Plant cultivating method for dynamically observing and measuring roots in situ

A technology of dynamic observation and cultivation method, applied in the fields of root biology, plant nutrition, crop cultivation and plant physiology, which can solve the problems of difficult in situ observation and measurement, opaque root structure of soil medium, and no technical reports.

Inactive Publication Date: 2011-07-27
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, some progress has been made in the research on the root system, but under natural growth conditions, due to the opacity of the soil medium and the complexity of the root system structure, it is difficult to observe and measure in situ (Ma Xinming et al., 2003; Zhu et al., 2006) )
[0004] Most of the root morphology and configuration data reported so far are data obtained by destructive sampling, not in situ data, and most of the studies on root architecture and nutrient efficiency are qualitative studies, and there are few quantitative studies on root architecture and nutrient efficiency. See the report
In addition, most of the existing research is the study of root architecture data and nutrient efficiency at a certain moment, and there are no technical reports on the dynamic in-situ root architecture data and nutrient efficiency.

Method used

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  • Plant cultivating method for dynamically observing and measuring roots in situ
  • Plant cultivating method for dynamically observing and measuring roots in situ
  • Plant cultivating method for dynamically observing and measuring roots in situ

Examples

Experimental program
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Effect test

Embodiment 1

[0037] The in-situ observation and determination of the morphological configuration of the soybean root system of embodiment 1

[0038] (1) Design transparent cultivation containers and growth media;

[0039] as attached figure 1 As shown, a transparent cylindrical plexiglass container 1 is adopted, with a height of 20 cm and a diameter of 12 cm. An openable cover 2 is provided, and a through hole 3 with a diameter of 20 mm is arranged in the middle of the cover;

[0040] The transparent medium is modified 1 / 2 Hoagland nutrient solution + 0.15% Phytagel TM (W / V) + different phosphorus treatments; the different phosphorus treatments include no phosphorus treatment or 1.0mM KH 2 PO 4 For processing, refer to the conventional techniques of those skilled in the art, adjust the pH value to 5.8, and use hydrochloric acid or sodium hydroxide and other reagents to adjust according to conventional techniques.

[0041] The composition of 1 / 2 Hoagland nutrient solution is (μM): KNO ...

Embodiment 2

[0060] The in-situ observation and determination of the morphological configuration of the rice root system of embodiment 2

[0061] (1) Design transparent cultivation containers and growth media;

[0062] Adopt a transparent cylindrical plexiglass container with a height of 20cm and a diameter of 12cm, with an openable cover, and a through hole with a diameter of 20mm in the middle of the cover;

[0063] The rice medium is: the rice nutrient solution improved by Yoshida et al. in 1976 + 0.15% Phytagel TM (W / V) + different phosphorus treatments including no phosphorus treatment or 1.0mM KH 2 PO 4 For processing, refer to the conventional techniques of those skilled in the art, adjust the pH value to 5.7, and use hydrochloric acid or sodium hydroxide and other reagents to adjust according to conventional techniques.

[0064] The composition of rice nutrient solution improved by Yoshida 1976 is (μM): H 3 BO 3 19, Ca(NO 3 ) 2 4H 2 O 1000, NH 4 NO 3 429, (NH 4 ) 6 Mo...

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Abstract

The invention discloses a plant cultivating method for dynamically observing and measuring roots in situ, which comprises the following steps: designing a transparent cultivation container and growth media, seed sterilization and pregermination, transferring seedlings to the transparent cultivation container after budding, keeping plant roots naturally growing in the transparent growth media and the like, thus facilitating the adoption of a three-dimensional laser scanner to scan and in-situ observation and image capture of the three-dimensional configuration of the roots. In the invention, the design of suitable transparent growth environment ensures the plant roots to naturally grow in the transparent growth environment, thus facilitating subsequent three-dimensional visual analysis of the in-situ growth state of the plant roots and directly reflecting the three-dimensional growth state of the roots.

Description

technical field [0001] The invention belongs to the fields of root biology, plant nutrition, crop cultivation and plant physiology, and in particular relates to a plant cultivation method for in-situ dynamic observation and measurement of roots. Background technique [0002] The root system is the main organ for plants to absorb nutrients and water, and only well-grown roots can meet the needs of plant growth (Barber, 1995; Bailey et al, 2002; Lynch, 2007). Therefore, the study of plant root growth is of great significance to understand the nutrient requirements of plants and their relationship with the environment. [0003] At present, some progress has been made in the research on the root system, but under natural growth conditions, due to the opacity of the soil medium and the complexity of the root system structure, it is difficult to observe and measure in situ (Ma Xinming et al., 2003; Zhu et al., 2006) ). [0004] Most of the root morphology and configuration data ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G31/00C05G1/00A01G7/00A01G31/02
CPCY02P60/21
Inventor 方素琴廖红严小龙
Owner SOUTH CHINA AGRI UNIV
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