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Application of ADDA and/or DMTA in preparing medicine for improving expression level of gamma-globin

A technology of globin and drugs, applied in the direction of drug combinations, pharmaceutical formulas, medical preparations containing active ingredients, etc.

Inactive Publication Date: 2012-05-23
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] There is no report on the use of ADDA or DMTA for the preparation of gamma-globin inducers

Method used

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  • Application of ADDA and/or DMTA in preparing medicine for improving expression level of gamma-globin
  • Application of ADDA and/or DMTA in preparing medicine for improving expression level of gamma-globin
  • Application of ADDA and/or DMTA in preparing medicine for improving expression level of gamma-globin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Materials and methods

[0022] 1) Compound

[0023] ADDA (adenosine-2', 3'-dialdehyde) and DMTA (5'-deoxy-5'(methyl-thio)-adenosine) were purchased from Sigma Company in the United States. ADDA was dissolved in deionized water to make a 10mM stock solution; DMTA was dissolved in DMSO (dimethyl sulfoxide) to make a 0.5M stock solution.

[0024] 2) Cell culture

[0025] Human erythroid K562 cells were cultured in RPMI1640 medium at 37°C, 10% calf serum, 10 5 U / L penicillin, 100mg / L streptomycin, 5% CO 2 . K562 cells were treated with a final concentration of 50μM ADDA or 0.1mM DMTA for 72 hours, and the cells were collected.

[0026] Human bone marrow CD34 + Cells were separated with CD34 immunomagnetic beads (Miltenyi Biotec, USA), 50μM ADDA or 100μM DMTA was added, normal saline or DMSO (volume ratio 2 . Specifically, the cytokine SCF (100ng / ml), IL-3 (10ng / ml) and Flt-3 (10ng / ml) were added to the culture medium for 1 week, and then replaced with only the cytokine EPO (5U / ...

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Abstract

The invention belongs to the technical field of biological medicine, discloses an application of ADDA and / or DMTA in preparing medicine for improving expression level of gamma-globin, and provides a candidate drug for curing anemia caused by beta-thalassemia, sickle-cell anemia, hematopoietic system tumor and hematopoietic dysfunction.

Description

Technical field [0001] The present invention belongs to the technical field of biomedicine, and relates to ADDA (adenosine-2', 3'-dialdehyde, 2', 3'-dialdehyde adenosine) and / or DMTA (5'-deoxy-5'(methyl-thio)adenosine) , 5'-deoxy-5'-methylthioadenosine), especially related to the application of ADDA and / or DMTA in the preparation of drugs that increase the expression of γ-globin, and in the preparation and treatment of β-thalassemia And sickle cell anemia, hematopoietic system disorders or tumor-caused anemia drugs. Background technique [0002] Hemoglobin in adults consists of two α-globin subunits and two β-globin subunits (α2β2). The human β-like globin gene cluster is located on the short arm of chromosome 11 and contains five globin genes (ε, G γ, A γ, δ, β), encoding β-like globin to synthesize hemoglobin. In the process of human ontogeny, each globin gene is turned on and expressed strictly according to the specific development period and tissue specificity. From concep...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7076A61P7/06
Inventor 赵权马驰何颖红
Owner NANJING UNIV
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