Reagent composition used for preserving virus sample, and preservation method

Abstract

The invention provides a reagent composition used for preserving the nucleic acid integrity of viruses in body fluid, and application and a method of the reagent composition. The reagent composition comprises urea, lauryl sodium sulfate, trans-1.2-cyclohexanediamine tetraacetic acid, Tris-HCl, propyl p-hydroxybenzoate, diazolidinyl urea, sodium acetate, ethyl alcohol, saccharose, protease K and phenol red. After the above reagent composition and a liquid virus sample are evenly mixed, long-term preservation can be realized under a constant-temperature condition. The reagent composition and the preservation method provided by the invention can damage a virus structure to release the nucleic acid, the reagent composition contains ingredients capable of inhibiting the enzyme activity of the nucleic acid to avoid nucleic acid degradation, meanwhile, a buffering system capable of stably preserving the nucleic acid for a long term is provided, preservation time is long, and an applicable temperature range is wide.

Description

technical field [0001] The invention relates to the field of biotechnology, more specifically to a reagent composition for preserving the integrity of viral nucleic acid in body fluids. Background technique [0002] Virus detection technology can reflect the nucleic acid level of the virus, infection status, infectivity and evaluate the effect of treatment, which plays a key role in the diagnosis, treatment and administration of diseases. Judging and selecting the appropriate type of antiviral drug for the patient based on the test results, and formulating an appropriate treatment plan; according to the dynamic changes in the amount of viral nucleic acid, it provides important references for the dose, time, and whether combined medication is required for the patient. Virus detection technology has very important applications in the evaluation and observation of the efficacy of antiviral drug treatment, the efficacy of immune-enhancing drugs, and the judgment of prognosis. ...

AI Technical Summary

Problems solved by technology

Among them, simple body fluid samples have complex components, which require nucleic acid extraction in a short period of time, and long-term storage may easily lead to degradation of viral nucleic acids; and sample preservation solutions are generally isotonic saline solution or phosphate buffer saline, which has simple components and is mixed with virus samples. It requires low-temperature transportation and cannot be stored for a long time, and may even cause degradation of viral nucleic acid in a short period of time, which will affect the subsequent nucleic acid extraction efficiency and even PCR results

[0005] Therefore, research and development of a preservation solution that preserves the integrity of viral nucleic acids in body fluids can specifically solve the problem of nucleic acid degradation that may be caused by sampling and transportation of virus samples in body fluids, and improve the sensitivity and accuracy of virus detection using fluorescent quantitative PCR.

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