Plant cultivating method for dynamically observing and measuring roots in situ
A technology of dynamic observation and cultivation method, applied in the fields of root biology, plant nutrition, crop cultivation and plant physiology, which can solve the problems of difficult in situ observation and measurement, opaque root structure of soil medium, and no technical reports.
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Embodiment 1
[0037] The in-situ observation and determination of the morphological configuration of the soybean root system of embodiment 1
[0038] (1) Design transparent cultivation containers and growth media;
[0039] as attached figure 1 As shown, a transparent cylindrical plexiglass container 1 is adopted, with a height of 20 cm and a diameter of 12 cm. An openable cover 2 is provided, and a through hole 3 with a diameter of 20 mm is arranged in the middle of the cover;
[0040] The transparent medium is modified 1 / 2 Hoagland nutrient solution + 0.15% Phytagel TM (W / V) + different phosphorus treatments; the different phosphorus treatments include no phosphorus treatment or 1.0mM KH 2 PO 4 For processing, refer to the conventional techniques of those skilled in the art, adjust the pH value to 5.8, and use hydrochloric acid or sodium hydroxide and other reagents to adjust according to conventional techniques.
[0041] The composition of 1 / 2 Hoagland nutrient solution is (μM): KNO ...
Embodiment 2
[0060] The in-situ observation and determination of the morphological configuration of the rice root system of embodiment 2
[0061] (1) Design transparent cultivation containers and growth media;
[0062] Adopt a transparent cylindrical plexiglass container with a height of 20cm and a diameter of 12cm, with an openable cover, and a through hole with a diameter of 20mm in the middle of the cover;
[0063] The rice medium is: the rice nutrient solution improved by Yoshida et al. in 1976 + 0.15% Phytagel TM (W / V) + different phosphorus treatments including no phosphorus treatment or 1.0mM KH 2 PO 4 For processing, refer to the conventional techniques of those skilled in the art, adjust the pH value to 5.7, and use hydrochloric acid or sodium hydroxide and other reagents to adjust according to conventional techniques.
[0064] The composition of rice nutrient solution improved by Yoshida 1976 is (μM): H 3 BO 3 19, Ca(NO 3 ) 2 4H 2 O 1000, NH 4 NO 3 429, (NH 4 ) 6 Mo...
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