli to a codon is shown in SEQ ID No.1. The Chinese bee cysticercus virus structure protein VP2 gene prokaryotic expression has the advantage that the Chinese bee cysticercus virus structure protein VP2 gene is optimized and transformed. The transformed VP2 gene is subjected to prokaryotic expression and can produce a large number of VP2 proteins, and an egg yolk antibody prepared through a VP2 immunized chicken can be used for preventing a Chinese bee cysticercus disease and is remarkable in curative effect.

The invention belongs to a self-conversion type multipurpose mask and a conversion using method thereof. The self-conversion type multipurpose mask is composed of a left butt joint barrel, a right butt joint barrel, a compression spring, a valve block, an adjusting screw ring, an air outlet filter box, an air inlet filter box and a filter material. And the filter material is a fiber sheet, an activated carbon mixed adsorbing material or a sterilization mixed adsorbing material. According to the method, the structure of the mask is changed according to the purposes of a user, and the purposes comprise a single-port dustproof structure, a double-port dustproof structure, a one-way dustproof and anti-virus structure, a double-port dustproof and anti-virus structure and a two-way dustproof andanti-virus structure. According to the purposes of the mask, the structure of the mask can be changed rapidly in a self-service mode, the mask can be changed rapidly in a self-service mode and has the two-way anti-virus and dustproof effects, and the mask has the advantages of being simple in structure, multipurpose, convenient to use and disinfect and good in effect.

The invention discloses a LMP-1 adeno related virus vector, constructing method and application thereof. The LMP-1 adeno related virus vector is adeno related virus vector obtained by substituting adeno related virus structure in adeno related virus vector with LMP-1 gene or mutation type gene thereof. The LMP-1 adeno related virus vector can convey LMP-1 gene of wild type or mutation type carried thereby into monocyte-macrophage-dendritic cell system, cells carried with these specific antigen gene can be used as effector cells to stimulate immune system. As proven by experiments, CTL infected by the rAAV and induced by DC can effectively inhibit malignant tumor cells or kill tumor cells in patient's body. Therefore, the LMP-1 adeno related virus vector or relevant products thereof can be used in preparation of medicament for nasopharyngeal carcinoma.

The invention discloses pseudovirus based on Vasohibin gene and a preparation method and application thereof. The pseudovirus is a substance simulating a natural virus structure, which is obtained in such a way that viral capsid chimeric protein HPV16L1-RGD wraps a Vasohibin gene recombination eukaryotic expression vector, wherein the HPV16L1-RGD is formed by inserting RGC peptide between the 266th amino acid and the 267th amino acid of the HPV16L1. The preparation method of the pseudovirus comprises three steps: constructing the HPV16L1-RGD, constructing the Vasohibin gene recombination eukaryotic expression vector and wrapping the Vasohibin gene recombination eukaryotic expression vector with the HPV16L1-RGD. The pseudovirus can escape from organic cleaning mechanism at the maximum, be exclusively absorbed by new vascular endothelial cells of a tumor and tumor cells, play the roles of inhibiting tumor neovascularization and inducing the apoptosis of the tumor cells, and can be used for preparing antineoplastic.

The invention relates to a chimeric broad-spectrum oncolytic adenovirus for multi-mechanism synergistic immunotherapy and an application thereof in tumor treatment. The oncolytic adenovirus has the characteristics of triple tumor targeting regulation mechanism, modification of three virus structure genes, chimeric combination of three serum type adenoviruses and loading of three types of anti-cancer immune genes, and can activate the inherent anti-cancer activity of various virus structure proteins; the tumor cell infection capability is improved while the situation that viruses escape from interception of pre-stored neutralizing antibodies of organisms and adhesion and uptake of hepatocytes can be guaranteed; and the effect of killing cancer cells is improved and enhanced through three types of anticancer immunomodulatory genes. According to the key technology, the design concept of mutual cooperation of multiple mechanisms is ingeniously preset in a genome, the immunosuppression barrier of a tumor microenvironment can be broken, and an obvious synergistic effect is achieved on immune checkpoint inhibitor treatment and immune cell treatment. The oncolytic adenovirus disclosed by the invention belongs to a brand new type of oncolytic adenovirus, realizes real multi-mechanism synergistic interaction, is good in targeting property and strong in anticancer activity, has broad spectrum, and can be independently or auxiliarily used for treating cancers, especially solid tumors.

The invention provides a DNA virus reference material and a preparation method and application thereof. The DNA virus reference material is recombinant viruses which are obtained by recombining coding genes of nucleocapsid protein in the DNA viruses into baculovirus to be expanded and purified. The DNA virus reference material prepared from baculovirus contains a virus structure and can serve as a parallel contrast in the sample processing and extracting process, and conduct quality control on the operation technology. In addition, the prepared reference material can conduct quality control on agents used in the sample processing process and provide powerful guarantees for effectiveness of experimental results.

The invention discloses an antibacterial and antiviral ferritic stainless steel and a preparation method thereof. According to the invention, multiform alloying silver elements and rare earth elements are distributed in a ferritic structure; the silver elements are naturally activated by the environment to form silver ions with different valence states, and the silver ions can act on bacteria and virus independently or in a combined mode; so that bacteria respiration is inhibited, or a virus structure is damaged; and therefore the bacteria and virus is killed; and the rare earth elements can increase the corrosion resistance of the steel and improve the antibacterial activity of the steel at the same time, and the requirements of kitchens and bathrooms, household appliances, household appliance parts, tableware, other public facilities and the like for stainless steel materials are met advantageously.

The invention discloses an LMP-2 recombinant adeno-associated virus vector and a construction method and an application thereof. The LMP-2 recombinant adeno-associated virus vector is obtained by replacing an adeno-associated virus structure gene in an adeno-associated virus vector with an LMP-2 gene or a mutant gene thereof. A wild type or mutant type LMP-2 gene carried by the LMP-2 recombinant adeno-associated virus vector can be transported in a monocyte-macrophage-dendritic cell line by the LMP-2 recombinant adeno-associated virus vector, and cells carrying specific antigen genes are usedto stimulate effector cells of an immune system. Experiments show that CTL induced by DC and infected by rAAV of the invention can effectively inhibit the growth of malignant tumor cells or kill tumorcells in the body of patients, and therefore, the LMP-2 recombinant adeno-associated virus vector of the invention or products related to the LMP-2 recombinant adeno-associated virus vector can be used for preparing antitumor drugs.

The preparation process of reovirus antigen subunit vaccine for grass carp includes the following steps: proliferation of GCRV873, which is the Hunan Shaoyang strain of reovirus, in grass carp kidney cell line; centrifugal purification and concentration of GCRV873 infected cell virus suspension; processing purified virus with surfactant to disintegrate complete virus structure to form viral nucleic acid and capsid protein subunit component; digesting with nuclease to degrade free virus genome dsRNA; dialysis to obtain reovirus protein subunit antigen preparation for grass carp containing no nuclease; and diluting with physiological saline to obtain the said vaccine. The vaccine of the present invention has the advantages of containing complete virus protein antigen component, containing no genetic virus matter RNA, simple preparation process, high product purity, etc.

The invention belongs to the field of high polymer materials and discloses a virus structure-imitated high-polymer vesica as well as a preparation method and application thereof. The virus structure-imitated high-polymer vesica is prepared from different amphipathic block copolymers through self-assembling or is prepared from amphipathic block copolymers and water-soluble polymers through self-assembling. The virus structure-imitated high-polymer vesica comprises a capsid membrane structure and a spike structure on the surface of a membrane, wherein the inner layer and the outer layer of the capsid membrane structure consist of hydrophilic chain segments in the amphipathic block copolymers or the water-soluble polymers, and an intermediate layer between the inner layer and the outer layer consists of hydrophobic chain segments in the amphipathic block copolymers. The high-polymer vesica has a virus-imitated specific structure and is controllable in structure, high in repeatability, capable of carrying drugs, good in biocompatibility and easy to be phagocytized by cells; the operation of the method is simple, and the production efficiency is high; and meanwhile, the high-polymer vesica can be applied to the relevant fields of high-efficiency nano-carriers, controlled release of medicines and cell imaging.

The invention discloses pseudovirus based on Vasohibin gene and a preparation method and application thereof. The pseudovirus is a substance simulating a natural virus structure, which is obtained in such a way that viral capsid chimeric protein HPV16L1-RGD wraps a Vasohibin gene recombination eukaryotic expression vector, wherein the HPV16L1-RGD is formed by inserting RGC peptide between the 266th amino acid and the 267th amino acid of the HPV16L1. The preparation method of the pseudovirus comprises three steps: constructing the HPV16L1-RGD, constructing the Vasohibin gene recombination eukaryotic expression vector and wrapping the Vasohibin gene recombination eukaryotic expression vector with the HPV16L1-RGD. The pseudovirus can escape from organic cleaning mechanism at the maximum, be exclusively absorbed by new vascular endothelial cells of a tumor and tumor cells, play the roles of inhibiting tumor neovascularization and inducing the apoptosis of the tumor cells, and can be used for preparing antineoplastic.

The invention discloses a nano bionic enhanced gram-positive bacterium capturing-separating agent as well as a preparation method and application thereof. The capture-separation agent comprises a Fe3O4 magnetic core, and the exterior of the Fe3O4 magnetic core is coated with tannic acid; the tannic acid is granular, so that the whole separating agent is a separating agent with a structure similar to a virus structure, wherein the particles can capture and separate bacteria in fruit juice. According to the invention, the nano material can specifically recognize gram-positive bacteria and can capture and separate gram-positive bacteria in fruit juice, the capture efficiency of the nano capture separating agent is remarkably improved through a viral structure and tannic acid modification, and high specificity is achieved in gram-positive bacterium adsorption and separation.

The present invention provides recombinant T4 bacteriophage expressing infectious chicken bursal disease virus structure protein VP2. The recombinant T4 bacteriophage has the nucleotide sequence of infectious bursal disease virus VP2 gene and the amino acid residue sequence of VP2 protein. The present invention also relates to the application of the recombinant T4 bacteriophage in the immunity with infectious bursal disease vaccine and the detection of infectious bursal disease virus.

The invention provides recombinant hansenula polymorpha capable of expressing Zika virus E protein under the assistance of a molecular chaperone and a construction method of the recombinant hansenulapolymorpha. The construction method comprises the following steps: firstly, constructing a coexpression vector of recombinant Zika virus structural protein E and molecular chaperone hansenula polymorpha calcium connexin, wherein the sequence of the recombinant Zika virus structural protein E is shown as SEQ.ID.NO.1 and the gene fragment of the hansenula polymorpha calcium connexin is shown as SEQ.ID.NO.2; and secondly, transforming the linearized coexpression vector into a hansenula polymorpha cell through electroporation transformation, performing induction culture, purifying a recombinant hansenula polymorpha fermentation product, and then detecting that the recombinant protein content can reach 12.6 mg / L.

The invention relates to the technical field of graphic processing, in particular to a web-based chilled electron microscope data analysis graphic system and method. The system comprises a visualization module, a segmentation module and a matching module. The visualization module comprises volume rendering, iso-surface and crystal model visualization, and chilled electron microscope data comprisesdensity data and crystal data. According to the invention, the visualization module can display data content; data content can be visually seen; the whole three-dimensional data volume is segmented according to the characteristics of a polyhedron structure, global segmentation of molecules is achieved through a multi-target fast marching method, the segmentation speed is increased, the situationthat virus structure characteristics are rapidly obtained for biologists through manpower is reduced, and memory consumption in an automatic segmentation algorithm is effectively reduced through a newdata structure; the multi-target fast marching method supports simultaneous segmentation of a multi-target region; the algorithm directly provides automatic selection of initial seed points; and a second step is added in the loop to ensure that each point is not segmented by a plurality of regions.

The invention discloses a lentivirus dissolution buffer solution and application thereof. The lentivirus dissolution buffer solution comprises 3-10 mM of Tris, 15-35 mM of proline, 1-5 mM of MgCl2, 5-20% of lactose, 0.005-0.02% of Tween 80 and the balance of water, the percentage is mass percent, and the pH value of the lentivirus dissolution buffer solution is 7-9. The components of the lentivirus dissolution buffer solution are all pharmaceutic adjuvants, the influence on the lentivirus activity can be avoided, and the virus structure can be stabilized and the virus activity can be enhanced by adding proline into the lentivirus dissolution buffer solution; the Tween80 can be used for preventing protein or virus aggregation; and MgCl2 has the effect of maintaining protein or virus activity, through the combined effect of several components in the formula, the activity and recovery rate of lentivirus during purification can be comprehensively improved, and the transduction efficiency of lentivirus and the positive rate of transduction CAR can be improved.