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Structure of recombined T4 bacteriophage of expression infectious cysticercosis virus VP2 protein and its use

A phage, infectious technology, applied in the field of recombinant T4 phage, to achieve the effect of strong immunogenicity, high copy number and easy purification

Inactive Publication Date: 2004-12-15
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the expression of IBDV structural proteins by phage

Method used

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  • Structure of recombined T4 bacteriophage of expression infectious cysticercosis virus VP2 protein and its use
  • Structure of recombined T4 bacteriophage of expression infectious cysticercosis virus VP2 protein and its use
  • Structure of recombined T4 bacteriophage of expression infectious cysticercosis virus VP2 protein and its use

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Experimental program
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Embodiment Construction

[0031] 1. Construction of integrated plasmid pR-VP2

[0032] The pR plasmid (Ren et al, 1996) was used to construct the recombinant integration plasmid pR-VP2. The PCR product of the vp2 gene and the plasmid pR were respectively digested with EcoRI at 37° C., and then the digested PCR product of vp2 and the plasmid pR were ligated under the action of T4 ligase. Escherichia coli DH5α was transformed with the ligation product. Conversion method to CaCl 2 Law. via Amp r Resistance screening was performed to obtain recombinants inserted with the vp2 gene. Then use a pair of specific primers SOC-S / VP2-A for PCR screening. For the positive bacteria screened by PCR, pick a single colony and inoculate it in 3mL LA medium, and culture it at 37°C with shaking at 200r / m for 16-20 hours. The plasmid was extracted with E.Z.N.A.®Plasmid Minipreps Kit, and then digested with restriction endonucleases and sequenced to obtain the recombinant integrated plasmid pR-VP2.

[0033] 2. Acquis...

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Abstract

The present invention provides recombinant T4 bacteriophage expressing infectious chicken bursal disease virus structure protein VP2. The recombinant T4 bacteriophage has the nucleotide sequence of infectious bursal disease virus VP2 gene and the amino acid residue sequence of VP2 protein. The present invention also relates to the application of the recombinant T4 bacteriophage in the immunity with infectious bursal disease vaccine and the detection of infectious bursal disease virus.

Description

technical field [0001] The invention relates to the construction and application of a recombinant T4 phage expressing chicken infectious bursal disease virus structural protein. The present invention further relates to the application of the recombinant T4 phage in the detection of infectious bursal disease vaccine and infectious bursal disease virus antibody. Background technique [0002] Infectious bursal disease virus (IBDV) belongs to the family Birnaviridae (Birnaviridae) of the genus Avibirnavirus, and it is the pathogen of chicken infectious bursal disease. It is generally believed that IBDV has four mature proteins: VP1, VP2, VP3 and VP4, with molecular weights of 90kDa, 37-40kDa, 32-35kDa and 24-30kDa, respectively. Among them, VP2 and VP3 are the main structural proteins, accounting for 51% and 40% of the total viral proteins, respectively. The molecular weight of VP2 is about 40kDa. It contains serotype-specific antigenic determinants tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/12C12N7/01C12N15/40C12N15/73
Inventor 曹永长毕英佐史泉城马静云
Owner SOUTH CHINA AGRI UNIV
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