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Method for breeding transgenic paddy rice with changeable leaf angle and special recombinant carrier thereof

A technology of recombining vectors and genes, applied in the biological field, can solve the problems of increasing yield and achieve the effect of increasing rice yield

Inactive Publication Date: 2011-11-16
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After screening more than 100 mutants, people found the weak mutant d61-7 of OsBRI1. Although the mutant can increase the biomass of rice by dense planting, it does not increase the yield because d61-7 produces small seeds.

Method used

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  • Method for breeding transgenic paddy rice with changeable leaf angle and special recombinant carrier thereof
  • Method for breeding transgenic paddy rice with changeable leaf angle and special recombinant carrier thereof
  • Method for breeding transgenic paddy rice with changeable leaf angle and special recombinant carrier thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Example 1. Obtaining and Detection of Transgenic Rice with Smaller Leaf Angle

[0030] 1. Obtaining transgenic rice with smaller leaf angle

[0031] 1. Obtaining the cDNA of OsBAK1

[0032] 1) Extraction of rice total RNA

[0033] Since RNA is easily degraded, the organic solvent must be newly purchased, and ddH treated with DEPC 2 O Prepare the required reagents. All glassware used were baked in an oven at 180°C for more than 8 hours, and disposable gloves were frequently changed during operation.

[0034] Grind 100mg of material in liquid nitrogen, transfer to a 1.5ml centrifuge tube containing 1ml Trizol reagent after grinding, mix well, and place at room temperature for 5min; add 0.2ml of fresh chloroform to each tube, shake vigorously for 15sec, and let stand at room temperature 2-3min, ≤12,000rpm (not less than 10,000rpm), 4°C, centrifuge for 15min; transfer the upper colorless aqueous phase to a new 1.5ml centrifuge tube, use 0.6ml isopropanol to precipitate ...

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Abstract

The invention discloses a method for breeding transgenic paddy rice with changeable leaf angle and a special recombinant carrier thereof. The recombinant carrier contains Os BAK1-1 gene segment, wherein the Os BAK1-1 gene segment is obtained by the KpnI and SacI double enzyme digestion of the gene expressed by sequence 1 in a sequence table. The recombinant carrier is induced into the paddy rice to obtain the transgenic paddy rice, wherein the leaf of the transgenic paddy rice is erect, thereby being capable of improving yield per unit.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for cultivating transgenic rice with changed leaf angle and a special recombinant vector thereof. Background technique [0002] Ideal plant architecture is the decisive factor for increasing rice yield. In the ideal plant type of rice, semi-dwarf and erect leaves are two very favorable traits for increasing yield (Wang Y and Li J,. 2008, Annu. Rev. Plant Biol., 59: 253-279). The Green Revolution is a successful example of improving rice yield by improving rice plant type. Some recent studies have shown that leaf erection is also one of the goals of achieving high-yielding rice plant architecture. Upright leaves can increase the photosynthetic rate of the canopy, improve the illumination of the lower part of the group, and increase the material production; at the same time, it can increase the amount of light at the base of the canopy, enhance the vitality of the root syste...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/84C12N1/21A01H5/00C12R1/01C12R1/19
Inventor 种康李丹黎家许智宏
Owner INST OF BOTANY CHINESE ACAD OF SCI