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Nano bionic enhanced gram-positive bacterium capturing-separating agent as well as preparation method and application thereof

A Gram-positive bacteria and nano-bionic technology, applied in the field of nano-magnetic separation, can solve the problems of bacterial lysis residue, complex equipment, and quality change of sterilization technology, and achieve the effect of rapid selectivity, simple operation, and broad application

Active Publication Date: 2021-07-20
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the above-mentioned shortcoming of the prior art, provide a kind of nano-bionic enhanced Gram-positive bacteria capture-separation agent and its preparation method and application, to solve the quality change of the sterilization technology in the prior art, equipment complex and The problem of bacterial lysis residue

Method used

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  • Nano bionic enhanced gram-positive bacterium capturing-separating agent as well as preparation method and application thereof
  • Nano bionic enhanced gram-positive bacterium capturing-separating agent as well as preparation method and application thereof
  • Nano bionic enhanced gram-positive bacterium capturing-separating agent as well as preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0053] Step 1, synthesize ferroferromagnetic nanomaterials (Fe 3 o 4 NPs). Fully and uniformly dissolve 2.72g of ferric chloride hexahydrate and 7.20g of sodium acetate in 80mL of ethylene glycol. Add 0.25 g of polyacrylic acid, and fill with nitrogen for 2 hours under magnetic stirring. The mixture was transferred to a polytetrafluoroethylene-lined stainless steel autoclave, and the autoclave was heated to 120 °C for 2 hours, then heated to 200 °C for 10 hours. After the reaction kettle is naturally cooled to room temperature, the obtained black product is washed with absolute ethanol until the supernatant is clear and transparent, and vacuum-dried at 60°C to obtain Fe 3 o 4 Magnetic Nanomaterials.

[0054] Step 2, 200mg Fe 3 o 4 Sonicate in 200mL deionized water for 15min to disperse evenly, add 252mg oxalic acid to etch for 23min under mechanical stirring, wash the obtained material with deionized water, and vacuum dry to obtain viral vFe 3 o 4 Magnetic Nanomateri...

Embodiment 2

[0077] 1. Synthesis of Fe3O4 magnetic nanomaterials by solvothermal method 3 o 4 NPs). Fully and uniformly dissolve 2.72g of ferric chloride hexahydrate and 7.20g of sodium acetate in 80mL of ethylene glycol. Add 0.25 g of polyacrylic acid, and fill with nitrogen for 2 hours under magnetic stirring. The mixture was transferred to a polytetrafluoroethylene-lined stainless steel autoclave, and the autoclave was heated to 120 °C for 2 hours, then heated to 200 °C for 10 hours. After the reaction kettle is naturally cooled to room temperature, the obtained black product is washed with absolute ethanol until the supernatant is clear and transparent, and vacuum-dried at 60°C to obtain Fe 3 o 4 Magnetic Nanomaterials.

[0078] 2. Sonicate 200mg in 200mL deionized water for 15min to disperse evenly, Fe 3 o 4 The mass ratio to deionized water is 1:1; add 252 mg oxalic acid to the dispersion under mechanical stirring for 25 min, Fe 3 o 4 The mass ratio of oxalic acid to oxalic...

Embodiment 3

[0080] 1. Synthesis of Fe3O4 magnetic nanomaterials by solvothermal method 3 o 4 NPs). Fully and uniformly dissolve 2.72g of ferric chloride hexahydrate and 7.20g of sodium acetate in 80mL of ethylene glycol. Add 0.25 g of polyacrylic acid, and fill with nitrogen for 2 hours under magnetic stirring. The mixture was transferred to a polytetrafluoroethylene-lined stainless steel autoclave, and the autoclave was heated to 110°C for 2 hours, then heated to 190°C for 10 hours. After the reaction kettle is naturally cooled to room temperature, the obtained black product is washed with absolute ethanol until the supernatant is clear and transparent, and vacuum-dried at 60°C to obtain Fe 3 o 4 magnetic nanomaterials.

[0081] 2. Sonicate 200mg in 200mL deionized water for 15min to disperse evenly, Fe 3 o 4 The mass ratio to deionized water is 1:1; add 252 mg oxalic acid to the dispersion under mechanical stirring for 22 min, Fe 3 o 4 The mass ratio of oxalic acid to oxalic a...

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Abstract

The invention discloses a nano bionic enhanced gram-positive bacterium capturing-separating agent as well as a preparation method and application thereof. The capture-separation agent comprises a Fe3O4 magnetic core, and the exterior of the Fe3O4 magnetic core is coated with tannic acid; the tannic acid is granular, so that the whole separating agent is a separating agent with a structure similar to a virus structure, wherein the particles can capture and separate bacteria in fruit juice. According to the invention, the nano material can specifically recognize gram-positive bacteria and can capture and separate gram-positive bacteria in fruit juice, the capture efficiency of the nano capture separating agent is remarkably improved through a viral structure and tannic acid modification, and high specificity is achieved in gram-positive bacterium adsorption and separation.

Description

technical field [0001] The invention belongs to the technical field of nano-magnetic separation, and in particular relates to a nano-bionic enhanced Gram-positive bacteria capture-separation agent and its preparation method and application. Background technique [0002] Food contamination by pathogenic microorganisms is an important problem worldwide and poses a serious threat to human health. Globally, foodborne diseases mediated by food spoilage microorganisms have received great attention. Foodborne bacterial outbreaks are one of the leading causes of illness and death worldwide, posing an extremely serious threat to health and safety, and imposing a heavy economic burden on healthcare and socioeconomics. Foodborne pathogens are an important source of food safety problems, and as a major contributor to foodborne illness, despite quality control, pasteurization, and ultra-high temperature (UHT) Many food safety incidents caused by contaminated and spoiled products. [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L2/80A23L2/42
CPCA23L2/80A23L2/42A23V2002/00A23V2250/1592Y02A50/30
Inventor 张文涛康怡王建龙石硕苏泽辉
Owner NORTHWEST A & F UNIV
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