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Indirect enzyme-linked immunosorbent assay (ELISA) kit for detecting human West Nile virus and detection method thereof

A West Nile virus and kit technology, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of difficulty in popularization and promotion, time-consuming, complicated experimental operation, etc. good effect

Inactive Publication Date: 2011-09-14
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the above-mentioned techniques and methods can detect WNV and its antibody levels, and have achieved certain results in practice, they all have complex test operations, time-consuming, specific professional skills and equipment, etc., and are often limited to laboratories. Difficult to popularize and promote

Method used

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  • Indirect enzyme-linked immunosorbent assay (ELISA) kit for detecting human West Nile virus and detection method thereof
  • Indirect enzyme-linked immunosorbent assay (ELISA) kit for detecting human West Nile virus and detection method thereof
  • Indirect enzyme-linked immunosorbent assay (ELISA) kit for detecting human West Nile virus and detection method thereof

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Embodiment Construction

[0032] Such as Figure 1 to Figure 6 as shown, Figure 4A SDS-PAGE results, lane 1 is normal 293T cells sonicated, centrifuged supernatant, filtered and concentrated by ultrafiltration tube; lane 2 is protein marker; lane 3 is 293T cell culture co-expressing West Nile virus envelope protein The supernatant was filtered and concentrated by an ultrafiltration tube; lane 4 was the sample after sonication of 293T cells co-expressing the envelope protein of West Nile virus, centrifugation of the supernatant, filtration and concentration by an ultrafiltration tube. Figure 4B Western blot hybridization was performed with West Nile virus E monoclonal antibody (Millipore). Swimming lane 1 is the 293T cell culture supernatant co-expressed West Nile virus envelope protein filtered, ultrafiltration tube concentrated sample; Swimming lane 2 is the 293T cell co-expressed West Nile virus envelope protein sonicated, centrifuged supernatant, Samples after filtration and concentration by ult...

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Abstract

The invention discloses an indirect enzyme-linked immunosorbent assay (ELISA) kit for detecting a human West Nile virus and a detection method thereof. West Nile viruslike particles expressed by a mammal cell expression system provide an ELISA method for coating an antigen; and the viruslike particles (VLPs) are hollow particles comprising one or more virus structure protein, are nucleic acid without viruses, and has conformation and antigenic epitope as same as those of a natural virus, so the West Nile viruslike particles have no infectivity to a human body, high stability, high safety and immunogenicity closer to that of the natural virus, and is difficult to inactivate. A kit with safety, specificity, sensitivity, quickness, simpleness in operation and economy is provided for detecting a human West Nile virus IgG antibody; and a technical method is provided for preventing and controlling importation of the West Nile virus from foreign countries to China.

Description

technical field [0001] The invention relates to the fields of virology, molecular biology and biological products, in particular to an indirect ELISA kit for detecting human West Nile virus IgG antibody and a detection method thereof. Background technique [0002] West Nile Virus (WNV) is a Flavivirus belonging to the family Flaviviridae. It is a single-stranded positive-strand RNA virus. The expressed protein can be divided into 10 separate protein structures: 3 structural proteins, envelope Protein (E), membrane protein (M) and nucleocapsid protein (C), as well as seven non-structural proteins NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5 were similar in structure to flaviviruses such as dengue virus. After West Nile virus infects the human body, it can cause an acute infectious disease called "West Nile fever". This virus can cause symptoms such as high fever, headache, muscle pain, rash, and swollen lymph nodes. It can invade the central nervous system and produce symptoms o...

Claims

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Application Information

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IPC IPC(8): G01N33/543
Inventor 胡孔新咸庆杰郭雪杨鹏飞平芮巾张丽萍马雪征
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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